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Shuo  Gu, Ph.D.

Shuo Gu, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
  • Building 560, Room 11-83B
  • Frederick, MD 21702
  • 301-846-5447
  • shuo.gu@nih.gov
RNA Biology Laboratory

RESEARCH SUMMARY

Dr. Gu’s research focuses on the mechanisms of RNA interference and microRNA pathways, and their applications in cancer treatment. MiRNAs play essential roles in gene regulation networks, human diseases and cancer. Research in the Gu lab aims to unveil the mechanisms of miRNA biogenesis, post-transcriptional modifications, and biological functions in mammalian systems. This information will then be used to test novel RNA-based approaches designed to alter gene expression with improved safety, off-targeting, and potency profiles, which can then be used as tools for biological discovery and therapeutics.

Areas of Expertise

1) RNAi, 2) miRNA, 3) non-coding RNA, 4) gene therapy, 5) RNA biology

Publications

Selected Recent Publications

TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

Yang A, Bofill-De Ros X, Stanton R, Shao TJ, Villanueva P, Gu S.
Nature Communications. 13(1): 5260, 2022.
Full-Text Article
[ Journal Article ]

AGO-bound Mature miRNAs Are Oligouridylated by TUTs and Subsequently Degraded by DIS3L2

Yang A, Shao TJ, Bofill-De Ros X, Lian C, Villanueva P, Dai L, Gu S.
Nat Commun. 2020. [ Journal Article ]

3' Uridylation Confers miRNAs with Non-canonical Target Repertories

Yang A, Bofill-De Ros X, Shao TJ, Jiang M, Li K, Villanueva P, Dai L, Gu S.
Mol Cell. May 25: 2019. [ Journal Article ]

Structural Differences between Pri-miRNA Paralogs Promote Alternative Drosha Cleavage and Expand Target Repertoires

Bofill-De Ros X, Kasprzak WK, Bhandari Y, Fan L, Cavanaugh Q, Jiang M, Dai L, Yang A, Shao TJ, Shapiro BA, Wang YX, Gu S.
Cell reports. 26: 447-459, 2019. [ Journal Article ]

Cytoplasmic Drosha activity generated by alternative splicing.

Dai L, Chen K, Youngren B, Kulina J, Yang A, Guo Z, Li J, Yu P, Gu S.
Nucleic Acids Res. 2016 Dec 1;44(21): 10454-10466, 2016. [ Journal Article ]

Job Vacancies

There are no open positions at this time. Check back again later, or take a look at CCR's Careers page.

Team

Postdoctoral Fellow (Visiting)
Xavier Bofill de Ros, Ph.D.
Postdoctoral Fellow (Visiting)
Sharan Malagobadan, Ph.D.
Postbaccalaureate Fellow (CRTA)
Alexi Misciagna
Predoctoral Fellow (Graduate Student)
James G. Shamul
Postdoctoral Fellow (CRTA)
Chunmei Shi, M.D., Ph.D.
Postbaccalaureate Fellow (CRTA)
Karrie Spain

Covers

Cell Reports Cover January 2019

Structural Differences between Pri-miRNA Paralogs Promote Alternative Drosha Cleavage and Expand Target Repertoires

Published Date

MicroRNA biogenesis begins with Drosha cleavage, the fidelity of which is critical for downstream processing and mature miRNA target specificity. Bofill-De Ros et al. studied the maturation of three pri-miRNAs, which encode the same mature miRNA but differ in the surrounding structure. They see altered cleavage due to distorted and flexible stem structure of one of the miRNAs and show that distortion in the stem appears to be a general mechanism for 5′ isoform generation. Image of pri-miRNA structures and Drosha created by Xavier Bofill-De Ros

Citation

Xavier Bofill-De Ros et.al., Cell Reports, Volume 26, ISSUE 2, P447-459.e4, January 08, 2019