Eugene Valkov

Eugene Valkov, D.Phil.

  • Center for Cancer Research
  • National Cancer Institute
RNA Biology Laboratory


Eugene Valkov's research group investigates molecular mechanisms that regulate messenger RNA stability. Our team developed procedures for the recombinant production and biochemical characterization of many human factors that have critical roles in the late stages of gene expression. Our approach is to study reconstituted systems through biochemistry and biophysical analysis to derive working models of their interactions and mechanisms of action. Collaborating with colleagues at the Center for Structural Biology, we utilize cryogenic electron microscopy with single-particle analysis to obtain a high-resolution structural view of the systems under study to understand the molecular mechanism. Our work has broad implications beyond pure research. Recently, we have begun to apply our knowledge and access to a unique panel of biological reagents to improve and inform the development of the next generation of RNA-based therapeutics to target cancer and other diseases.

Areas of Expertise

RNA Decay and Surveillance Pathways
RNA-Binding Proteins
Cryoelectron Microscopy
Molecular Interactions
RNA Therapeutics


Selected Recent Publications

Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation

Raisch, T., Chang, C-T., Levdansky, Y., Muthukumar, S., Raunser, S., & Valkov, E.
Nature Communications. 10: 3173, 2019.
Full-Text Article
[ Journal Article ]

Regulation of the multisubunit CCR4-NOT deadenylase in the initiation of mRNA degradation

Raisch, T. & Valkov, E.
Current Opinion in Structural Biology. 77: 102460, 2022.
Full-Text Article
[ Journal Article ]

RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation

Poetz, F., Corbo, J., Levdansky, Y., Spiegelhalter, A., Lindner, D., Magg, V., Lebedeva, S., Schweiggert, J., Schott, J., Valkov, E., & Stoecklin, G.
Nature Communications. 12: 7175, 2021.
Full-Text Article
[ Journal Article ]

The nexus between RNA-binding proteins and their effectors

He, S., Valkov, E., Cheloufi, S., & Murn, J.
Nature Reviews Genetics. 2022.
Full-Text Article
[ Journal Article ]

Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome

Enwerem, I.I.I., Elrod, N.D., Chang, C-T., Lin, A., Ji, P., Bohn, J.A., Levdansky, Y., Wagner, E.J., Valkov, E., & Goldstrohm, A.C.
RNA. 27: 445-464, 2021.
Full-Text Article
[ Journal Article ]


03/2023 - Eugene gave an invited talk at the University of California at Riverside.

03/2023 - Yevgen was among the two students recognized this year with the Center for Cancer Research Outstanding Ph.D. Student Award

12/2022 - Yevgen defended his Ph.D. thesis virtually at the University of Tuebingen, Germany, with an overall distinction of Magna Cum Laude.

12/2022 - Eugene gave an invited lecture at the Ohio State University, Columbus, Ohio.

12/2022 - The Current Opinion in Structural Biology collection on Protein Nucleic Acid Interactions (2022), where Eugene served as a Guest Editor, is published.

10/2022 - Eugene gave an invited lecture at Moderna headquarters in Boston, Massachusetts.

07/2022 - Adrianna obtained a Cancer Research Training Award (CRTA) from the NCI Intramural Continuing Umbrella of Research Experiences (iCURE) program.

06/2022 - Eugene gave an invited talk and chaired a session at The Post-transcriptional Control of Gene Expression Conference on RNA Decay in Jupiter, Florida.

06/2022 - Eugene joined the Editorial Board of the Journal of Structural Biology and Journal of Structural Biology: X.

04/2022 - Josh accepted an offer to join the Ph.D. program at Harvard University.

11/2021 - Wataru was awarded a Postdoctoral Fellowship from the Japanese Society for the Promotion of Science as part of the Kaitoku-NIH program to support his research.