Michael J. Kruhlak, Ph.D.

Michael J. Kruhlak, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute


Dr. Kruhlak directs the activities of the CCR Confocal Microscopy Core that supports the light microscopy imaging needs of NCI researchers. This includes developing novel imaging-based assays based on high-resolution confocal, multi-photon, and super-resolution imaging methods.

Areas of Expertise

1) confocal microscopy, 2) multi-photon imaging, 3) Airyscan super-resolution, 4) structured illumination microscopy, 5) live cell imaging, 6) lightsheet microscopy


Selected Key Publications

Generation of tumor antigen-specific iPSC-derived thymic emigrants using a 3D thymic culture system

Vizcardo R, Klemen ND, Islam SMR, Gurusamy D, Tamaoki N, Yamada D, Koseki H, Kidder BL, Yu Z, Jia L, Henning AN, Good ML, Bosch-Marce M, Maeda T, Liu C, Abdullaev Z, Pack S, Palmer DC, Stroncek DF, Ito F, Flomerfelt FA, Kruhlak MJ, and Restifo NP.
Cell Reports. 22(12): 3175-3190, 2018. [ Journal Article ]

KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis

Murgai M, Ju W, Eason M, Kline J, Beury DW, Kaczanowska S, Miettinen MM, Kruhlak M, Lei H, Shern JF, Cherepanova OA, Owens GK, Kaplan RN.
Nature Medicine. 23(10): 1176-1190, 2017. [ Journal Article ]

A macrohistone variant links dynamic chromatin compaction to BRCA1-dependent genome maintenance

Khurana S, Kruhlak MJ, Kim J, Tran AD, Liu J, Nyswaner K, Shi L, Jailwala P, Sung MH, Hakim O, and Oberdoerffer P.
Cell Reports. 8(4): 1049-1062, 2014. [ Journal Article ]

The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks

Kruhlak M, Crouch EE, Orlov M, Montano C, Gorski SA, Nussenzweig A, Misteli T, Phair RD, and Casellas R.
Nature. 447(7145): 730-734, 2007. [ Journal Article ]

Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks

Kruhlak MJ, Celeste A, Dellaire G, Fernandez-Capetillo O, Muller WG, McNally JG, Bazett-Jones DP, and Nussenzweig A.
Journal of Cell Biology. 172(6): 823-834, 2006. [ Journal Article ]


Research Biologist
Langston Lim