Laboratory of Cell Biology

Michael M. Gottesman, M.D.
Deputy Chief
Suresh V. Ambudkar, Ph.D.

The Laboratory of Cell Biology (LCB) studies the processing, transport, and metabolism of proteins and small molecules related to malignant transformation, metastasis, and multidrug resistance in cancer. The principal investigators of the laboratory, who are experts in molecular biology, genetics, biochemistry, structural biology, cellular regulation of cell growth and metabolism, resistance to anticancer drugs, and the physics of cell-matrix interactions, work on research projects related to those topics. The Multidrug Resistance Section studies the molecular basis of anticancer drug resistance, while the Transport Biochemistry Section investigates the biochemistry of energy-dependent transporters. Post-translational regulation of the tumor suppressor p53 and the roles of Wip1 in promoting cellular proliferation are the focus of the Chemical Immunology Section, while the DNA and Nucleoproteins Section is involved in computational and experimental studies of nucleic acid-protein interactions. The Biophysics Section develops novel EM-based approaches to determine single molecule and multisubunit structures and the Crystallography Section works on X-ray crystallography of membrane proteins and protein complexes. The Tissue Morphodynamics Unit studies how normal and cancer cells modify their environments to promote normal differentiation and cancer cell metastasis. Finally, our newest group, the RNA Metabolism and Epitranscriptomics Unit, investigates how the RNA epigenome modulates gene expression during development and when confronted by disease. (Please see webpages for LCB investigators for further details.) The LCB also includes three Cores, one involved in 3D electron microscopy, one in molecular modeling, and one in characterization of post-transcriptional protein processing. Joint journal clubs and data presentations among some sections, and laboratory-wide research seminars facilitate the sharing of expertise and help to foster collaborations among LCB staff.

Laboratory of Cell Biology Staff - 2017

Laboratory of Cell Biology Staff - 2017






There are no Open Positions at this time, check back again later.

Symposium held in September:

Celebrating 30 Years of Research on Multidrug Resistance and ABC Transporters 

September 22-23, 2016, NIH Campus, Building 35, Room 620-630

The discovery and characterization of P-glycoprotein as a mechanism of multidrug resistance is widely accepted as a seminal contribution to the ongoing effort to end death and alleviate suffering caused by cancer. In honor of the 30th anniversary of the cloning of the human MDR1 (ABCB1) gene, we held a day-and-a-half scientific symposium on the NIH campus in Bethesda, Maryland, on September 22 and 23, 2016.  This event included current and former members of the MDR group in the Laboratory of Cell Biology, as well as collaborators and colleagues in the field.  The first day of scientific talks and discussions was followed by a dinner in celebration of this anniversary.