Yun-Xing Wang, Ph.D.

Yun-Xing  Wang, Ph.D.
Senior Investigator
Head, Protein-Nucleic Acid Interactions Section
Head, CCR Small Angle X-ray Scattering Core Facility

Yun-Xing Wang is a structural biologist who pioneered the combined use of NMR spectroscopy, small angle X-ray scattering (SAXS), diffraction and other biophysical methods to study RNA structural biology.  His laboratory research places emphases on solving long-standing important structural biology questions and uses the gained knowledge to develop diagnostic and therapeutic agents for cancer and HIV/AIDS.  His laboratory also invented the first RNA labeling “machine”.

Visit the SAXS Core Facility for more information about this new resource.

For useful resources, visit the Protein-Nucleic Acid Interactions wiki site.

Areas of Expertise
1) RNA Structural Biology 2) Protein Structural Biology 3) NMR 4) SAXS 5) X-ray Free Electron Laser and Serial Crystallography

Contact Info

Yun-Xing Wang, Ph.D.
Center for Cancer Research
National Cancer Institute
Advanced Technology Research Facility (ATRF)/B3340
Frederick, MD 27102-1201
Ph: 301-846-5985
wangyunx@mail.nih.gov

My laboratory studies the RNA structural biology.  We use biophysical methods, including NMR spectroscopy, SAXS, and X-ray diffraction, to determine RNA three-dimensional structures or topological folds and to understand RNA function.  Our studies provide insight at the atomic level into fundamental mechanisms of RNA biological functions.  This knowledge is the key to the development of novel diagnostic and therapeutic reagents for cancer and AIDS.

RNA function is coded in its three-dimensional structures and dynamics.  To characterize RNA in four-dimensional space, we have resorted to new technologies that allow us to study ever more challenging problems.  For this reason, our laboratory has developed Position-selective Labeling of RNA (PLOR) technology, which can be applied for structural biology studies, imaging and disease detection. Currently, we are developing a new approach using X-ray free electron laser (XFEL) to study RNA structures and dynamics.

Scientific Focus Areas:
Biomedical Engineering and Biophysics, Cancer Biology, Chromosome Biology, Structural Biology, Virology
View Dr. Wang's PubMed Summary.

Selected Recent Publications

  1. Stagno JR, Liu Y, Bhandari YR, Conrad CE, Panja S, Swain M, Fan L, Nelson G, Li C, Wendel DR, White TA, Coe JD, Wiedorn MO, Knoska J, Oberthuer D, Tuckey RA, Yu P, Dyba M, Tarasov SG, Weierstall U, Grant TD, Schwieters CD, Zhang J, Ferré-D'Amaré AR, Fromme P, Draper DE, Liang M, Hunter MS, Boutet S, Tan K, Zuo X, Ji X, Barty A, Zatsepin NA, Chapman HN, Spence JC, Woodson SA, Wang YX.
    Nature. 541/7636: 242-246, 2017. [ Journal Article ]
  2. Liu Y, Holmstrom E, Zhang J, Yu P, Wang J, Dyba MA, De Chen, Ying J, Lockett S, Nesbitt DJ, Ferré-D'Amaré AR, Sousa R, Stagno JR, and Wang Y-X.
    Nature. 522: 368-372, 2015. [ Journal Article ]
  3. Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang YX.
    Cell. 155: 594-605, 2013. [ Journal Article ]
  4. Zuo X, Wang J, Yu P, Eyler D, Xu H, Starich MR, Tiede DM, Simon AE, Kasprzak W, Schwieters CD, Shapiro, BA, and Wang Y-X.
    Proc. Natl. Acad. Sci. U. S. A. 107: 1385-1390, 2010. [ Journal Article ]
  5. Zuo X, Wang J, Foster TR, Schwieters CD, Tiede DM, Butcher SE, and Wang Y-X.
    J. Am. Chem. Soc. 130: 3292-3293, 2008. [ Journal Article ]

Dr. Wang conducted his graduate work on structure determination of fragments of 23S rRNA using NMR spectroscopy and UV-melting experiments in Professor David E. Draper's lab of the Johns Hopkins University. Dr. Wang received his Ph.D. in October 1994 from the Johns Hopkins University. From 1994 to 2000 he was an NIH postdoctoral later a research fellow in Dr. Dennis Torchia's laboratory where he studied the structure, hydration dynamics of HIV-1 protease in complex with inhibitors and elucidated the 3D structure and a new function of the antitumor/anti-HIV protein MAP30. In the late 2000 he then joined the Structural Biophysics Laboratory of NCI. Using high field NMR spectroscopy and other biophysical and biochemical methods, his group studies the functional structural biology of RNAs and proteins.

Name Position
Jason R. Stagno, Ph.D. Associate Scientist
Ping Yu Biologist
Yuba Bhandari Ph.D. Postdoctoral Fellow (CRTA)
Negin Orfanian Azimzadeh Khosravi Postbaccalaureate Fellow (CRTA)
Chelsie E. Smith Ph.D. Postdoctoral Fellow (CRTA)