Eric O. Freed, Ph.D.
Dr. Freed is recognized as a leader in the field of virus assembly who has made important strides in understanding the mechanisms of retroviral replication at the molecular level, with an emphasis on late stages of the HIV-1 replication cycle. As Director of the HIV DRP, he oversees a program of basic, translational, and clinical research aimed at developing a better understanding of HIV that can be used to generate more-effective treatment strategies. His research focuses on HIV-1 Gag trafficking, Env incorporation, virus assembly, budding, release, and maturation. Dr. Freed has a special interest in the complex relationship between viral proteins and cellular factors and pathways, believing that characterizing fundamental aspects of the retrovirus life cycle will suggest novel targets for the development of antiretroviral therapies.
1) HIV pathogenesis, 2) virus assembly, 3) retroviruses, 4) virology, 5) cell biology,
6) structural biology
Assembly and Release of HIV-1 and Other Retroviruses
View Dr. Freed's Google Scholar page. Dr. Eric Freed - Complete Bibliography (April 2019)
Selected Key Publications
Biochemical evidence of a role for matrix trimerization in HIV-1 envelope glycoprotein incorporation.Proc Natl Acad Sci U S A . 113(2): E182-90, 2016. [ Journal Article ]
- PLoS Pathog. 8(11): Epub 2012 Nov 8, 2012. [ Journal Article ]
- Mol Cell. 30(2): 227-38, 2008. [ Journal Article ]
- Proc Natl Acad Sci U S A. 101(41): 14889-94, 2004. [ Journal Article ]
Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function.Proc Natl Acad Sci U S A. 99(2): 955-60, 2002. [ Journal Article ]
Dr. Eric Freed received his Ph.D. in 1990 in the laboratories of Drs. Rex Risser and Howard Temin at the University of Wisconsin-Madison and did postdoctoral work with Dr. Temin at UW-Madison in 1991. His work in Madison focused on the function of the murine leukemia virus and HIV envelope glycoproteins in membrane fusion and virus entry. He joined the Laboratory of Molecular Microbiology at the National Institute of Allergy and Infectious Diseases (LMM/NIAID) in 1992, where he worked with Dr. Malcolm Martin on HIV assembly and entry/post-entry events in the HIV replication cycle. In 1997 Dr. Freed was appointed as a Tenure-Track Investigator in LMM/NIAID, and he was promoted to a tenured Senior Investigator position in 2002. In 2003 he joined the HIV Drug Resistance Program (HIV DRP, renamed as the HIV Dynamics and Replication Program in 2015). He was an Organizer of the 2004 Cold Spring Harbor Meeting on Retroviruses, 2006 ASCB Conference "Cell Biology of HIV-1 and Other Retroviruses," 2012 Keystone Symposium "Frontiers in HIV Pathogenesis, Therapy and Eradication," 2014 Keystone Symposium "The Ins and Outs of Viral Infection: Entry, Assembly, Exit and Spread," Viruses 2016 Conference "At the Forefront of Virus–Host Interactions," and Viruses 2018 Conference "Breakthroughs in Virus Replication," and he served on the Scientific Committee of the International Retroviral Nucleocapsid Protein and Assembly Symposium in 2013 and 2016. He was appointed as the first Editor-in-Chief of Viruses in 2009, Editor of Journal of Molecular Biology in 2012, and Editor of Recent Advances in HIV-1 Assembly and Release in 2013. He also currently serves on the Editorial Boards of a number of journals, including Journal of Virology, Retrovirology, and Frontiers in Virology, and is an Associate Editor for Science Advances and Fields Virology. He was selected as an NCI Mentor of Merit in 2010 for excellence in mentoring and guiding the careers of trainees in cancer research, and in 2011 he was appointed to the NCI Senior Biomedical Research Service. Dr. Freed was appointed as the Deputy Director of the HIV DRP in 2014 and became Director of the HIV DRP in 2015. He was a sitting member of the NIH AIDS Discovery and Development of Therapeutics (ADDT) study section (2012-2017) and served as ADDT Chair from 2015 to 2017. He received the Outstanding Science Alumni Award from Penn State University in 2014 and the NCI Research Highlights Award in 2016. He is currently Chair of the Advisory Panel of the CCR Center for Molecular Microscopy, Co-Chair of the NIH Virology Interest Group, Chair of the 2018 Norman P. Salzman Memorial Symposium in Virology, and Co-Organizer of the gp41 Cytoplasmic Tail Structure and Function Workshop and is serving on the Organizing Committee of the 2018 Annual Meeting of the American Society for Virology. He is also a Co-Director of the University of Maryland Virology Program and an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park. In recognition of his outstanding contributions to the field of retrovirology, Dr. Freed was awarded the KT Jeang Retrovirology Prize in 2018. He was elected to Fellowship in the American Academy of Microbiology in 2019.
