Laboratory of Genome Integrity

Chief
Andre Nussenzweig, Ph.D.

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

 

                                         The Laboratory of Genome Integrity, July 2019

                                               

Position Keywords Contact Name E-mail Address
Post-doctoral Fellow - DNA damage, DNA repair, computational biology, genomics DNA damage, DNA repair, computational biology, genomics Sam John sam.john@nih.gov

LGI Flow Cytometry Core Facility - NIH 37 logo

Flow Cytometry Core

The Flow Cytometry Core in Building 37, overseen by the Laboratory of Genome Integrity (LGI), is under the supervision of Ferenc Livak, MD. Flow Core 37 supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry analysis and cell sorting. Currently, the laboratory houses:
  • 4 cell analyzers: 1 FACS Calibur, 1 FACSCanto, and 2 LSRII Fortessa (up to 18-color analysis)
  • 4 cell sorters:  2 BD FACS Arias, 1 MoFlo Astrios EQ, and 1 BD FACSAria Fusion

The analytical instruments are available to trained users on a 24/7 basis. Cell sorting is mostly performed by staff.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry, please go to https://labshare.nih.gov/nci/CCR-FACS/SitePages/Home.aspx (link is external)

   

Andre Nussenzweig - Wei Yang Virtual Seminar Series
Time and Link (unless otherwise noted):
 
For talks on Aug 17 and beyond use the below Zoom link
Time: 11AM EDT
Meeting ID: 981 3923 4399
Password: 785759
 
Mon 3-Aug
No speaker scheduled
 
For Aug 10 use the below Zoom link
Time: 11AM EDT 
 
Mon 10-Aug
Speaker: Thomas Kunkel, DNA Replication Fidelity Group, Genome Integrity & Structural Biology Lab, NIEHS, NIH, Research Triangle Park, NC
Title: Recent progress in understanding eukaryotic DNA replication fidelity
 
Mon 17-Aug
Speaker: Bo-Ruei Chen​, Division of Hematology and Oncology, Department of Medicine, University of Alabama at Birmingham,  Birmingham, AL 
Title: The DREAM complex prevents DNA end resection and initiation of HR in G0/G1 phase of the cell cycle
 
Mon 24-Aug
Speaker: Scott Williams​, Structural Cell Biology Group, Deputy Chief, Genome Integrity & Structural Biology Lab, NIEHS, NIH, Research Triangle Park, NC
Title: Resolution of Topoisomerase DNA-protein crosslinks
 
Mon 31-Aug
Speaker: Karen Usdin​, Gene Structure and Disease Section, Deputy Chief, Laboratory of Cell and Molecular Biology, NIDDK, NIH, Bethesda, MD
Title: The Dark Side of DNA Repair: The Mechanism of Repeat Expansion Responsible for the Fragile X-related Disorders
 
Mon 21-Sept 
Speaker: Kai Ge, Senior Investigator, Adipocyte Biology and Gene Regulation Section, Laboratory of Endocrinology and Receptor Biology, NIDDK, NIH, Bethesda, MD
Title: 'TBA'
 
Mon 28-Sept 
Speaker: Núria López-Bigas, Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Spain
Title: Mutational footprints of cancer therapies 
 
Tue 6-Oct ** Please note that this is a Tuesday seminar starting at 11AM EDT 
Speaker: Jim Haber, Professor of Biology and Director, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA
Title: Fidelity and infidelity of DNA repair 
 
Mon 19-Oct
Speaker: Susan Rosenberg, Departments of Molecular & Human Genetics, Biochemistry & Molecular Biology & Molecular Virology & Microbiology, Baylor College of Medicine, Houston, TX 
Title: 'TBA' 
 
Mon 26-Oct
Speaker: Keji Zhao, Center Director Systems Biology Center, Laboratory of Epigenome Biology, National Heart, Lung and Blood Institute, NIH, Bethesda, MD 
Title: 'TBA' 
 
Mon 2-Nov
Speaker: Joseph Loparo, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA  
Title: 'TBA 
 
Mon 9-Nov
Speaker: Steve Jackson, Head of Cancer Research Laboratories UK at the Wellcome/Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, UK
Title: 'TBA' 
 
Mon 16-Nov
Speaker: Xiaolan Zhao, Molecular Biology Department, Memorial Sloan Kettering Cancer Center, New York, NY​ 
Title: 'TBA 
 
Wed 2-Dec ** Please note that this is a Wednesday seminar starting at 11AM EDT 
Speaker: Eric Greene, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY
Title: 'TBA 
 
Mon 14-Dec
Speaker: David Pellman, Department of Cell Biology, Harvard Medical School and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA    
Title: 'TBA'
 
