Yun-Xing Wang, Ph.D.
Yun-Xing  Wang, Ph.D.
Senior Investigator
Head, Protein-Nucleic Acid Interactions Section

My group studies regulation of gene expression with emphasis on the biological events that are important for understanding causes to diseases such as cancer. We are currently studying the structural biology involving RNA structural elements in both 5' and 3' un-translated RNAs (5' and 3' UTR) as well as in coding regions of mRNAs. For example, we are studying topological folding of the HIV-1 Rev response element (RRE) RNA. The RRE RNA is a 233 nucleotide RNA and is located in the viral env coding region. We are also studying conformational flexibility of riboswitch RNAs, which regulate gene expression by alternating their conformations in response to changes in the levels of cellular metabolites.

For useful resources, go to the PNAI Download site.

Areas of Expertise
1) Nuclear magnetic resonance spectroscopy 2) RNA and protein structural biology 3) Small angle X-ray scattering

Contact Info

Yun-Xing Wang, Ph.D.
Center for Cancer Research
National Cancer Institute
Building 538, Room 116
Frederick, MD 27102-1201
301-846-5985
wangyunx@mail.nih.gov

The long-term research interests of Dr. Wang's laboratory are to understand the fundamental interactions regulating essential events involving RNA in the translational and post-translational processes on both the structural and cellular levels using NMR spectroscopy and various other biophysical, biochemical, and biological methods.  For useful resources, please go to https://ccrod.cancer.gov/confluence/display/CCRSBL1/Home

Currently, the lab focuses on the key structural element(s) in 3' and 5' prime UTR RNAs that are important for gene regulation. The lab is studying the structural basis of an adenine riboswitch, which regulates the gene expression in response to the level of adenine as a metabolite. This riboswitch is located in the 5' end of bacterial mRNA. The structural knowledge of the switching may be important to understand the fundamental mechanism of regulation of gene expression by RNA regulators.

Researchers in Dr. Wang's laboratory are also engaged in developing new methods and technologies to achieve research objectives. The Wang laboratory has been at the forefront of using small angle X-ray scattering (SAXS) to study structures and dynamics of RNAs and proteins. The laboratory has established the CCR SAXS Core Facility, which is open to all intramural and extramural research communities. For the past four years, more than 80 laboratories and research groups in the U.S. and from other countries have used the CCR SAXS resources.

Scientific Focus Areas:
Biomedical Engineering and Biophysics, Cancer Biology, Chromosome Biology, Structural Biology, Virology
Selected Recent Publications
  1. Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, García-de Los Santos A, Wang YX, Dann CE, Giedroc DP.
    J. Biol. Chem. 289: 19204-17, 2014. [ Journal Article ]
  2. Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang YX.
    Cell. 155: 594-605, 2013. [ Journal Article ]
  3. Giladi M, Sasson Y, Fang X, Hiller R, Buki T, Wang YX, Hirsch JA, Khananshvili D.
    PLoS ONE. 7: e39985, 2012. [ Journal Article ]
  4. Chen B, Zuo X, Wang YX, Dayie TK.
    Nucleic Acids Res. 40: 3117-30, 2012. [ Journal Article ]
  5. Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE.
    RNA. 18: 673-83, 2012. [ Journal Article ]

Dr. Wang conducted his graduate work on structure determination of fragments of 23S rRNA using NMR spectroscopy and UV-melting experiments in Professor David E. Draper's lab of the Johns Hopkins University. Dr. Wang received his Ph.D. in October 1994 from the Johns Hopkins University. From 1994 to 2000 he was an NIH postdoctoral later a research fellow in Dr. Dennis Torchia's laboratory where he studied the structure, hydration dynamics of HIV-1 protease in complex with inhibitors and elucidated the 3D structure and a new function of the antitumor/anti-HIV protein MAP30. In the late 2000 he then joined the Structural Biophysics Laboratory of NCI. Using high field NMR spectroscopy and other biophysical and biochemical methods, his group studies the functional structural biology of RNAs and proteins.

Name Position
Yuba Bhandari Postdoctoral Fellow (Visiting)
Yu Liu Ph.D. Postdoctoral Fellow (Visiting)
Jason Stagno Ph.D. Staff Scientist
Monalisa Swain Ph.D. Postdoctoral Fellow (CRTA)
Derek Wendel Student Intern
Ping Yu Laboratory Technician (Contr)

Research

The long-term research interests of Dr. Wang's laboratory are to understand the fundamental interactions regulating essential events involving RNA in the translational and post-translational processes on both the structural and cellular levels using NMR spectroscopy and various other biophysical, biochemical, and biological methods.  For useful resources, please go to https://ccrod.cancer.gov/confluence/display/CCRSBL1/Home

Currently, the lab focuses on the key structural element(s) in 3' and 5' prime UTR RNAs that are important for gene regulation. The lab is studying the structural basis of an adenine riboswitch, which regulates the gene expression in response to the level of adenine as a metabolite. This riboswitch is located in the 5' end of bacterial mRNA. The structural knowledge of the switching may be important to understand the fundamental mechanism of regulation of gene expression by RNA regulators.

Researchers in Dr. Wang's laboratory are also engaged in developing new methods and technologies to achieve research objectives. The Wang laboratory has been at the forefront of using small angle X-ray scattering (SAXS) to study structures and dynamics of RNAs and proteins. The laboratory has established the CCR SAXS Core Facility, which is open to all intramural and extramural research communities. For the past four years, more than 80 laboratories and research groups in the U.S. and from other countries have used the CCR SAXS resources.

Scientific Focus Areas:
Biomedical Engineering and Biophysics, Cancer Biology, Chromosome Biology, Structural Biology, Virology

Publications

Selected Recent Publications
  1. Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, García-de Los Santos A, Wang YX, Dann CE, Giedroc DP.
    J. Biol. Chem. 289: 19204-17, 2014. [ Journal Article ]
  2. Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang YX.
    Cell. 155: 594-605, 2013. [ Journal Article ]
  3. Giladi M, Sasson Y, Fang X, Hiller R, Buki T, Wang YX, Hirsch JA, Khananshvili D.
    PLoS ONE. 7: e39985, 2012. [ Journal Article ]
  4. Chen B, Zuo X, Wang YX, Dayie TK.
    Nucleic Acids Res. 40: 3117-30, 2012. [ Journal Article ]
  5. Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE.
    RNA. 18: 673-83, 2012. [ Journal Article ]

Biography

Dr. Wang conducted his graduate work on structure determination of fragments of 23S rRNA using NMR spectroscopy and UV-melting experiments in Professor David E. Draper's lab of the Johns Hopkins University. Dr. Wang received his Ph.D. in October 1994 from the Johns Hopkins University. From 1994 to 2000 he was an NIH postdoctoral later a research fellow in Dr. Dennis Torchia's laboratory where he studied the structure, hydration dynamics of HIV-1 protease in complex with inhibitors and elucidated the 3D structure and a new function of the antitumor/anti-HIV protein MAP30. In the late 2000 he then joined the Structural Biophysics Laboratory of NCI. Using high field NMR spectroscopy and other biophysical and biochemical methods, his group studies the functional structural biology of RNAs and proteins.

Team

Name Position
Yuba Bhandari Postdoctoral Fellow (Visiting)
Yu Liu Ph.D. Postdoctoral Fellow (Visiting)
Jason Stagno Ph.D. Staff Scientist
Monalisa Swain Ph.D. Postdoctoral Fellow (CRTA)
Derek Wendel Student Intern
Ping Yu Laboratory Technician (Contr)