|Sherimay D. Ablan||Research Biologist|
|Melissa Fernandez Ph.D.||Postdoctoral Fellow (CRTA)|
|Yuta Hikichi Ph.D.||Postdoctoral Fellow (Visiting)|
|James Kirui Ph.D.||Postdoctoral Fellow (Visiting)|
|Alex B. Kleinpeter Ph.D.||Postdoctoral Fellow (CRTA)|
|Cheng Man (Bonnie) Lun Ph.D.||Postdoctoral Fellow (CRTA)|
|Ahlam Majadly||Summer Student (CRTA)|
|Lwar Naing||Postbaccalaureate Fellow (CRTA)|
|Phuong Pham||Postbaccalaureate Fellow (CRTA)|
|Nicole Powell||Werner H. Kirsten Student Intern (SIP)|
|Jennifer Simmons||Summer Student (CRTA)|
|Abdul A. Waheed, Ph.D.||Associate Scientist|
Pathway to Independence Award (K99/R00)
Melissa Fernandez successfully competed for a K99/R00 Pathway to Independence (PI) Award from the National Institutes of Health in 2019. The PI Award Program establishes and maintains a strong cohort of new and talented, NIH-supported, independent investigators. This program is designed to facilitate a timely transition of outstanding postdoctoral researchers or clinician-scientists from mentored research positions to independent, tenure-track or equivalent faculty positions, and to provide independent NIH research support during the transition that will help these individuals launch competitive, independent research careers.
Intramural AIDS Research Fellowships
Intramural AIDS Research Fellowship (IARF) awards from the Office of AIDS Research, Office of Intramural Research, and Office of Intramural Research & Training in the National Institutes of Health include full stipend support to successful candidates who demonstrate outstanding scientific potential through both an imaginative and thoughtful research plan and a well thought out career development plan.
Cheng man (Bonnie) Lun received an IARF award in 2019 to support her research project on "Mechanism of Viral Envelope Glycoprotein Targeting by Membrane-Associated RING-CH (MARCH) Proteins" and Melissa Fernandez received IARF awards in 2018 and 2019 to support her research project on "Mechanism of HIV-1 Env Trafficking to the Virological Synapse."
The following postdoctoral fellows in the Freed Lab received IARF awards in previous years:
Mariia Novikova: "Characterization of Antiretroviral Activity of Second-Generation Maturation Inhibitors and Mechanisms of Resistance" (2017)
Rachel Van Duyne: "Challenging the Paradigm for Antiretroviral Resistance through the Study of Non-Canonical HIV-1 Escape Mutants" (2017)
Emiko Urano: "Development of Potent and Broadly Active HIV-1 Maturation Inhibitors" (2016)
Mariia Novikova: "Mechanisms of HIV-1 Gag Lattice Formation and Env Incorporation" (2016)
Rachel Van Duyne: "Characterizing the Host Cell Factors Involved in HIV-1 Gag Trafficking to Sites of Virus Assembly" (2015)
Robert Buckheit: "The Effect of Host Antiviral Restriction Factors on Viral Budding and Maturation" (2014)
Lillian Kuo: "Characterizing the Role of HIV-1 p6-Alix Binding in HIV-1 Cell-to-Cell Infectivity" (2011, 2012)
Philip Tedbury: "HIV-1 Gag in Assembly and Release: Interactions with gp41 and Cellular Host Factors" (2011, 2012)
Poster Awards, Spring Research Festival
Ahlam Majadly was awarded "Outstanding Poster—Infectious Pathogens and Epidemiology" for her presentation at the 2019 Spring Research Festival, which is sponsored by the National Cancer Institute at Frederick and the other eight agencies of the National Interagency Confederation for Biological Research. Ahlam's presentation highlighted the research she conducted as a student trainee in the Freed Lab over the past year. Other members of the Freed Lab who won poster awards at the Spring Research Festival in previous years include Maya Swiderski (2016), Megan Mounts (2014), Scott MacDonald (2013), Darren D'Souza (2012), and Nishani Kuruppu (2011). All six of these award recipients were undergraduate students or Werner H. Kirsten student interns under the mentorship of Abdul Waheed.
