John 'Jay' Schneekloth Jr., Ph.D.

John 'Jay' Schneekloth Jr., Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
Chemical Biology Laboratory


My laboratory uses chemical biology approaches to identify and study small molecules that bind to RNA.  

Areas of Expertise

RNA-binding small molecules
Chemical Biology
Organic Chemistry


Selected Key Publications

Targeting Non-coding RNA Sensitizes Cancer Cells to Drugs

Yang M, Schneekloth JS Jr.
Trends Pharmacol Sci. 40(7): 447-8, 2019. [ Journal Article ]

Synthetic ligands for PreQ1 riboswitches provide structural and mechanistic insights into targeting RNA tertiary structure

Connelly CM, Numata T, Boer RE, Moon MH, Sinniah RS, Barchi JJ, Ferré-D'Amaré AR, Schneekloth JS Jr.
Nat Commun. 10(1): 1501, 2019. [ Journal Article ]

Targeting Mammalian Translational Inhibition with Tetracyclines

Boer RE, Schneekloth JS Jr.
Cell Chem Biol. 25(12): 1437-38, 2018. [ Journal Article ]

Chemical and structural studies provide a mechanistic basis for recognition of the MYC G-quadruplex

Calabrese DR, Chen X, Leon EC, Gaikwad SM, Phyo Z, Hewitt WM, Alden S, Hilimire TA, He F, Michalowski AM, Simmons JK, Saunders LB, Zhang S, Connors D, Walters KJ, Mock BA, Schneekloth JS Jr.
Nat Commun. 9(1): 4229, 2018. [ Journal Article ]

Characterization of clinically used oral antiseptics as quadruplex-binding ligands

Calabrese DR, Zlotkowski K, Alden S, Hewitt WM, Connelly CM, Wilson RM, Gaikwad S, Chen L, Guha R, Thomas CJ, Mock BA, Schneekloth JS Jr.
Nucleic Acids Res. 46(6): 2722-32, 2018. [ Journal Article ]

Job Vacancies

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Postdoctoral Fellow (Visiting)
Sumirtha Balaratnam, Ph.D.
Postdoctoral Fellow (CRTA)
Christopher Fullenkamp, Ph.D.
Postdoctoral Fellow (Visiting)
Shaifaly Parmar, Ph.D.
Postdoctoral Fellow (CRTA)
Christopher Sibley, Ph.D.
Postdoctoral Fellow (Visiting)
Mo Yang, Ph.D.
Peri Prestwood, B.S.


Parallel Discovery Strategies Provide a Basis for Riboswitch Ligand Design

Parallel Discovery Strategies Provide a Basis for Riboswitch Ligand Design

Published Date

The apple does not fall far from the tree. In this issue, Tran et al. describe synthetic small molecules that activate the ZTP riboswitch, a bacterial RNA that regulates gene expression. The apples signify RNA bound to particular synthetic ligands, with the best ligand being grasped by the researcher.


Parallel Discovery Strategies Provide a Basis for Riboswitch Ligand Design
Brandon Tran, Patricio Pichling, Logan Tenney, Colleen M. Connelly, Michelle H. Moon, Adrian R. Ferre´-D’Amare´ ,
John S. Schneekloth, Jr.* and Christopher P. Jones* in Cell Chemical Biology, 202027, 1241–1249