Mass Spectrometry Resource

The Mass Spectrometry Resource aims to advance research by bringing cutting-edge protein-based technologies to the NCI CCR community to facilitate basic and translational research. Although mass spectrometry is a powerful technique, experimental design and sample preparation are critical for a successful outcome. By making available our expertise in mass spectrometry, we seek to help researchers with their proteomics experiments, beginning with initial experimental design and sample preparation and continuing through data interpretation and design of follow-up experiments. We collaborate on projects that explore protein-protein and protein-nucleic acid complexes, identify sites of protein post-translational modification or reaction with a small molecule, and quantify global changes in protein level upon protein knock-down/overexpression or cellular treatment. We also collaborate on projects using structural mass spectrometry approaches to study changes in protein conformation.

CCR Molecular Modeling Core

The CCR Molecular Modeling Core, under the direction of Dr. Stewart Durell, was established to provide a convenient way for experimentalists to take advantage of molecular modeling and computational biology. In the framework of consultations, scientists can explore how such tools could benefit their projects. The Core then acts as a clearinghouse to help set up and use appropriate software, and/or form collaborations with other scientists. Upon mutual agreement, Core personnel will enter into a collaboration to do the modeling work themselves. All services of the Core are free of charge.

NIH Intramural Cryo-EM (NICE) Consortium

The NIH Intramural Cryo-EM (NICE) Consortium, under the direction of Dr. Rick Huang (huangrk@nih.gov), currently serves intramural investigators in the NCI, NIAID, NIEHS, NICHD, NIDCR, NEI, and NIA.  This facility provides access to a state-of-the-art Titan Krios cryo electron microscope for atomic-resolution structure determination of protein, macromolecular complexes and receptors, cellular organelles, and infectious pathogens using a single particle or cryo- tomography approach.  Detailed 3D maps computed by electron micrographs often spark biological insights in cancer research, vaccine design, cell development, and human physiological and functional studies.  In addition to high-resolution data collection service, our microscopists offer technical consultation and guidance to Intramural trainees on cryo-specimen preparation and image processing . We are proud to be a member of a global collaboration to provide cutting-edge microscopy access and to share our expertise with the NIH structural biology community to advance our knowledge in the life sciences.