Wei-Shau Hu Appointed as a Deputy Director in HIV Dynamics and Replication Program
In October 2021, Wei-Shau Hu was appointed as HIV DRP Deputy Director of Basic Research.
Wei-Shau Hu Received 2021 Distinguished Research Career Award from The Ohio State University Center for Retrovirus Research
Wei-Shau Hu was selected by the Center for Retrovirus Research (CRR) of The Ohio State University to receive the 2021 Distinguished Research Career Award. This annual award honors the distinguished research career of a scientist working in the field of retrovirology. The retrovirologist is nominated by student and faculty members of the CRR and as part of the award recognition is invited to give a special lecture to all members of The Ohio State University biomedical research community, co-sponsored by the CRR, the Infectious Diseases Institute, the Comprehensive Cancer Center, and the campus-wide Virology Forum. Dr. Hu received the award "in recognition of her substantial body of work contributing to our understanding of retroviral recombination, RNA packaging, and virus assembly." The title of her keynote seminar was “How Does HIV-1 Transfer Genetic Information to Its Progeny?”
PNAS Video on Cozzarelli Prize Awarded to Pathak and Hu Labs
In a video produced by Proceedings of the National Academy of Sciences USA (PNAS) about the 2020 Cozzarelli Prize in Biomedical Sciences, Vinay Pathak discusses the HIV-1 uncoating discovery by his research team in collaboration with Wei-Shau Hu’s lab. The video can be viewed on the PNAS Cozzarelli Prize page and on the PNAS YouTube channel (click here).
The publication that Dr. Pathak discusses in the video (“HIV-1 uncoats in the nucleus near sites of integration,” PNAS 117:5486-5493, 2020) was also featured on the website of the Center for Cancer Research, National Cancer Institute (“New study overturns conventional understanding of how HIV infection occurs”) and highlighted on the front cover of the Cold Spring Harbor 2020 Retroviruses Meeting abstract book.
New Investigator Scholarships, Conference on Retroviruses and Opportunistic Infections
Jonathan Rawson was awarded a New Investigator Scholarship in 2021 to present his research findings in the Conference on Retroviruses and Opportunistic Infections (CROI). CROI scholarship awardees in previous years include Alice Duchon and Jonathan Rawson (2020), Chijioke (CJ) Umunnakwe (2019), Yang Liu (2017), and Luca Sardo (2015).
NIH Fellows Awards for Research Excellence
Jonathan Rawson received a 2021 NIH Fellows Award for Research Excellence (FARE) for travel to attend and present his work at a scientific meeting in the U.S. This award, which acknowledges outstanding scientific research performed by intramural postdoctoral fellows, is sponsored by the NIH Fellows Committee, Scientific Directors, and Office of Intramural Training and Education and is funded by the Scientific Directors. FARE awards are based on scientific merit, originality, experimental design, and overall quality and presentation of the abstracts.
Members of the Hu Lab who were FARE awardees in previous years include Alice Duchon and Chijioke Umunnakwe (2020), Jonathan Rawson (2019), Sheikh Abdul Rahman (2016), Luca Sardo (2015), Kari Dilley (2012), Michael Moore (2009), Mario P.-S. Chin (2006 and 2007), Kazushi Motomura (2007), and Olga Nikolaitchik (2006).
Wei-Shau Hu Elected to American Academy of Microbiology
Wei-Shau Hu was elected as a Fellow in the American Society of Microbiology (AAM) in February 2021. The AAM is the honorific leadership group within the American Society for Microbiology, the world’s largest society dedicated to advancing the microbial sciences. AAM Fellows are recognized as distinguished researchers who are "elected through a highly selective, annual, peer review process, based on their records of scientific achievement and original contributions that have advanced microbiology....Each elected Fellow has built an exemplary career in basic and applied research, teaching, clinical and public health, industry or government service."
