Laboratory of Genome Integrity

Chief
Andre Nussenzweig, Ph.D.

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

 

                                         The Laboratory of Genome Integrity, July 2019

                                               

Position Degree Required Contact Name E-mail Address
Post-doctoral Fellow - DNA damage, DNA repair, genomics Ph.D. or equivalent Sam John sam.john@nih.gov
Post-doctoral Fellow - DNA damage, DNA repair, computational biology, genomics Ph.D. or equivalent Sam John sam.john@nih.gov

LGI Flow Cytometry Core Facility - NIH 37 logo

Flow Cytometry Core

The Flow Cytometry Core in Building 37, overseen by the Laboratory of Genome Integrity (LGI), is under the supervision of Ferenc Livak, MD. Flow Core 37 supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry analysis and cell sorting. Currently, the laboratory houses:
  • 4 cell analyzers: 1 FACS Calibur, 1 FACSCanto, and 2 LSRII Fortessa (up to 18-color analysis)
  • 4 cell sorters:  2 BD FACS Arias, 1 MoFlo Astrios EQ, and 1 BD FACSAria Fusion

The analytical instruments are available to trained users on a 24/7 basis. Cell sorting is mostly performed by staff.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry, please go to https://labshare.nih.gov/nci/CCR-FACS/SitePages/Home.aspx (link is external)

   

Andre Nussenzweig - Wei Yang Virtual Seminar Series
Time and Link (unless otherwise noted):
 
Please use the below Zoom link
Time: 11AM EDT
Meeting ID: 161 822 3083
Password: 675056
 
Mon 26-Oct
Speaker: Keji Zhao, Center Director, Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung and Blood Institute, NIH, Bethesda, MD
Title: Epigenetic Basis of Cellular Heterogeneity
 
Mon 2-Nov
Speaker: Joseph Loparo, Associate Professor, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA  
Title: Holding it together while avoiding mistakes: mechanistic insights into non-homologous end joining​ 
 
Mon 9-Nov
Speaker: Michael Seidman, Chief, Section on Gene Targeting, Laboratory of Molecular Gerontology, National Institute of Aging, NIH, Baltimore, MD 
Title: Replisome remodeling following encounters with an absolute block
 
Mon 16-Nov
Speaker: Xiaolan Zhao, Molecular Biology Department, Memorial Sloan Kettering Cancer Center, New York, NY​ 
Title: 'TBA 
 
Wed 2-Dec ** Please note that this is a Wednesday seminar starting at 11AM EDT 
Speaker: Eric Greene, Professor, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY
Title: Single molecule studies of homologous recombination
 
Mon 14-Dec
Speaker: David Pellman, Department of Cell Biology, Harvard Medical School and Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA    
Title: 'TBA'
 
Mon 21-Dec
Speaker: David Cortez, Richard Armstrong Professor, Vanderbilt University School of Medicine, Department of Biochemistry, Nashville, TN 
Title: RADX regulates RAD51 at replication forks to maintain genome stability
 
Mon 28-Dec
Speaker: Guo-Min Li, Professor of Radiation Oncology and Director of the Reece A. Overcash Jr. Center for Research on Colon Cancer, University of Texas SouthWestern, Dallas, TX  
Title: DNA mismatch repair: mechanisms and therapeutic potential
 
Mon 4-Jan
Speaker: Michael Kosicki, Lawrence Berkeley National Laboratory, Berkeley, CA​ 
Title:  'TBA'
 
Mon 11-Jan
Speaker: Johannes Walter, Howard Hughes Medical Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 
Title:  'TBA'
 
Mon 25-Jan
Speaker: Andrew Jackson, MRC Human Genetics Institute, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, Scotland, UK
Title:  'TBA'
 
Mon 1-Feb
Speaker: Rodney Rothstein, Department of  Genetics & Development,  Columbia University,  New York, NY
Title:  'TBA'
 
Mon 8-Feb
Speaker: Dan Durocher, Senior Investigator, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, Ontario, Canada
Title:  Mining synthetic lethality networks for new DNA repair biology
 
Mon 22-Feb
Speaker: David Gilbert, Senior Investigator, San Diego Biomedical Research Institute (SDBRI), La Jolla, CA (as of Jan. 2021) - Former Professor, Department of Biological Science, Florida State University, Tallahassee, FL 
Title:  Regulation of Replication Timing and Chromosome Architecture
 
Mon 1-Mar
Speaker: Sue Jinks-RobertsonDepartment of Molecular Genetics and Microbiology and the University Program in Genetics and Genomics, Duke University, Durham, NC
Title:  'TBA'
 
Mon 8-Mar 
Speaker: Jim Haber, Professor of Biology and Director, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA
Title: Fidelity and infidelity of DNA repair 
 
