Word Cloud for Laboratory of Genome Integrity: instability, cancer, aging, heredity, disease, pathways, DNA, repair, development, understanding, screening, strategies, therapy, targeting, selectivity, maintenance, application, approaches, cells, pathology.
Chief
Andre Nussenzweig, Ph.D.
Staff Scientist
Elsa Callen Moreu, Ph.D.
Technical Laboratory Manager
Sam John, Ph.D.

Center for Cancer Research
National Cancer Institute

Building 37, Room 1108
Bethesda, MD 20892-4254
301-435-6425

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

Position Contact Name Contact E-mail Contact Phone Research Area Keywords Number of Positions
Postdoctoral Fellow Avinash Bhandoola

avinash.bhandoola@nih.gov

301-435-9381

T cell development, regeneration, aging, chromatin structure

1
Postdoctoral Fellow Andre Nussenzweig

andre_nussenzweig@nih.gov

301-435-6425

DNA repair, aging, chromatin structure

2

The Flow Cytometry Core in Building 37 is overseen by the Laboratory of Genome Integrity (LGI). This core, under the supervision of Karen Wolcott, supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry experiments. Currently, the laboratory houses:

  • 4 cell analyzers:  2 FACS Caliburs, 1 LSRII, and 1 LSRII Fortessa
  • 4 cell sorters:  2 FACS Arias, 1 MoFlo Astrios EQ, and 1 Becton Dickinson FACSAria Fusion

The LSRII, LSRII Fortessa, and the 2 FACS Caliburs are available to trained users on a 24/7 basis.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry Core, visit the CCR Office of Science and Technology Resources.

About

The research program in the Laboratory of Genome Integrity is focused on the exploration of the causes and effects of genomic instability, mechanisms of DNA repair and the study of DNA repair breakdown as an initiating or protective event in aging and cancers. The program will emphasize a mechanistic understanding of the pathways that maintain genomic integrity, the intersection of these pathways with normal cellular physiology and cancer and the application of these insights to the development of new therapeutic strategies. The laboratory has made major contributions towards a detailed understanding of DNA repair pathway selection as a primary influence on genomic stability and drug resistance/sensitivity in breast and ovarian cancers and the influential role of DNA repair proteins in the promotion of specific hematological malignancies.

The Laboratory is also expanding its efforts in the areas of cellular identity and development by examining the differentiation of hematopoietic stem cells into early T-cell lineage progenitors. This new program will place particular importance towards a comprehensive understanding of the cellular signals that influence hematopoietic progenitor migration, the transcription factors that positively and negatively impact T-cell lineage selection and the contribution of deregulated normal developmental processes in T-cell aging and cancer. The Laboratory of Genome Integrity also manages a state-of-the-art flow cytometry core that houses four cell sorters, four cell analyzers and serves the scientific needs of over 200 scientists and 80 different principal investigators every year. This facility will continue to provide both routine as well as highly specialized sorting services to members of the CCR-NCI community.

Directory

Positions

Position Contact Name Contact E-mail Contact Phone Research Area Keywords Number of Positions
Postdoctoral Fellow Avinash Bhandoola

avinash.bhandoola@nih.gov

301-435-9381

T cell development, regeneration, aging, chromatin structure

1
Postdoctoral Fellow Andre Nussenzweig

andre_nussenzweig@nih.gov

301-435-6425

DNA repair, aging, chromatin structure

2

Cores

The Flow Cytometry Core in Building 37 is overseen by the Laboratory of Genome Integrity (LGI). This core, under the supervision of Karen Wolcott, supports the NCI-CCR scientific research community by providing training, instrumentation, and expertise to perform state-of-the art flow cytometry experiments. Currently, the laboratory houses:

  • 4 cell analyzers:  2 FACS Caliburs, 1 LSRII, and 1 LSRII Fortessa
  • 4 cell sorters:  2 FACS Arias, 1 MoFlo Astrios EQ, and 1 Becton Dickinson FACSAria Fusion

The LSRII, LSRII Fortessa, and the 2 FACS Caliburs are available to trained users on a 24/7 basis.

These instruments provide reliable and consistent service to investigators from over 35 NCI laboratories and branches (>80 principal investigators, 200 other investigators).

For information on accessing the LGI Flow Cytometry Core, visit the CCR Office of Science and Technology Resources.