PNAS Cozzarelli Prize Awarded to Pathak and Hu Labs
A recent study published by the research groups of Vinay Pathak and Wei-Shau Hu in Proceedings of the National Academy of Sciences USA (“HIV-1 uncoats in the nucleus near sites of integration,” PNAS 117: 5486-5493, 2020) was awarded the 2020 Cozzarelli Prize in the class of Biomedical Sciences. The Cozzarelli Prize is awarded to just 6 papers chosen from nearly 4,000 published research articles, one in each of the 6 classes of the National Academy of Sciences. They represent the top scientific research published in PNAS in 2020. To read more about this award, click here.
Uncoating of an infectious HIV-1 complex is shown at left. Click on the figure for a full caption and here to download the original video (Movie S1 in the paper).
This study was also featured on the website of the Center for Cancer Research, National Cancer Institute (“New study overturns conventional understanding of how HIV infection occurs”) and highlighted on the front cover of the Cold Spring Harbor 2020 Retroviruses Meeting abstract book.
Research by Pathak and Hu Labs Featured on Cover of 2020 Retroviruses Meeting Abstract Book
A recent study published by the research groups of Vinay Pathak and Wei-Shau Hu in Proceedings of the National Academy of Sciences USA (“HIV-1 uncoats in the nucleus near sites of integration,” PNAS 117:5486-5493, 2020) was featured on the front cover of the Cold Spring Harbor Laboratory 2020 Retroviruses Meeting abstract book. Pathak lab member Ryan Burdick launched the meeting with his talk on the study and reported that HIV-1 uncoats in the nucleus near sites of integration. Contrary to the prevailing theory for more than 40 years that retroviral uncoating occurs in the cytoplasm, the study team showed that HIV-1 cores are essentially intact as they enter the nucleus, where they complete reverse transcription before uncoating near their sites of integration into the host genome. These unexpected results fundamentally alter the current understanding of HIV-1 replication, which could lead to the development of more effective strategies and drugs for the treatment of HIV infections.
In the cover image, the left panel shows an HIV-1 capsid localized in the nucleus and the right panel shows a site of transcription of the viral genome at the site where the capsid localized. To read more about the study, see the original research article and the commentary "Entering and breaking for HIV?" in Nature Reviews Microbiology.
This study was also awarded the 2020 PNAS Cozzarelli Prize in Biomedical Sciences and featured on the website of the Center for Cancer Research, National Cancer Institute (“New study overturns conventional understanding of how HIV infection occurs”).
CCR News Feature about HIV-1 Infection Study by Pathak and Hu Labs
[Excerpted from a 26 February 2020 news feature by Center for Cancer Research (CCR), National Cancer Institute]
New Study Overturns Conventional Understanding of How HIV Infection Occurs
With unprecedented detail, researchers have observed where and when the protective casing around the human immunodeficiency virus (HIV-1) is shed inside a human cell, which is an observation that overturns the conventional understanding of how the virus infects cells and replicates. The finding was published February 24, 2020, in the Proceedings of the National Academy of Sciences (PNAS) USA.
“Understanding how HIV-1 replication occurs can provide valuable insights into essential interactions between the virus and the host cell,” explains Vinay K. Pathak, Ph.D., Senior Investigator in the Retroviral Replication Laboratory, part of the HIV Dynamics and Replication Program.
Despite the importance of understanding how the virus infects human cells, many details about this process have remained unknown. Until now, many scientists suspected that the protective casing around the virus, called the viral capsid, was shed before the virus enters the center of a human cell, called the nucleus. Once inside the nucleus, the virus begins to replicate itself.
Pathak’s lab used a new approach to brightly label the viral capsid with fluorescence in order to track it. Previous efforts to label the viral capsid with fluorescent proteins resulted in dimly labeled or defective viruses, making it difficult to study the uncoating process.
Unexpectedly, they found that the viral capsid remained essentially intact after entering the nuclei of cells. They also identified a key protein called CPSF6 that helps facilitate the transport of the virus, including its capsid, through the pores of a nucleus.
“We hope that the insights gained in our studies will help to identify critical molecular interactions between the virus and the host that will facilitate the development of new classes of potent anti-retroviral drugs,” says Pathak.
However, he notes, this study raises many unanswered questions. It’s still a mystery, for example, on how the full virus (capsid included) at a diameter of roughly 61 nanometers can squeeze through the pore of a human nucleus, which is only about 39 nanometers wide. More research is needed to understand how this happens and to identify the mechanisms that help trigger the virus’s uncoating process.