Mon 15-Mar
Speaker: Agnel Sfeir, Skirball Associate Professor of Genetics, Skirball Institute/NYU School of Medicine, New York, NY
Title:  'TBA'
 
Mon 22-Mar
Speaker: Pierre-Henri Gaillard, Group Leader at Cancer Research Centre of Marseille, Marseille, France
Title:  'TBA'
 
Mon 29-Mar
Speaker: Zhiguo Zhang, Deaprtment of Pediatric and Genetics and Development, Columbia University, New York, NY
Title:  'TBA'
 
Mon 5-Apr
Speaker: Roger Greenberg, Department of Cancer Biology, The Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA
Title:  Chromatin directed mechanisms of DNA repair
 
Mon 12-Apr
Speaker: Sophie Polo, Junior Group Leader, Epigenetics & Cell Fate Centre, University of Paris, Paris, France
Title:  Epigenome maintenance in response to DNA damage
 
Mon 19-Apr
Speaker: Xin Chen, Department of Biology, Johns Hopkins University, Baltimore, MD
Title:  'TBA'
 
Mon 26-Apr
Speaker: Helle Ulrich, Scientific Director, Institute of Molecular Biology (IMB), Professor, Faculty of Biology, Johannes Gutenberg University, Mainz, Germany
Title: Dealing with DNA damage during replication
 
Mon 3-May
Speaker: Gaelle Legube, Laboratory of Cellular and Molecular Biology Control Proliferation, The Centre for Integrative Biology, CNRS, Université de Toulouse, Toulouse, France
Title:  'TBA'
 
Mon 10-May
Speaker: Titia Sixma, Group Leader, Biochemistry Division, Netherlands Cancer Institute, Amsterdam, Netherlands
Title:  'TBA'
 
Mon 17-May
Speaker: Steve Jackson, Professor, Head of Cancer Research UK Laboratories, The Gurdon Institute, University of Cambridge, Cambridge, UK
Title: Cellular responses to DNA double-strand breaks: mechanistic insights and clinical applications
 
Mon 14-June
Speaker: Jean Gautier, Department of Genetics and Development, Institute for Cancer Genetics, Columbia University, New York, NY
Title:  'TBA'
 
Mon 21-June
Speaker: Ludovic Deriano, Head of the Genome Integrity, Immunity and Cancer Unit, Department of Immunology, Department of Genomes and Genetics, Institut Pasteur, Paris, France 
Title:  'TBA'
 
-----------------------------------------------------------------------------------------------------------------------------------------------------
Past Seminars
Time: 11AM EST
Meeting ID: 981 3923 4399
Password: 785759
 
Mon 11-May 
Speaker: Dale Ramsden, Ph.D., Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC
Title: Promiscuous synthesis during repair of chromosome breaks: what goes in, when, and why  
 
Mon 18-May 
Speaker: Peter Cejka, Ph.D., Institute for Research in Biomedicine, Faculty of Biomedical Sciences, Switzerland  
Title: The function of nucleases in homologous recombination
 
Wed 20-May
Speaker: Alberto Martin, Ph.D., Department of Immunology, University of Toronto, Toronto, Canada
Title: DNA damage in immunity and cancer
 
Mon 8-June
Speaker: Tanya Paull, Ph.D., Professor, Department of Molecular Biosciences, University of Texas at Austin, TX 
Title: ATM loss and cerebellar neurodegeneration​
 
Mon 15-June
Speaker: Karl-Peter Hopfner, Ph.D., Department of Biochemistry and Gene Center, Ludwig-Zoom Maximilians-University, Munich, Germany
Title: Structural mechanism of DNA end sensing and processing by Mre11-Rad50
 
Mon 22-June
Speaker: Karlene Cimprich, Ph.D., Professor of Chemical and Systems Biology, Stanford University School of Medicine, CA 
Title: The Causes and Consequences of Replication Stress:  HLTF and Replication Stress Resistance
 
Mon 29-June
Speaker: Anna Malkova, Ph.D., Professor of Biology, Department of Biology, University of Iowa, Iowa City, Iowa 
Title: Break-Induced Replication - unusual mode of DNA synthesis leading to genomic instability
 
Mon 6-July
Speaker: Feilong Meng, Ph.D., Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China
Title: Repair of AID-initiated DNA lesions in B cells
 
Mon 13-July
Speaker: Shan Zha, M.D. Ph.D. Associate Professor, Department of Pediatrics, Institute for Cancer Genetics, Columbia University, New York, NY    
Title: Loss vs Inhibition – From DNA-PK to PARP1&2
 
