Dennis D. Hickstein, M.D.

Dennis D. Hickstein, M.D.
Senior Investigator
Head, Molecular Oncology and Gene Transfer Section

Dr. Hickstein’s laboratory is working on the recently described bone failure syndrome GATA2 deficiency. In particular, the lab is investigating mutations outside of the GATA2 exons in patients with MonoMAC syndrome, mutations associated with myeloid transformation in patients with GATA2 deficiency who have evidence of myeloid progression, and developing new approaches to the treatment of GATA2 deficiency with hematopoietic stem cell transplantation. These studies are carried out in animal models of disease and in human clinical trials.

Areas of Expertise

1) GATA2 deficiency, 2) bone marrow transplantation, 3) myelodysplastic syndrome

Contact Info

Dennis D. Hickstein, M.D.
Center for Cancer Research
National Cancer Institute
Building 10 - Hatfield CRC, Room 3-3142
Bethesda, MD 20892-1203
Ph: 240-760-6169
hicksted@mail.nih.gov

Hematopoietic Stem Cell Gene Therapy for Leukocyte Adhesion Deficiency

This project represents a pre-clinical model for gene transfer into hematopoietic stem cells. The vectors and methods identified in these studies will be used in future clinical trials of ex vivo gene therapy. For this research we are using the genetic disease, canine leukocyte adhesion deficiency (CLAD), as a model for applying advances in understanding of stem cell biology to enhance gene transfer into the hematopoietic stem cells. The ability to correct genetic hematopoietic diseases by gene therapy has a number of advantages over current therapies that involve allogeneic hematopoietic stem cell transplantation (HSCT). First, the use of autologous hematopoietic stem cells for gene therapy obviates the need for a matched bone marrow donor since each individual would serve as his or her own bone marrow stem cell donor. Second, the use of the patient's own bone marrow cells for gene replacement does not result in graft-versus-host disease (GVHD), a major complication of current allogeneic transplant. Third, use of autologous hematopoietic stem cells would be expected to enhance immune reconstitution after transplant. To date ex vivo gene therapy in hematopoietic disease has only been successful in diseases in which the transgene carries a selective advantage.

The canine form of CLAD is an optimal model for these studies because: 1) the defect involves a membrane receptor on the surface of leukocytes, flow cytometry allows facile detection and analysis of the number of gene-corrected cells; 2) low levels of gene-corrected cells result in reversal of the disease phenotype; and 3) studies in the canine model have been predictive of success in humans in the field of hematopoietic stem cell biology. The presence of a human counterpart to the canine disease allows the results from the animal model to be directly extrapolated to humans. The long-term objective is to develop strategies that will allow levels of expression of CD18 in hematopoietic cells of children with leukocyte adhesion deficiency (LAD) that are sufficient to reverse the clinical phenotype. We have used this model to test retroviral and foamy viral gene transfer into the bone marrow cells of dogs. The foamy viral vector demonstrated greater efficacy and a more favorable integration profile than the conventional retroviral vectors. The foamy viral vector-treated dogs are being followed for the durability of the correction, and for any possible genotoxicity from the vector. To date, there has been no genotoxicity. These results represent the first successful use of a foamy virus (FV) vector to treat a genetic disease, and they suggest that FV vectors will be effective in treating human hematopoietic diseases.

Hematopoietic Stem Cell Transplant for GATA2 Deficiency

The primary aim of this clinical study is to evaluate whether allogeneic HSCT reverses the hematological abnormalities in a recently described syndrome known as MonoMAC, which is characterized by: 1) onset in late adolescence or early adulthood; 2) clinical history of opportunistic infection, frequently with mycobacterium avium complex (MAC); 3) a distinct peripheral blood leukocyte subset pattern with the presence of T-lymphocytes but a severe deficiency of B-lymphocytes, natural killer (NK) cells, and the lack of monocytes; 4) dominant inheritance; and 5) frequent progression to myelodysplastic syndrome (MDS). We are evaluating whether allogeneic HSCT can reconstitute normal hematopoiesis in MonoMAC and reverse the hematological abnormalities in MDS, if MDS is present at the time of transplant. While allogeneic HSCT is the conventional curative treatment for immunodeficiency and MDS, patients with MonoMAC, because of an intact T-cell population and co-existing infection, represent a profile not generally encountered in the setting of allogeneic HSCT. The biological questions with MonoMAC center around whether the individual leukocyte compartments that are lost in MonoMAC (B-lymphocytes, NK cells, and monocytes) will be constituted with HSCT, and whether the residual recipient T-lymphocytes will represent an impediment to engraftment. We are also evaluating whether we achieve sufficient donor chimerism to reverse the MDS.