Confocal image of an infected cell nucleus (purple) shows an HIV-1 capsid core (green spot, left panel) just before uncoating and a transcriptionally active provirus (bright purple spot, right panel) that appears several hours later near the site of uncoating. Image credit: Ryan C. Burdick
[This study was also awarded the 2020 PNAS Cozzarelli Prize in Biomedical Sciences and highlighted on the front cover of the Cold Spring Harbor 2020 Retroviruses Meeting abstract book.]
Intramural AIDS Research Fellowship Awards
Intramural AIDS Research Fellowship (IARF) awards from the Office of AIDS Research, Office of Intramural Research, and Office of Intramural Research & Training in the National Institutes of Health include full stipend support to successful candidates who demonstrate outstanding scientific potential through both an imaginative and thoughtful research plan and a well thought out career development plan.
Alice Duchon received an IARF Award in 2019 and 2020 to support her research project on "Understanding the Interplay between HIV-1 Gag and the Viral RNA Genome that Facilitates Virus Assembly." Jonathan Rawson received an IARF Award in 2017 and 2018 to support his research project on "Understanding the Pseudodiploid Nature of HIV-1."
NIH Summer Mentor Award
Jonathan Rawson received a 2018 NIH Summer Mentor Award. The NIH Summer Mentor Award Program provides for a centrally funded summer intern to train with qualified postdoctoral fellows, visiting fellows, clinical fellows, and graduate students. Dr. Rawson's trainee is Franck Mbuntcha Bogni, a student who is participating in the NIH Community College Summer Enrichment Program this year.
Travel Award, HIV DRP Think Tank Meeting
Yang Liu received a travel award for one of the two best presentations by NCI fellows at the 2015 HIV DRP Think Tank Meeting. The $1000 travel award was provided by the HIV DRP, Center for Cancer Research, NCI.
Award from U.S.-Russia Joint Working Group on Biomedical Research Cooperation
In 2012, Wei-Shau Hu was the recipient of one of the five grants that the U.S.-Russia Joint Working Group on Biomedical Research Cooperation awarded to National Cancer Institute intramural investigators for their highly meritorious research applications. Dr. Hu is the Principal Investigator on a project focused on understanding the impact of HIV-1 recombination and cell-to-cell transmission on vaccine development and chemoprevention strategy.
Marie Curie Postdoctoral Fellowship
Andrea Galli was awarded a Marie Curie Postdoctoral Fellowship in 2011 by Copenhagen University.
Howard Temin Pathway to Independence Award (K99/R00)
In 2008, Mario P.-S. Chin successfully competed for a Howard Temin Pathway to Independence (PI) Award (K99/R00) from the National Institutes of Health. The PI Award Program establishes and maintains a strong cohort of new and talented NIH-supported independent investigators. Subsequent to receiving this award, Dr. Chin accepted a position at the Aaron Diamond AIDS Research Center (ADARC) in New York as the first ADARC Scholar. At the ADARC, he has established an independent research program that is focused on the evolution and adaption of HIV-1 in response to antiviral drug and host immune selection pressures.
Kitazato Shibasaburo Award
In 2008, Kazushi Motomura won the Kitazato Shibasaburo Award in recognition of the important findings from a study on HIV-1 and HIV-2 recombination that he reported with Jianbo Chen and Wei-Shau Hu (J. Virol. 82: 1923-1933, 2008). This award is one of the most prestigious prizes in the infectious disease field in Japan.
Scholarship Award, Keystone Symposia on HIV Pathogenesis
Michael Moore was awarded a travel scholarship to present his research findings at the 2008 Keystone Symposia on HIV Pathogenesis.
Scholarship Award, International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention
Mario P.-S. Chin was awarded a scholarship to present his findings at the 2007 IAS Conference on HIV Pathogenesis, Treatment and Prevention in Sydney, Australia.
Poster Awards, Spring Research Festival at NCI-Frederick
Michael Moore and Olga Nikolaitchik won poster awards for their presentations at the NCI-Frederick Spring Research Festival in 2007 and 2006, respectively.