Fellowship in the American Academy of Microbiology
Eric Freed was elected to Fellowship in the American Academy of Microbiology (AAM) in 2019. AAM Fellows are recognized as distinguished scientists who are "elected through a highly selective, annual, peer review process, based on their records of scientific achievement and original contributions that have advanced microbiology....Each elected Fellow has built an exemplary career in basic and applied research, teaching, clinical and public health, industry or government service."
New Investigator Scholarships, Conference on Retroviruses and Opportunistic Infections
Mariia Novikova, Phuong Pham, and Rachel Van Duyne were awarded New Investigator Scholarships to present their research findings at the 2019 Conference on Retroviruses and Opportunistic Infections (CROI). CROI scholarship awardees in previous years include Rachel Van Duyne (2018, 2017), Phuong Pham (2018), Mariia Novikova (2017, 2016), Justin Kaplan (2017), Emiko Urano (2016, 2014), Lillian Kuo (2013), and Catherine Adamson (2007).
2018 KT Jeang Retrovirology Prize
Eric Freed was awarded the 2018 KT Jeang Retrovirology Prize. This annual award recognizes mid-career investigators who have made outstanding contributions to the field of retrovirology.
NIH Postbac Poster Day Awards
Phuong Pham received an Outstanding Poster Award at the NIH Postbac Poster Day in 2018. Her presentation on "Progress in Developing Broadly Active and Highly Potent HIV-1 Maturation Inhibitors" was recognized as one of the best among the 772 posters presented by the NIH postbacs this year. Members of the Freed Lab who received Outstanding Poster Awards at the NIH Postbac Poster Day in previous years include Nishani Kuruppu and Justin Kaplan (2016).
Travel Awards, HIV DRP Think Tank Meeting
Rachel Van Duyne received a $1000 travel award from the HIV DRP for one of the two best presentations by NCI fellows at the 2018 and 2017 HIV DRP Think Tank Meetings. Members of the Freed Lab who received travel awards in previous years include Mariia Novikova (2016), Emiko Urano (2015), Kayoko Waki (2011), and Muthukumar Balasubramaniam (2010).
Travel Awards, Fall HIV/AIDS & Cancer Virology Think Tank Meeting
Mariia Novikova won a $1000 travel award for her outstanding talk at the 2017 Fall HIV/AIDS & Cancer Virology Think Tank Meeting. This annual Think Tank meeting on the NIH-Bethesda campus provides a venue for students, postdoctoral fellows, and staff scientists to present emerging work and hypotheses in the field of cancer virology. The Think Tank travel awards are provided by the Center of Excellence in HIV/AIDS & Cancer Virology, Center for Cancer Research, NCI. Members of the Freed Lab who received travel awards in previous years include Mariia Novikova and Rachel Van Duyne (2016), Philip Tedbury (2014), Angelica Martins (2013), and Emiko Urano (2013).
Research Highlights Award at 2016 NCI Scientific Retreat
In 2016, Eric Freed received a Research Highlights Award for his presentation on "Development of Potent and Broadly Active HIV-1 Maturation Inhibitors" at the NCI Annual Intramural Scientific Retreat.
Sallie Rosen Kaplan Postdoctoral Fellowship for Women Scientists
Melissa Fernandez was selected for the Sallie Rosen Kaplan (SRK) Postdoctoral Fellowship for Women Scientists at the National Cancer Institute in 2015. The SRK Fellowship is a highly competitive annual program that provides additional mentoring opportunities, networking, seminars, and workshops to help prepare NCI’s female postdoctoral fellows for the competitive nature of the job market and help them to transition to independent research careers. The highlight of this selective program is a 30-week course entitled "Career Building for Women in Science," which includes two day-long workshops. The SRK Fellowship also includes mentoring opportunities with successful women scientists from government, industry, and academia.
