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Suresh V. Ambudkar, Ph.D.

Suresh V. Ambudkar, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute

RESEARCH SUMMARY

Dr. Ambudkar and colleagues elucidate the role of ABC drug transporters in cancers that develop resistance to anti-cancer drugs. They facilitate the development of new therapeutic strategies to improve the efficiency of chemotherapy for cancer patients. Transition metal ion Forster resonance energy transfer, chemical crosslinking, directed mutagenesis, and molecular modeling techniques are used to understand the molecular mechanisms of polyspecificity, the catalytic cycle and drug transport. Monoclonal antibodies and various mutant variants are used to obtain high-resolution structures of P-glycoprotein (ABCB1). They also characterize the modulatory effect of targeted therapeutic tyrosine kinase inhibitors and non-toxic natural products to overcome ABC transporter-mediated drug resistance.

Areas of Expertise

1) ABC transporters 2) catalytic cycle 3) multidrug resistance 4) transport mechanism 5) tyrosine kinase inhibitors

Publications

Selected Key Publications

SIS3, a specific inhibitor of Smad3 reverses ABCB1- and ABCG2-mediated multidrug resistance in cancer cell lines

Wu CP, Murakami M, Hsiao SH, Liu TC, Yeh N, Li YQ, Hung TH, Wu YS, Ambudkar SV.
Cancer Lett. 433: 259-72, 2018. [ Journal Article ]

Relation between the turnover number for vinblastine transport and for vinblastine-stimulated ATP hydrolysis by human P-glycoprotein

Ambudkar SV, Cardarelli CO, Pashinsky I, Stein WD.
J. Biol. Chem. 272: 21160-6, 1997. [ Journal Article ]

Multiple transport-active binding sites are available for a single substrate on human P-glycoprotein (ABCB1)

Chufan EE, Kapoor K, Sim HM, Singh S, Talele TT, Durell SR, Ambudkar SV.
PLoS ONE. 8: e82463, 2013. [ Journal Article ]

Elucidation of the structural basis of interaction of the BCR-ABL kinase inhibitor, nilotinib (Tasigna) with the human ABC drug transporter P-glycoprotein

Shukla S, Chufan EE, Singh S, Skoumbourdis AP, Kapoor K, Boxer MB, Duveau DY, Thomas CJ, Talele TT, Ambudkar SV.
Leukemia. 28: 961-4, 2014. [ Journal Article ]

Job Vacancies

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Team

Staff Scientist
Andaleeb Sajid, Ph.D.
Postdoctoral Fellow (Visiting)
Hadiar Rahman, Ph.D.
Postdoctoral Fellow (Visiting)
Megumi Murakami, Ph.D.
Predoctoral Fellow (Graduate Student)
Barry (Jiahao) Liang
Postdoctoral Fellow (Visiting)
Paula Salazar, Ph.D.
Postdoctoral Fellow (Visiting)
Nandhini Ranganathan, Ph.D.
Biologist
Tapan Maity, Ph.D.
Post-Baccalaureate Fellow
Andrew Perciaccante