Laboratory of Cell Biology
Laboratory of Cell Biology
About
The Laboratory of Cell Biology (LCB) studies the processing, transport, and metabolism of proteins and small molecules related to malignant transformation, metastasis, and multidrug resistance in cancer. The principal investigators of the laboratory, who are experts in molecular biology, genetics, biochemistry, structural biology, cellular regulation of cell growth and metabolism, resistance to anticancer drugs, and the physics of cell-matrix interactions, work on research projects related to those topics. The Multidrug Resistance Section studies the molecular basis of anticancer drug resistance, while the Transport Biochemistry Section investigates the biochemistry of energy-dependent transporters. Post-translational regulation of the tumor suppressor p53 and the roles of Wip1 in promoting cellular proliferation are the focus of the Chemical Immunology Section, while the DNA and Nucleoproteins Section is involved in computational and experimental studies of nucleic acid-protein interactions. The Crystallography Section works on X-ray crystallography of membrane proteins and protein complexes and the Tissue Morphodynamics Unit studies how normal and cancer cells modify their environments to promote normal differentiation and cancer cell metastasis. Finally, our newest group, the RNA Metabolism and Epitranscriptomics Unit, investigates how the RNA epigenome modulates gene expression during development and when confronted by disease. (Please see webpages for LCB investigators for further details.) The LCB also includes three Cores, one that deals with molecular modeling, one with mass spectrometry, and one that provides cryo-EM analysis. Joint journal clubs and data presentations among some sections, and laboratory-wide research seminars facilitate the sharing of expertise and help to foster collaborations among LCB staff.
Job Vacancies
We have no open positions in our group at this time, please check back later.
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News
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Cores
Mass Spectrometry Resource
The Mass Spectrometry Resource aims to advance research by bringing cutting-edge protein-based technologies to the NCI CCR community to facilitate basic and translational research. Although mass spectrometry is a powerful technique, experimental design and sample preparation are critical for a successful outcome. By making available our expertise in mass spectrometry, we seek to help researchers with their proteomics experiments, beginning with initial experimental design and sample preparation and continuing through data interpretation and design of follow-up experiments. We collaborate on projects that explore protein-protein and protein-nucleic acid complexes, identify sites of protein post-translational modification or reaction with a small molecule, and quantify global changes in protein level upon protein knock-down/overexpression or cellular treatment. We also collaborate on projects using structural mass spectrometry approaches to study changes in protein conformation.
CCR Molecular Modeling Core
The CCR Molecular Modeling Core, under the direction of Dr. Stewart Durell, was established to provide a convenient way for experimentalists to take advantage of molecular modeling and computational biology. In the framework of consultations, scientists can explore how such tools could benefit their projects. The Core then acts as a clearinghouse to help set up and use appropriate software, and/or form collaborations with other scientists. Upon mutual agreement, Core personnel will enter into a collaboration to do the modeling work themselves. All services of the Core are free of charge.
NIH Intramural Cryo-EM (NICE) Consortium
The NIH Intramural Cryo-EM (NICE) Consortium, under the direction of Dr. Rick Huang (huangrk@nih.gov), currently serves intramural investigators in the NCI, NIAID, NIEHS, NICHD, NIDCR, NEI, and NIA. This facility provides access to a state-of-the-art Titan Krios cryo electron microscope for atomic-resolution structure determination of protein, macromolecular complexes and receptors, cellular organelles, and infectious pathogens using a single particle or cryo- tomography approach. Detailed 3D maps computed by electron micrographs often spark biological insights in cancer research, vaccine design, cell development, and human physiological and functional studies. In addition to high-resolution data collection service, our microscopists offer technical consultation and guidance to Intramural trainees on cryo-specimen preparation and image processing . We are proud to be a member of a global collaboration to provide cutting-edge microscopy access and to share our expertise with the NIH structural biology community to advance our knowledge in the life sciences.
Seminars
LCB webinars/seminars are held from 2:30 to 3:30 unless otherwise noted.
January
2 No seminar
9 Jack Shern, Pediatric Oncology Branch, NCI (guest of Pedro Batista)
Genetic Tumor Progression in NF1-Based Nerve Tumors
16
23 Paula Salazar, Postdoctoral Fellow, Transport Biochemistry Section, LCB
Progress on Understanding the Inhibitor Interaction Sites of the Multidrug Transporter P-glycoprotein
30 Rick Huang, LCB, NCI Cryo-EM Core
The Secret Ingredients in CryoEM Specimen Preparation
February
6
13 Marielle Yohe, Laboratory of Cell and Developmental Signaling, NCI (guest of Pedro Batista)
RAS/MAPK Pathway Inhibitors for the Treatment of Costello Syndrome
20 Gamze Ayaz Sen, Visiting Fellow, Laboratory of Cancer Biology and Genetics, NCI (guest of Ettore Appella)
Exploring Breast Cancer Interactomes Through Proteomics: A BioID Approach
27
March
5 Natalie Porat-Shliom, Thoracic and GI Malignancies Branch, Cell Biology and Imaging Section, NCI Building 10 (guest of Pedro Batista)
How Do Hepatocytes Organize Their Metabolism?
12 LCB Site Visit practice talk
19 LCB Site Visit practice talk
26 LCB Site Visit practice talk
April
2 LCB Site Visit practice talk
9 No seminar
16 LCB Site Visit practice talk
23 LCB Site Visit practice talks
30 LCB Site Visit practice talks
May
7 Site visit IT checks
13 -15 LCB Site Visit
21
28
June
4 Yves Pommier, Developmental Therapeutics Branch, Molecular Pharmacology, Group, NCI (Guest of Michael Gottesman)
Schlafen 11 (SLFN11) at the Crossroads of DNA Damage and Innate Immune Responses
11 Paul M. Hwang, Senior Investigator, Cardiovascular and Cancer Genetics, NHLBI (guest of Di Xia)
WASF3 Disrupts Mitochondrial Respiration and may Mediate Bioenergetic Deficiency in Chronic Fatigue Syndrome
18 Postbac Presentations: Siddhardha Maligireddy and Will Frye
Trying to Fix a Broken Gene: Gene Therapy Approaches to Treating Newly Identified Splicing Disorder
&
Characterization of Zebrafish Abcc4 and the Role of CRISPR Screens in Determining Drug Mechanisms of Action
25
July
2
9 Michael Aregger, Functional Genomics Section, Molecular Targets Program, NCI Frederick (guest of Ednah Ooko)
Interrogating Genetic Interactions & Regulators of Metabolic Plasticity in Cancer Cells
16
23 Emily Arner (Guest of Pedro Batista)
NDUFA4L2 is a Metabolic Regulator of Epithelial Plasticity and Metastasis
Contact
Contact Info
Center for Cancer Research National Cancer Institute
- Building 37 Room 2108
- Bethesda, MD 20892-4256
- 240-760-6310
- 240-541-4472