Michael J. Kruhlak, Ph.D.
- Center for Cancer Research
- National Cancer Institute
- Building 37, Room B114E
- Bethesda, MD 20892
- 240-858-3342
- kruhlakm@mail.nih.gov
RESEARCH SUMMARY
Dr. Kruhlak directs the activities of the CCR Confocal Microscopy Core that supports the light microscopy imaging needs of NCI researchers. This includes developing novel imaging-based assays based on high-resolution confocal, multi-photon, and super-resolution imaging methods.Areas of Expertise
1) confocal microscopy, 2) multi-photon imaging, 3) Airyscan super-resolution, 4) structured illumination microscopy, 5) live cell imaging, 6) lightsheet microscopy
Michael J. Kruhlak, Ph.D.
Publications
Selected Key Publications
Generation of tumor antigen-specific iPSC-derived thymic emigrants using a 3D thymic culture system
Cell Reports.
22(12):
3175-3190,
2018.
[ Journal Article ]
KLF4-dependent perivascular cell plasticity mediates pre-metastatic niche formation and metastasis
Nature Medicine.
23(10):
1176-1190,
2017.
[ Journal Article ]
A macrohistone variant links dynamic chromatin compaction to BRCA1-dependent genome maintenance
Cell Reports.
8(4):
1049-1062,
2014.
[ Journal Article ]
The ATM repair pathway inhibits RNA polymerase I transcription in response to chromosome breaks
Nature.
447(7145):
730-734,
2007.
[ Journal Article ]
Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks
Journal of Cell Biology.
172(6):
823-834,
2006.
[ Journal Article ]
Biography
Associate Scientist
Michael J. Kruhlak, Ph.D.
Dr. Kruhlak received his Ph.D. from the Department of Biochemistry and Molecular Biology at the University of Calgary Medical School. Â From there, in 2001, he joined the NCI as a staff scientist to head the Experimental Immunology Branch Light Microscopy and Digital Imaging Facility. In 2018, as a NCI associate scientist he became director of the CCR Confocal Microscopy Core.
