We offer investigators the latest instrumentation and expertise.
in the Center for Structural Biology
The CSB Cryo-EM Facility is equipped with state-of-the-art cryogenic electron microscopes and the most advanced specimen preparation equipment, including a Titan Krios G4 with Selectris/Falcon 4i, a Talos Arctica G2 with Gatan BioQuantum/K3, and an Aquilos II with iFLM and Chameleon. The Titan Krios G4 and Talos Arctica G2 can collect cryo-EM SPA data at up to 900 and 450 movies per hour, respectively. The Aquilos II is used to prepare cryo-ET lamellae samples. A full list of microscopes and specimen preparation devices can be found at Instrumentation.
Our mission is primarily to serve the CSB community, but microscope time is also available to other NCI and NIH intramural research groups; scientists whose research projects are approved can access all equipment in this facility. Non-experts can be trained in sample preparation and cryo-EM operation and collaborations can be established to allow structure determination. For detailed information and procedures on how to access this facility, see User Policies.
Instruments & Support
Three small partner proteins facilitate the type VII-dependent export of an antibacterial nuclease. mBio, accepted, 2023.Yang, Y., Boardman, E., Deme, J.C., Alcock, F., Lea, S.M. and Palmer, T.
Capturing hetergoenouse conformers of cobalamin riboswitch by cryo-EM. Nucleic Acids Res. doi: 10.1093/nar/gkad651. 2023, Ding, J., Deme, J.C., Stagno, J.R., Yu, P., Lea, S.M. and Wang, Y. X.
Structure and assembly of the NOT10:11 module of the CCR4-NOT complex. Comms Biol. 739: doi: 10.1038/s42003-023-05122-4.PMC103522412023. 2023. Levdansky, Y., Raisch, T., Deme, J., Pekovic, F., Elmlund, H., Lea, S.M. and Valkov, E.
Delineation of functional subdomains of Hungtinton protein and their interaction with HAP40. Structure, doi: 10.1016/j.str.2023.06.002. 2023. Alteen, M.G., Deme, J.C., Alvarez, C.P., Loppnau, P., Hutchinson, A., Seitova, A., Chandrasekaran, R., Ramos, E.S., Secker, C., Alqazzaz, M., Wanker, E.E., Lea, S.M., Arrowsmith, C.H. and Harding, R.J.
Structure and regulation of full-length human leucine-rich repeat kinase 1. Nature Communications volume 14(4797), 2023. R. D. Metcalfe, J. A. Martinez-Fiesco, L. Bonet-Ponce, J. H. Kluss, M. R. Cookson and P. Zhang.
MORF and MOZ acetyltransferases target unmethylated CpG islands through the winged helix domain. Nature Communications,14(697), 2023. D. C. Becht, B. J. Klein, A. Kanai, S. Jang3,10, K. L. Cox, B. Zhou, S. K. Phancr, Y. Zhang, R, Chen, C. C. Ebmeier, C. Lachance, M. Galloy, A, Fradet-Turcotte, M. L. Bulyk, Y. Bai, M. G. Poirier, J. Côté, A. Yokoyama and T. G. Kutateladze.
Structural insights into the BRAF monomer-to-dimer transition mediated by RAS binding. Nature Communications,13(486), 2022. J. A. Martinez Fiesco, D. E. Durrant, D. K. Morrison and P. Zhang.
Analysis and Comparison of Electron Radiation Damage Assessments in Cryo-EM by Single Particle Analysis and Micro-Crystal Electron Diffraction. Frontiers in Molecular Biosciences, 9, 2022. D. Shi and R. Huang.
Cryo-EM Reveals Unanchored M1-Ubiquitin Chain Bindingat hRpn11 of the 26S Proteasome. Structure, 28: 1206-1217, 2020. X. Chen, Z. Dorris, D. Shi, R. Huang, H. Khant, T. Fox, N. de Val, D. Williams, P. Zhang, and K. J. Walters.
Structure Determination from Lipidic Cubic Phase Embedded Microcrystals by MicroED. Structure 28: 1149-1159, 2020. L. Zhu, G. Bu, L. Jing, D. Shi, M. Lee, T. Gonen, W. Liu, and B. L. Nannenga.
Analysis of global and site-specific radiation damage in Cryo-EM. Structure 26(5): 759-766, 2018. J. Hattne, D. Shi, C. Glynn, C. T. Zee, M. Gallagher, M. W. Martynowycz, J. A. Rodriguez and T. Gonen.
The evolution and the advantages of MicroED. Frontiers in Molecular Biosciences, 2018. B. L. Nannenga, G. Bu and D. Shi.
MicroED structures of HIV-1 Gag CTD-SP1 reveal binding interactions with the maturation inhibitor bevirimat. Proc Natl Acad Sci USA, 115(52): 13258-13263, 2018. M. Purdy, D. Shi, J. Hattne, J. Chrustowicz, T. Gonen and M. Yeager.