Mon 20-July
Speaker: Catherine Freudenreich, Ph.D., Professor and Chair, Department of Biology; Member, Program in Genetics; Tufts University, MA
Title: The role of nuclear re-location in promoting restart of collapsed replication forks and preventing genome instability’​
 
Mon 27-July
Speaker: Keith Caldecott, Professor of Biochemistry and Deputy Director, Genome Damage and Stability Centre, University of Sussex, UK
Title: DNA Strand Breakage and Human Genetic Disease
 
Mon 3-Aug
No speaker scheduled
 
Mon 10-Aug
Speaker: Thomas Kunkel, DNA Replication Fidelity Group, Genome Integrity & Structural Biology Lab, NIEHS, NIH, Research Triangle Park, NC
Title: Recent progress in understanding eukaryotic DNA replication fidelity
 
Mon 17-Aug
Speaker: Bo-Ruei Chen​, Division of Hematology and Oncology, Department of Medicine, University of Alabama at Birmingham,  Birmingham, AL 
Title: The DREAM complex prevents DNA end resection and initiation of HR in G0/G1 phase of the cell cycle
 
Mon 24-Aug
Speaker: Scott Williams, Head - Structural Cell Biology Group, Deputy Chief - Genome Integrity & Structural Biology Laboratory, NIEHS, NIH, North Carolina
Title: Resolution of Topoisomerase DNA-protein crosslinks
 
Mon 31-Aug
Speaker: Karen Usdin​, Gene Structure and Disease Section, Deputy Chief, Laboratory of Cell and Molecular Biology, NIDDK, NIH, Bethesda, MD
Title: The Dark Side of DNA Repair: The Mechanism of Repeat Expansion Responsible for the Fragile X-related Disorders
 
Mon 7-Sept 
No speaker scheduled
 
Wed 9-Sept ** Please note that this is a Wednesday seminar starting at 11AM EDT ** 
Speaker: Sergei Mirkin, White Family Chair in Biology, Department of Biology, Tufts University, Medford, MA  
Title: 'Genome Instability Mediated by Trinucleotide Repeats In Dividing and Non-dividing Cells'
 
Mon 14-Sept
No speaker scheduled
 
Mon 21-Sept 
Speaker: Kai Ge, Ph.D., Senior Investigator and Section Chief, Adipocyte Biology & Gene Regulation Section, NIDDK, NIH, Bethesda, MD
Title: Epigenomic Regulation of Enhancers: from Adipogenesis, Development to Cancer
 
Mon 28-Sept 
Speaker: Núria López-Bigas, Ph.D., ICREA Research Professor, Barcelona Biomedical Genomics Lab, Institute for Research in Biomedicine (IRB), Barcelona, Spain 
Title: Computational analysis of thousands of cancer genomes to understand mutational processes
 
Mon 5-Oct
No speaker scheduled
 
Mon 12-Oct
No speaker scheduled
 
Mon 19-Oct
Speaker: Susan Rosenberg, Professor, Departments of Molecular & Human Genetics, Biochemistry & Molecular Biology & Molecular Virology & Microbiology, Baylor College of Medicine, Houston, TX 
Title: DNA Reaction Intermediates in Living Cells 
 

About

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

 

                                         The Laboratory of Genome Integrity, July 2019

                                               

PI & Key Staff

Positions

Position Degree Required Contact Name E-mail Address
Post-doctoral Fellow - DNA damage, DNA repair, genomics Ph.D. or equivalent Sam John sam.john@nih.gov
Post-doctoral Fellow - DNA damage, DNA repair, computational biology, genomics Ph.D. or equivalent Sam John sam.john@nih.gov

Cores

LGI Flow Cytometry Core Facility - NIH 37 logo

Flow Cytometry Core

The Flow Cytometry Core in Building 37, overseen by the Laboratory of Genome Integrity (LGI), is under the supervision of Ferenc Livak, MD. Flow Core 37 supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry analysis and cell sorting. Currently, the laboratory houses:
  • 4 cell analyzers: 1 FACS Calibur, 1 FACSCanto, and 2 LSRII Fortessa (up to 18-color analysis)
  • 4 cell sorters:  2 BD FACS Arias, 1 MoFlo Astrios EQ, and 1 BD FACSAria Fusion

The analytical instruments are available to trained users on a 24/7 basis. Cell sorting is mostly performed by staff.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry, please go to https://labshare.nih.gov/nci/CCR-FACS/SitePages/Home.aspx (link is external)

   

Contact Info

Laboratory of Genome Integrity
Center for Cancer Research
National Cancer Institute
Building 37, Room 1108
Bethesda, MD 20892-4254
Ph: 240-760-7607
Fax: 240-541-4489
Technical Lab Manager
240-760-7601
Administrative Assistant
240-760-6687
Administrative Assistant
240-760-6382