Laboratory of Genome Integrity
Laboratory of Genome Integrity
About
The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.
The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.
Job Vacancies
| Position | Degree Required | Contact Name | Contact Email |
|---|---|---|---|
| Postdoctoral Fellow - DNA damage, DNA repair | Ph.D. or equivalent | Sam John | sam.john@nih.gov |
| Postdoctoral Fellow - Neuronal development and disorders, Chromatin regulation | Ph.D. or equivalent | Lisa Boxer | lisa.boxer@nih.gov |
| Biologist - Neurodevelopmental disorders, cancer | B.S./B.A. or equivalent | Lisa Boxer | lisa.boxer@nih.gov |
News
Cores
Flow Cytometry Core
The Flow Cytometry Core in Building 37, overseen by the Laboratory of Genome Integrity (LGI), is under the supervision of Ferenc Livak, M.D. Flow Core 37 supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry analysis and cell sorting. Currently, the laboratory houses:
- 5 cell analyzers: FACSCanto (8-color), LSRII Fortessa SORP 1 (18-color), LSRII Fortessa SORP 2 (18-color) FACSymphony A5 (25-color), Sony ID700 spectral analyzer (30-color)
- 1 imaging flow analyzer: Amnis ImageStream MarkII (2-camera/10-color)
- 4 cell sorters: BD FACS Aria IIu (10-color), FACSAria IIu (11-color), BD FACSAria Fusion (18-color), Sony MA900 (9-color)
The analytical instruments are available to trained users on a 24/7 basis. Cell sorting is mostly performed by staff.
These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).
For information on accessing the LGI Flow Cytometry Core, go to https://nci.corefacilities.org/service_center/show_external/5939
Seminars
Laboratory of Genome Integrity Seminar Series
This series is sponsored by the Principal Investigators of the Laboratory of Genome Integrity (LGI). Unless otherwise stated, all seminars will be held at 11:30AM in the 4th floor conference room of Building 37 on the Bethesda NIH Campus. For more information, please contact Sam John at sam.john@nih.gov
Visit our archives to learn more about past seminars
Upcoming Seminars: 2024
Wed 28-Aug
Title: Proteogenomic insights into multiple myeloma
Speaker: Ryan Young, Stadtman Investigator, Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Wed 30-Oct
Title: Mis-regulation of Chromosome dynamics as a Reproductive-isolating barrier in mice
Speaker: Takashi Akera, Stadtman Investigator, Laboratory of Chromosome dynamics and Evolution, Cell and Developmental Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD
Tue 12-Nov (Note: this seminar is at 11:30AM in the 2nd floor conference room of Building 37)
Title: TBA
Speaker: Katrin Mayer-Barber, Stadtman Investigator, Chief, Inflammation & Innate Immunity Unit, Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD
Wed 20-Nov
Title: TBA
Speaker: Yuichi Machida, Senior Investigator, Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Wed 11-Dec
Title: Untangling enhancer-promoter interactions throughout development
Speaker: Pedro Rocha, Stadtman Investigator, Unit on Genome Structure and Regulation, National Institute of Child Health and Development and Adjunct Investigator, Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Upcoming Seminars: 2025
Wed 15-Jan
Title: From axon damage to disease: common pathways in neurodegeneration
Speaker: Claire Le Pichon, Senior Investigator, Unit on the Development of Neurodegeneration, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD
Wed 29-Jan
Title: TBA
Speaker: Mark Histed, Chief, Unit on Neural Computation Behavior, National Institute of Mental Health, National Institutes of Health, Bethesda, MD
Wed 12-Feb
Title: TBA
Speaker: Todd Macfarlan, Senior Investigator, Section on Mammalian Epigenomic Reprogramming, National Institute of Child Health and Development , National Institutes of Health, Bethesda, MD
Wed 26-Feb
Title: TBA
Speaker: Keji Zhao, Chief, Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD
Wed 12-Mar
Title: TBA
Speaker: Alexander Chesler, Senior Investigator, Section on Sensory Cells and Circuits, National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD
Wed 26-Mar
Title: TBA
Speaker: Efsun Arda, Stadtman Investigator, Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Wed 9-Apr
Title: Molecular mechanisms underlying cell cycle dynamics and cell fate decisions
Speaker: Steven Cappell, Stadtman Investigator, Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Wed 23-Apr
Title: TBA
Speaker: Eytan Ruppin, Chief, Cancer Data Science Laboratory, Head, Computational Precision Oncology Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD
Wed 21-May
Title: TBA
Speaker: Thomas Gonatopoulos-Pournatzis, Stadtman Investigator, RNA Biology Laboratory, Head, Functional Transcriptomics Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD
