The CSB Cryo-EM Facility is equipped with state-of-the-art cryogenic electron microscopes and the most advanced specimen preparation equipment, including a Titan Krios G4 with Selectris/Falcon 4i, a Talos Arctica G2 with Gatan BioQuantum/K3, and an Aquilos II with iFLM and Chameleon. The Titan Krios G4 and Talos Arctica G2 can collect cryo-EM SPA data at up to 900 and 450 movies per hour, respectively. The Aquilos II is used to prepare cryo-ET lamellae samples. A full list of microscopes and specimen preparation devices can be found at Instrumentation.

Our mission is primarily to serve the CSB community, but microscope time is also available to other NCI and NIH intramural research groups; scientists whose research projects are approved can access all equipment in this facility. Non-experts can be trained in sample preparation and cryo-EM operation and collaborations can be established to allow structure determination. For detailed information and procedures on how to access this facility, see User Policies.