In 2014, Rachel Van Duyne and Mariia Novikova were selected for SRK Fellowships (2nd and 4th from left in photo below).
NIH Fellows Awards for Research Excellence
Rachel Van Duyne won a 2015 NIH Fellows Award for Research Excellence (FARE) for travel to attend and present her work at a scientific meeting in the U.S. This award, which acknowledges outstanding scientific research performed by intramural postdoctoral fellows, is sponsored by the NIH Fellows Committee, Scientific Directors, and Office of Intramural Training and Education and is funded by the Scientific Directors. FARE awards are based on scientific merit, originality, experimental design, and overall quality/presentation of the abstracts. Members of the Freed Lab who were FARE awardees in previous years include Lillian Kuo (2012), Kayoko Waki (2012), Angelica Martins (2012), Muthukumar Balasubramaniam (2010), Benjamin Luttge (2010), Catherine Adamson (2009), and Karine Gousset (2007).
2014 Outstanding Science Alumni Award, Penn State University
Eric Freed was selected by the Eberly College of Science to receive the 2014 Outstanding Science Alumni Award at Penn State University. This award recognizes alumni who have a record of significant professional achievements in their field and are outstanding role models for the current students in the college.
Award for Outstanding Scientific Presentation, 9th International Retroviral Nucleocapsid Protein and Assembly Symposium
Philip Tedbury received an award for one of the best posters at the 2013 International Retroviral Nucleocapsid Protein and Assembly Symposium in Montreal, Canada. His presentation on "Identification of a matrix mutation that globally rescues Env incorporation defects: implications for matrix structure and Env recruitment" highlighted the latest findings from his research project supported by an Intramural AIDS Research Fellowship.
Postdoctoral Fellowship, Japan Society for the Promotion of Science
Emiko Urano was awarded a Postdoctoral Fellowship from 2013 to 2015 by the Japan Society for the Promotion of Science. The fellowship program sponsored by this society supports meritorious biomedical research projects undertaken in NIH laboratories by Japanese postdoctoral researchers. Fellowships are awarded after a competitive review of research proposals.
Special Recognition as a Top Peer Reviewer for the Journal of Virology
In 2011, Eric Freed merited special recognition as one of the 25 top peer reviewers (among 1570 reviewers) for the Journal of Virology. This recognition was based on his outstanding attention to a high number of manuscripts during the year, providing insightful reviews within an exceptionally short time frame.
2010 NCI Mentor of Merit Award
Eric Freed was selected as an NCI Mentor of Merit in 2010 for excellence in mentoring and guiding the careers of trainees in cancer research. Dr. Freed was nominated by present and past trainees who view his mentorship as outstanding. NCI Director Harold Varmus presented the Mentor of Merit citation to him at the NCI Director's Awards ceremony in November 2010.
2010 Multi-Investigator Award Sponsored by Center for Cancer Research (CCR) and Office of AIDS Research (OAR)
In 2010, Eric Freed and Vineet KewalRamani of the HIV DRP, NCI-Frederick, and Sriram Subramaniam of the Laboratory of Cell Biology, NCI-Bethesda, successfully competed for a $150,000 CCR/OAR-sponsored multi-investigator award with their research proposal "Catching HIV in the act: Structural studies of HIV transport from antigen-presenting cells into the T-cell nucleus." In total, four CCR/OAR-sponsored awards were made to 11 investigators within and between the Frederick and Bethesda campuses of the National Cancer Institute.
Young Investigator Award, 2008 International Feline Retrovirus Research Symposium
Benjamin Luttge was awarded the Young Investigator Award for his oral presentation at the 2008 International Feline Retrovirus Research Symposium in Vienna, Austria.
Travel Award, 2008 NCI-Frederick Spring Research Festival Symposium
Benjamin Luttge won a travel award for the best oral presentation at the 2008 NCI-Frederick Spring Research Festival Symposium on "Virology: from Genetic Vehicles to Human Pathogens."
2006 Travel Fellowship, HIV & Cancer Virology Faculty, Center for Cancer Research
In 2006, Catherine Adamson won one of the three available travel fellowships awarded by the HIV & Cancer Virology Faculty, Center for Cancer Research, National Cancer Institute.