Avinash Bhandoola, M.B., B.S., Ph.D.

Profile photo of Avinash Bhandoola, Ph.D.
Senior Investigator
Head, T-Cell Biology and Development Unit

T cells play essential roles in immune responses. They originate from blood stem cells in the bone marrow, but their development completes at a distinct anatomical site, the thymus. We're interested in the mechanisms by which some hematopoietic progenitor cells migrate to the thymus, and in the transcriptional mechanisms in the thymus that direct the earliest thymus colonizing progenitors down the T cell lineage. We hope to understand the regulatory and developmental logic that establishes a functioning immune system.

Areas of Expertise
1) transcription factors that establish and maintain T cell identity, 2) T cell regeneration and bone marrow transplantation, 3) thymic epithelial cells

Contact Info

Avinash Bhandoola, M.B., B.S., Ph.D.
Center for Cancer Research
National Cancer Institute
Building 37, Room 1108
Bethesda, MD 20892-4254
Ph: 240-760-7592
Fax: 240-541-4489
avinash.bhandoola@nih.gov

T cell Development and Regeneration

T cells play essential roles in immune responses. We study how these functional abilities of T cells are acquired during their development. Like other blood cells, T cells originate from blood stem cells resident in the bone marrow. Uniquely among blood cells, their development completes at a distinct anatomical site, the thymus. We're interested in the mechanisms by which some hematopoietic progenitor cells migrate to the thymus. We're further interested in the transcriptional mechanisms in the thymus that direct the earliest thymus colonizing progenitors down the T cell lineage. We hope to understand the regulatory and developmental logic that establishes a functioning immune system.

LGI Branch 2014

The Laboratory of Genome Integrity: December 2014
Seated (L to R): Amanda Day, Subhadra Banerjee, Ludmila Krymskaya, Christelle Harly, David McCurdy, Avinash Bhandoola (Senior Investigator), Andre Nussenzweig (Laboratory Chief), Henry Chen, Andres Canela Rodriguez and Nancy Wong
Standing (L to R): Karen Wolcott, Sam John, Anthony Tubbs, Caiyi Li, Pulak Ranjan Nath, Dali Zong, Arnab Ray Chaudhuri, Margarida Santos, Khadidiatou Waye, Elsa Callen Moreu (Staff Scientist), Andre Stanlie, Aysegul Ergen and Robert Faryabi

Current projects in the laboratory include:

1) Migration of hematopoietic progenitors to the thymus. We are studying the signals that permit circulating hematopoietic progenitors to selectively settle within the thymus from the blood, and how these signals change during thymic regeneration. We're interested in whether improved knowledge of thymic homing can be used to enhance migration to the thymus after bone marrow transplantation. (Schwarz and Bhandoola, Nat. Immunol., 2004;  Zlotoff,  et al., Blood, 2010;  Zlotoff,  Zhang, et al., Blood, 2011;  Sultana, et al., J. Immunol., 2012;  Zhang, et al., Blood  2014)

2) Transcription factors that establish and maintain T-cell specific gene expression. TCF-1 and HES-1 are transcription factors that impose T cell identity early in T cell development, but the mechanisms employed are uncertain, and are the focus of intense investigation in our laboratory. We currently think HES-1 represses genes involved in development of non-T cell lineages, whereas TCF-1 may drive T-lineage specific gene expression. We're using conditional alleles of TCF-1 to determine whether TCF-1 controls expression of a common set of T cell genes throughout development, or if TCF-1 instead plays different roles in mature T cells. (Sambandam, et al., Nat. Immunol., 2005;  Weber, Chi, et al., Nature, 2011;  De Obaldia, et al., Blood, 2013;  De Obaldia, et al., Nat. Immunol., 2013)

3) The development and function of innate lymphoid cells. Innate lymphoid cells have transcriptional programs that appear to mirror those of T cells. The comparison of innate lymphocyte cell development with T cell development provides an opportunity to understand the factors that underlie the shared as well as the unique features and functions of these apparently closely related cell lineages. (Yang, et al., J. Immunol., 2011;  Yang, et al., Immunity, 2013)

4) Transcription factors underlying the development, maintenance and regeneration of thymic epithelial cells.

Scientific Focus Areas:
Cancer Biology, Cell Biology, Developmental Biology, Immunology, Stem Cell Biology
View Dr. Bhandoola's PubMed Summary.

Selected Key Publications

  1. Zhang SL, Wang X, Manna S, Zlotoff DA, Bryson JL, Blazar BR, Bhandoola A.
    Blood. 124: 296-304, 2014. [ Journal Article ]
  2. De Obaldia ME, Bell JJ, Wang X, Harly C, Yashiro-Ohtani Y, DeLong JH, Zlotoff DA, Sultana DA, Pear WS, Bhandoola A.
    Nat. Immunol. 14: 1277-84, 2013. [ Journal Article ]
  3. Yang Q, Monticelli LA, Saenz SA, Chi AW, Sonnenberg GF, Tang J, De Obaldia ME, Bailis W, Bryson JL, Toscano K, Huang J, Haczku A, Pear WS, Artis D, Bhandoola A.
    Immunity. 38: 1277-84, 2013. [ Journal Article ]
  4. Weber BN, Chi AW, Chavez A, Yashiro-Ohtani Y, Yang Q, Shestova O, Bhandoola A.
    Nature. 476: 63-8, 2011. [ Journal Article ]
  5. Bell JJ, Bhandoola A.
    Nature. 452: 764-7, 2008. [ Journal Article ]

Avinash Bhandoola received a Ph.D. from the University of Pennsylvania in 1994, and did a postdoctoral fellowship with Dr. Alfred Singer at NCI focused on T cell development. He joined the faculty of the University of Pennsylvania in 2001, received tenure in 2007, and was promoted to full professor in 2012. He subsequently joined the Laboratory of Genome Integrity in 2014.

Name Position
Jennifer E. Cowan Ph.D. Postdoctoral Fellow (Visiting)
Arundhoti Das Ph.D. Postdoctoral Fellow (Visiting)
Christelle Harly Ph.D. Research Fellow
Devin Kenney Postbaccalaureate Fellow (CRTA)
Brian Larsen Ph.D. Postdoctoral Fellow (Visiting)
Brittany Townley Postbaccalaureate Fellow (CRTA)
Qi Yang Guest Researcher
Yongge Zhao Ph.D. Biologist