Ping Zhang, Ph.D.
My laboratory studies the structural and mechanistic basis of multi-component kinase signaling complexes that are linked to human cancers and other diseases, with a long-term goal of developing new therapeutic strategies. Current research topics include an oncogenic PKA kinase fusion protein, the Raf family kinases and the leucine rich repeat kinases. We apply integrated structural biology (single-particle cryo-electron microscopy and X-ray crystallography) and biochemical approaches to achieve our objective of studying these kinase complexes in their functional states. This strategy is used to reveal the mechanistic details and factors critical for driving the functional activities of these kinases and how these activities may be altered in pathological states.
1) kinase, 2) cell signaling, 3) cancer biology, 4) structural biology
We aim to combine structural and functional studies to reveal the molecular mechanism of kinase complexes in health and disease and help to facilitate the development of new strategies for therapeutic intervention.
Selected Recent Publications
Two PKA RIα Holoenzyme States Define ATP as an Isoform-Specific Orthosteric Inhibitor That Competes With the Allosteric Activator, cAMP.Proc Natl Acad Sci U S A. 116(33): 16347-56, 2019. [ Journal Article ]
Atomic Resolution cryo-EM Structure of a Native-Like CENP-A Nucleosome Aided by an Antibody Fragment.Nat Commun. 10(1): 2301, 2019. [ Journal Article ]
- Structure. 27(5): 816-28, 2019. [ Journal Article ]
- J Mol Biol. 430(18 Pt B): 3093-3110, 2018. [ Journal Article ]
- PLoS Biol. 13(7): e1002192, 2015. [ Journal Article ]
Dr. Zhang received her Ph.D. training in Dr. Michael Rossmann’s lab at Purdue University in the field of biochemistry and structural virology. Her Ph.D. project was resolving the structures of poliovirus-receptor complexes using X-ray crystallography and cryo-electron microscopy (cryo-EM). She completed her postdoctoral training in Dr. Susan Taylor’s laboratory at UCSD, working on a signal transduction system related to human diseases and learning other techniques in structural biology and cell signaling that are suited for studying dynamic signaling complexes. She joined the Structural Biophysics Laboratory at NCI as a Stadtman Tenure Track Investigator in August 2016.
|Ning Li Ph.D.||Research Fellow|
|Juliana Andrea Martinez Fiesco Ph.D.||Postdoctoral Fellow (Visiting)|
Current position: Scientist, University of Bristol
Current position: Principle investigator, Beijing Tuojie Biomedical Technology Co., Ltd
Current position: Staff Scientist, Thermo Fisher Scientific, Boston