Understanding the Biology of KRAS Mutant Cancer
We use genetic, molecular, pharmacological tools to study how the KRAS oncogene maintain the tumorigenic state in lung, colorectal and pancreatic cancer cells. We are particularly focused on dissecting mechanism of non-oncogene addiction in KRAS mutant cells that plays a critical role in alleviating oncogenic stress. Our work provides rationale for targeting non-oncogene addiction as a therapeutic strategy for the treatment of KRAS mutant cancer.

Non-Oncogene Addiction in KRAS Mutant Cancer
Oncogene addiction describes the cancer cell’s dependency on its driver mutation to maintain the tumorigenic state. Non-oncogene addiction describes the cancer cell’s dependency on stress-response pathways to suppress oncogenic stress and maintain viability. We were among the first to carry out synthetic lethal screens to identify non-oncogene addiction in KRAS mutant cancer cells. Our work has demonstrated that KRAS mutant cells exhibit non-oncogene addiction to multiple stress-response pathways that maintain chromosome stability, mRNA splicing, protein SUMOylation and autophagy for survival. Many of these stress-response pathways are potentially druggable.

3D In Vitro Models of KRAS Mutant Cancer
Although 2-dimensional cell culture has traditionally been a useful method to study cancer cell behavior, this model does not capture the anchorage-independent nature of cancer cell growth. We are developing scalable 3-dimensional (3D) culture models of tumor spheroid and lung organoid as an alternative and better approach to model KRAS mutant cancer in vitro. Co-culture of tumor cells with fibroblasts in these 3D models enable the characterization of tumor-stroma interaction. We are using CRISPR gene editing and chemical biology approaches in these 3D models to discover non-oncogene addiction mechanism that are critically required for the anchorage-independent growth of cancer cells.

New Therapeutic Strategies in KRAS Mutant Cells
We have developed combinatorial RNAi and CRISPR technologies to systematically interrogate the patterns of oncogene and non-oncogene addiction in KRAS mutant cells. We are exploring therapeutic potential of inhibitors targeting non-oncogene addiction pathways, oncogenic KRAS signaling in preclinical models to identify better therapeutic strategies for KRAS mutant cancer. Ultimately, we aim to translate our discoveries into better treatment outcomes for patients with KRAS mutant cancer.