National Cancer Institute - Cancer.gov

LCBG Managed and Supported Resources

The Laboratory of Cancer Biology and Genetics (LCBG) manages and supports several shared resources to provide our investigators with access to cutting-edge technology and specialized expertise. These in-house resources foster collaboration and enhance the productivity, breadth, and impact of our research program.

LCBG Microscopy Facility

Manager:  Ross Lake

Patient whole-mount prostate stained for CD8 (purple), FoxP3 (red), TCF1 (orange), MHC-II (green), CD4 (yellow), and DAPI (blue).
Patient whole-mount prostate stained for CD8 (purple), FoxP3 (red), TCF1 (orange), MHC-II (green), CD4 (yellow), and DAPI (blue).

The Laboratory of Cancer Biology and Genetics (LCBG) Microscopy Facility provides training, guidance, and state-of-the-art service to LCBG scientists as well as other NCI-CCR scientists who require whole slide scanning, widefield, or confocal microscopy imaging as part of their research efforts.

Please visit the LCBG Microscopy Facility website to learn more.
 

LCBG Animal Model and Genotyping Facility

Manager:  Wendy Dubois

epresentative image demonstrating the size differences between adult mice with normal (mTOR+/+) or reduced mTOR (mTORΔ/Δ) expression, as published in Wu et al. 2013.
Representative image demonstrating the size differences between adult mice with normal (mTOR+/+) or reduced mTOR (mTORΔ/Δ) expression, as published in Wu et al. 2013.

The LCBG Animal Model and Genotyping Facility provides outstanding, reliable research support to LCBG and other NCI intramural investigators. The Facility's mission is to create and maintain mouse models for various cancers including: B cell lymphomas, plasma cell tumors, myeloma, skin carcinogenesis and melanoma, breast cancer, and prostate cancer. The Facility also provides animal study technical support (weaning, genotyping, injection administration, tumor measurement, tissue collection, and surgery) and assistance with animal study proposal submission.

Learn more about the Animal Model and Genotyping Facility.

 

LCBG High-Dimension Data Analysis Group

Manager:  Maxwell Lee, Ph.D.

Example of ChIP-seq data analysis, as published in Moshkovich et al. 2020.
Example of ChIP-seq data analysis, as published in Moshkovich et al. 2020.

The LCBG High-Dimension Data Analysis Group collaborates with LCBG investigators seeking bioinformatic support, including data analysis, development of analytic tools, result interpretation, study design, and training/education. Data types include whole genome sequencing, exome sequencing, bulk RNA-seq, single-cell RNA-seq, ChIP-seq, ATAC-seq, Methyl-seq, proteomics (RPPA), metabolomic, and microarray. Many types of analysis are supported, including:

  • Differential gene expression
  • PCA/UMAP/heatmap visualization
  • Pathway/network analysis
  • Survival analysis, clinical associations
  • Somatic and germline mutation analysis
  • DNA copy number analysis
  • Structural variation analysis
  • RNA fusion transcript analysis
  • Genome-wide association studies (GWAS) and epidemiology studies
  • Phylogenetic and population genetics
  • Constructing custom reference genomes
  • Genetic regulatory network analysis
  • Evolution dynamics
  • Epigenomics
  • Mathematics and statistical modeling

Learn more about the High-Dimension Data Analysis Group.

LCBG Molecular Pathology Unit

Manager:  R. Mark Simpson, D.V.M., Ph.D.

Representative H&E tissue staining (top) and corresponding pseudocolor markup (bottom) of identical images segmented as tumor (red) or lung plus non-neoplastic thoracic tissue (green), as published in Wei et al. 2020.
Representative H&E tissue staining (top) and corresponding pseudocolor markup (bottom) of identical images segmented as tumor (red) or lung plus non-neoplastic thoracic tissue (green), as published in Wei et al. 2020.

The LCBG Molecular Pathology Unit (MPU) provides opportunities to bridge basic and clinical research efforts by more precisely optimizing the development, characterization, and utilization of models of human disease. The MPU has extensive research and teaching expertise in comparative and investigative pathology and frequently collaborates with LCBG investigators to support:

  • Training in comparative and molecular pathology
  • Research investigation and animal model validation
  • Developing molecular diagnostics and digital pathology for research
  • Noninvasive medical imaging

Learn more about the Molecular Pathology Unit.









 

CCR Confocal Microscropy Core

Manager:  Michael J. Kruhlak, Ph.D.

Super-resolution light microscopy images of metastatic tumor cell nuclei immunolabeled against histone post-translational modifications (H3K9me3, red) and histone variants (H3.1, green).
Super-resolution light microscopy images of metastatic tumor cell nuclei immunolabeled against histone post-translational modifications (H3K9me3, red) and histone variants (H3.1, green).

The CCR Microscopy Core supports the microscopy and digital imaging needs of investigators studying the biological structures and cellular processes involved in the cell biology of cancer by applying our diverse imaging resources, expertise, and quality customer service. The goal is to provide cutting-edge imaging technology, expert consultation and training, image analysis, and data management for investigators to advance their cancer research.

Please visit the CCR Confocal Microscopy Core website to learn more.