Nathanael D. Pruett, Ph.D.

Nathanael D. Pruett, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
Thoracic Surgery Branch


Dr. Pruett’s research focuses on elucidating the oncogenic regulatory mechanisms underlying the establishment and progression of mesothelioma focusing on the role inflammation as a causative signaling pathway. To do this, he is developing oncogenic models with which to evaluate contributing signal transduction pathways and resulting gene expression patterns with the ultimate goal of identifying potential clinically translatable targets.

Areas of Expertise

1) CRISPR-mediated genome editing 2) transcriptome profiling—NGS-based 3) epigenetic gene regulation 4) cancer cell biology 5) mesothelioma


Selected Publications

The Energetics and Physiological Impact of Cohesin Extrusion

Vian L, Pękowska A, Rao SSP, Kieffer-Kwon KR, Jung S, Baranello L, Huang SC, El Khattabi L, Dose M, Pruett N, Sanborn AL, Canela A, Maman Y, Oksanen A, Resch W, Li X, Lee B, Kovalchuk AL, Tang Z, Nelson S, Di Pierro M, Cheng RR, Machol I, St Hilaire BG, Durand NC, Shamim MS, Stamenova EK, Onuchic JN, Ruan Y, Nussenzweig A, Levens D, Aiden EL, Casellas R.
Cell. 173(5): 1165-1178, 2018. [ Journal Article ]

In vitro experimental models of mesothelioma revisited

Singh A, Pruett N, Hoang CD.
Transl Lung Cancer Res. 6(3): 248-258, 2017. [ Journal Article ]

B cell super-enhancers and regulatory clusters recruit AID tumorigenic activity

Qian J, Wang Q, Dose M, Pruett N, Kieffer-Kwon KR, Resch W, Liang G, Tang Z, Mathé E, Benner C, Dubois W, Nelson S, Vian L, Oliveira TY, Jankovic M, Hakim O, Gazumyan A, Pavri R, Awasthi P, Song B, Liu G, Chen L, Zhu S, Feigenbaum L, Staudt L, Murre C, Ruan Y, Robbiani DF, Pan-Hammarström Q, Nussenzweig MC, Casellas R.
Cell. 159(7): 1524-37, 2014. [ Journal Article ]

Interactome maps of mouse gene regulatory domains reveal basic principles of transcriptional regulation

Kieffer-Kwon KR, Tang Z, Mathe E, Qian J, Sung MH, Li G, Resch W, Baek S, Pruett N, Grøntved L, Vian L, Nelson S, Zare H, Hakim O, Reyon D, Yamane A, Nakahashi H, Kovalchuk AL, Zou J, Joung JK, Sartorelli V, Wei CL, Ruan X, Hager GL, Ruan Y, Casellas R.
Cell. 155(7): 1507-20, 2013. [ Journal Article ]

A genome-wide map of CTCF multivalency redefines the CTCF code

Nakahashi H, Kieffer Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, Pugh BF, Lobanenkov V, Hager GL, Casellas R.
Cell Rep. 3(5): 1678-1689, 2013. [ Journal Article ]