Allotransplant for DOCK8 Deficiency

Immunodeficiency associated with dedicator of cytokinesis 8 (DOCK8) deficiency represents a newly identified immunodeficiency disease characterized by a constellation of signs:  severe cutaneous and sinopulmonary infections with bacterial organisms; severe cutaneous viral infections with Herpes simplex, Herpes zoster, Molluscum contagiosum, and human papilloma virus; a marked elevation in serum IgE levels and eosinophilia; decreased numbers of CD4 and CD8 T- and B-lymphocytes; and homozygous or compound heterozygous mutations in the DOCK8 gene. Patients with this syndrome die from bacterial sepsis, squamous cell carcinomas, or hematological malignancies. HSCT represents a potentially life-saving treatment for immunodeficiency diseases due to stem cell defects. In this pilot study we are evaluating the efficacy and safety of allogeneic HSCT for DOCK8 deficiency. We are particularly interested in determining whether allogeneic HSCT reverses the lethal disease phenotype in DOCK8 deficiency by reconstituting normal host defense.

Allogeneic Hematopoietic Transplantation for Primary Immunodeficiency Diseases

With the availability of whole exome sequencing and whole genome sequencing for patients with suspected immune deficiency, the number of recognized primary immunodeficiency diseases (PIDDs) has increased in recent years to more than 400 distinct immune defects. Allogeneic HSCT represents a potentially curative therapy for many hematologic diseases and is now an accepted standard or an appropriate experimental approach for treatment of an increasing number of PIDDs. We are evaluating the efficacy and safety of allogeneic HSCT, using selected conditioning regimens and selected donor sources, in reconstituting normal hematopoiesis and immune function and reversing the disease phenotype in patients with PIDDs.

NIH Scientific Focus Areas:
Cancer Biology, Genetics and Genomics, Stem Cell Biology
View Dr. Hickstein's PubMed Summary.

Selected Key Publications

  1. Hickstein , Dennis (NIH/NCI) [E]
    J Allergy Clinical Immunology. 138(2): 628-630, 2016. [ Journal Article ]
  2. Cuellar-Rodriguez J, Freeman AF, Grossman J, Su H, Parta M, Murdock H, Shah N, Bollard C, Kong HH, Moutsopoulos N, Stone K, Gea-Banacloche J, Holland SM, Hickstein DD.
    Biol Blood Marrow Transplant. 21(6): 1037-45, 2015. [ Journal Article ]
  3. Hickstein DD, Notarangelo L.
    Blood. 130(12): 1391-93, 2017. [ Journal Article ]
  4. Shah NN, Freeman AF, Su H, Cole K, Parta M, Moutsopoulos NM, Baris S, Karakoc-Aydiner E, Hughes TE, Kong HH, Holland SM, Hickstein DD.
    Biol Blood Marrow Transplant. 23(6): 980-90, 2017. [ Journal Article ]
  5. Parta M, Shah NN, Baird K, Rafei H, Calvo KR, Hughes T, Cole K, Kenyon M, Schuver BB, Cuellar-Rodriguez J, Zerbe CS, Holland SM, Hickstein DD.
    Biol Blood Marrow Transplant. 24(6): 1250-59, 2018. [ Journal Article ]

Dr. Hickstein trained in hematology and hematopoietic stem cell transplantation at the University of Washington School of Medicine and the Fred Hutchinson Cancer Research center. He was a faculty member of the Division of hematology at the University of Washington from 1987 to 2000. He was recruited to the NCI in 2000 to transplant newly identifed primary immunodeficiency diseases.He is a member of the American Society for Clinical Investigation and the American Society of Hematology. 

Name Position
Thomas R. Bauer Jr., Ph.D. Staff Scientist
Lisa J. Embree Research Biologist
Julia Scheiermann Ph.D. Postdoctoral Fellow (Visiting)
Laura Tuschong Research Biologist
Robert West Ph.D. Research Biologist
Hatem Sabaawy Post-doctoral fellow 2002- 2007

Dr. Sabaawy is now an Associate Professor at Robert Woods Johnson Medical School in New jersey. 

Dr. Heather Shive
Heather Shive Staff Scientist 2010-2014

Dr. Shive is now an Assistant Professor in the Department of Veterinary Medicine at North Carolina State University. 

Mizuki Azuma Staff Scientist 2003-2008

Dr. Mizuki Azuma is now an Associate Professor at the University of Kansas