Nathanael D. Pruett, Ph.D.

Nathanael D. Pruett, Ph.D.
Research Biologist

Team Member of:

Dr. Pruett’s research focuses on elucidating the oncogenic regulatory mechanisms underlying the establishment and progression of mesothelioma focusing on the role inflammation as a causative signaling pathway. To do this, he is developing oncogenic models with which to evaluate contributing signal transduction pathways and resulting gene expression patterns with the ultimate goal of identifying potential clinically translatable targets.

Areas of Expertise

1) CRISPR-mediated genome editing
2) transcriptome profiling—NGS-based
3) epigenetic gene regulation
4) cancer cell biology
5) mesothelioma

Contact Info

Nathanael D. Pruett, Ph.D.
Center for Cancer Research
National Cancer Institute
Building 10-CRC, Room 3-5888
Bethesda, MD 20892-1201
Ph: 240-858-3736
nathanael.pruett@nih.gov

Dr. Pruett’s research focuses on elucidating the oncogenic regulatory mechanisms underlying the establishment and progression of mesothelioma focusing on the role inflammation as a causative signaling pathway. To do this, he is developing oncogenic models with which to evaluate contributing signal transduction pathways and resulting gene expression patterns with the ultimate goal of identifying potential clinically translatable targets.

NIH Scientific Focus Areas:
Cancer Biology, Cell Biology, Genetics and Genomics, Molecular Biology and Biochemistry

Selected Publications

  1. Vian L, Pękowska A, Rao SSP, Kieffer-Kwon KR, Jung S, Baranello L, Huang SC, El Khattabi L, Dose M, Pruett N, Sanborn AL, Canela A, Maman Y, Oksanen A, Resch W, Li X, Lee B, Kovalchuk AL, Tang Z, Nelson S, Di Pierro M, Cheng RR, Machol I, St Hilaire BG, Durand NC, Shamim MS, Stamenova EK, Onuchic JN, Ruan Y, Nussenzweig A, Levens D, Aiden EL, Casellas R.
    Cell. 173(5): 1165-1178, 2018. [ Journal Article ]
  2. Singh A, Pruett N, Hoang CD.
    Transl Lung Cancer Res. 6(3): 248-258, 2017. [ Journal Article ]
  3. Qian J, Wang Q, Dose M, Pruett N, Kieffer-Kwon KR, Resch W, Liang G, Tang Z, Mathé E, Benner C, Dubois W, Nelson S, Vian L, Oliveira TY, Jankovic M, Hakim O, Gazumyan A, Pavri R, Awasthi P, Song B, Liu G, Chen L, Zhu S, Feigenbaum L, Staudt L, Murre C, Ruan Y, Robbiani DF, Pan-Hammarström Q, Nussenzweig MC, Casellas R.
    Cell. 159(7): 1524-37, 2014. [ Journal Article ]
  4. Kieffer-Kwon KR, Tang Z, Mathe E, Qian J, Sung MH, Li G, Resch W, Baek S, Pruett N, Grøntved L, Vian L, Nelson S, Zare H, Hakim O, Reyon D, Yamane A, Nakahashi H, Kovalchuk AL, Zou J, Joung JK, Sartorelli V, Wei CL, Ruan X, Hager GL, Ruan Y, Casellas R.
    Cell. 155(7): 1507-20, 2013. [ Journal Article ]
  5. Nakahashi H, Kieffer Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R.
    Cell Rep. 3(5): 1678-1689, 2013. [ Journal Article ]

Dr. Pruett received his Ph.D. degree in Biomedical Sciences from the Medical University of South Carolina in 2011 where his studies focused on cardiovascular biology and developmental biology. He joined the National Institutes of Health as a postdoctoral trainee in the Laboratory of Molecular Immunogenetics headed by Dr. Raphael Casellas, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Genomics and Immunity Section. Here Dr. Pruett worked to advance the goal of the lab to better understand the genetic and molecular pathways that drive the immune response under normal and pathological conditions. His contributions were recognized in 2014 by being presented with the NIH Director’s Award for his involvement in establishing and executing the NIH Mouse Regulome Project. In January 2017 he joined Dr. Hoang’s lab as a Research Biologist.