Mon 21-Dec
Speaker: David Cortez, Professor of Biochemistry, Associate Director for Basic Science Research at Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical School, Nashville, TN  
Title:  'TBA'
 
Mon 28-Dec
Speaker: Guo-Min Li, Professor of Radiation Oncology and Director of the Reece A. Overcash Jr. Center for Research on Colon Cancer, University of Texas SouthWestern, Dallas, TX  
Title:  'TBA'
 
Mon 11-Jan
Speaker: Johannes Walter, Howard Hughes Medical Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 
Title:  'TBA'
-----------------------------------------------------------------------------------------------------------------------------------------------------
Past Seminars
Time: 11AM EST
Meeting ID: 981 3923 4399
Password: 785759
 
Mon 11-May 
Speaker: Dale Ramsden, Ph.D., Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC
Title: Promiscuous synthesis during repair of chromosome breaks: what goes in, when, and why  
 
Mon 18-May 
Speaker: Peter Cejka, Ph.D., Institute for Research in Biomedicine, Faculty of Biomedical Sciences, Switzerland  
Title: The function of nucleases in homologous recombination
 
Wed 20-May
Speaker: Alberto Martin, Ph.D., Department of Immunology, University of Toronto, Toronto, Canada
Title: DNA damage in immunity and cancer
 
Mon 8-June
Speaker: Tanya Paull, Ph.D., Professor, Department of Molecular Biosciences, University of Texas at Austin, TX 
Title: ATM loss and cerebellar neurodegeneration​
 
Mon 15-June
Speaker: Karl-Peter Hopfner, Ph.D., Department of Biochemistry and Gene Center, Ludwig-Zoom Maximilians-University, Munich, Germany
Title: Structural mechanism of DNA end sensing and processing by Mre11-Rad50
 
Mon 22-June
Speaker: Karlene Cimprich, Ph.D., Professor of Chemical and Systems Biology, Stanford University School of Medicine, CA 
Title: The Causes and Consequences of Replication Stress:  HLTF and Replication Stress Resistance
 
Mon 29-June
Speaker: Anna Malkova, Ph.D., Professor of Biology, Department of Biology, University of Iowa, Iowa City, Iowa 
Title: Break-Induced Replication - unusual mode of DNA synthesis leading to genomic instability
 
Mon 6-July
Speaker: Feilong Meng, Ph.D., Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China
Title: Repair of AID-initiated DNA lesions in B cells
 
Mon 13-July
Speaker: Shan Zha, M.D. Ph.D. Associate Professor, Department of Pediatrics, Institute for Cancer Genetics, Columbia University, New York, NY    
Title: Loss vs Inhibition – From DNA-PK to PARP1&2
 
Mon 20-July
Speaker: Catherine Freudenreich, Ph.D., Professor and Chair, Department of Biology; Member, Program in Genetics; Tufts University, MA
Title: The role of nuclear re-location in promoting restart of collapsed replication forks and preventing genome instability’​
 
Mon 27-July
Speaker: Keith Caldecott, Professor of Biochemistry and Deputy Director, Genome Damage and Stability Centre, University of Sussex, UK
Title: DNA Strand Breakage and Human Genetic Disease
 
 

About

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

 

                                         The Laboratory of Genome Integrity, July 2019

                                               

PI & Key Staff

Positions

Position Keywords Contact Name E-mail Address
Post-doctoral Fellow - DNA damage, DNA repair, computational biology, genomics DNA damage, DNA repair, computational biology, genomics Sam John sam.john@nih.gov

Cores

LGI Flow Cytometry Core Facility - NIH 37 logo

Flow Cytometry Core

The Flow Cytometry Core in Building 37, overseen by the Laboratory of Genome Integrity (LGI), is under the supervision of Ferenc Livak, MD. Flow Core 37 supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry analysis and cell sorting. Currently, the laboratory houses:
  • 4 cell analyzers: 1 FACS Calibur, 1 FACSCanto, and 2 LSRII Fortessa (up to 18-color analysis)
  • 4 cell sorters:  2 BD FACS Arias, 1 MoFlo Astrios EQ, and 1 BD FACSAria Fusion

The analytical instruments are available to trained users on a 24/7 basis. Cell sorting is mostly performed by staff.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry, please go to https://labshare.nih.gov/nci/CCR-FACS/SitePages/Home.aspx (link is external)

   

Contact Info

Laboratory of Genome Integrity
Center for Cancer Research
National Cancer Institute
Building 37, Room 1108
Bethesda, MD 20892-4254
Ph: 240-760-7607
Fax: 240-541-4489
Technical Lab Manager
240-760-7601
Administrative Assistant
240-760-6687
Administrative Assistant
240-760-6382