Regulation of gene expression is the cardinal control mechanism that ensures organismal development. Once thought to be a passive player in the flow of genetic information, RNA has emerged as an essential core component in numerous gene regulatory pathways. We want to understand the mechanisms by which the RNA epigenome modulates gene expression during development and in disease. Major interests of the lab include:

Determine the effect of RNA modifications on RNA biogenesis and function. More than 100 RNA modifications have been described so far (Li and Mason, 2014). While many of them have been linked to human disease, the underlying mechanisms regulating why RNA molecules are modified, or how these modifications affect RNA maturation and function remain largely unknown. We want to understand how RNAs are targeted for modification and how modified RNA transcripts are ‘identified’ by the cellular machinery.

Delineate m6A-dependent cellular pathways that control cell fate decisions. The RNA modification m6A is required for differentiation of embryonic stem cells into distinct cell fates. We want to understand, at the molecular level, how loss of m6A disrupts gene regulatory networks and abrogates the cells’ capacity to differentiate.

Elucidate the molecular interactions between metabolism, RNA modifications and gene expression. Regulation of RNA modifications requires energy and factors shared with metabolic pathways, enabling the exchange of information between gene expression programs and metabolic status. We will explore how changes in the cellular environment and metabolism impact gene expression through RNA post-transcriptional modifications.