Photo of Jason Rausch, Ph.D.

Jason W. Rausch, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute


The primary focus of my research has been to use various biochemistry, molecular biology and computer modeling tools to study the structures and functions of HIV proteins (reverse transcriptase, integrase, capsid, nucleocapsid, rev), HIV-related host proteins (APOBEC3G, DC-SIGN, L-SIGN) and viral RNA motifs (HIV-1 polypurine tracts, HIV-1 and HIV-2 rev-response elements, HIV-1 5’ UTR). My recent emphasis has been on determining the secondary structures of HIV-related RNAs using the chemical probing methodology known as selective 2’ hydroxyl acylation analyzed by primer extension (SHAPE) and expanding this technology for use with next generation sequencing systems.

Areas of Expertise

HIV (human Immunodeficiency Virus)
In Vitro Assay Development
RNA Structure
Next-generation Sequencing
Molecular Modeling


Selected Publications

Novel biochemical tools for probing HIV RNA structure

Rausch JW, Sztuba-Solinska J, Lusvarghi S, Le Grice SF.
Methods Mol. Biol. 1354: 91-117, 2016. [ Journal Article ]

SiRNA-induced mutation in HIV-1 polypurine tract region and its influence on viral fitness

Rausch JW, Tian M, Li Y, Angelova L, Bagaya BS, Krebs KC, Qian F, Zhu C, Arts EJ, Le Grice SF, Gao Y.
PLOS One. 10: e0122953, 2015. [ Journal Article ]

An unusual topological structure of the HIV-1 Rev response element

Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang Y.
Cell. 155: 594-605, 2013. [ Journal Article ]

The HIV-2 Rev-response element: determining secondary structure and defining folding intermediates

Lusvarghi S, Sztuba-Solinska J, Purzycka KJ, Pauly GT, Rausch JW, Grice SF.
Nucleic Acids Res. 41: 6637-49, 2013. [ Journal Article ]

Progress towards developing potent and specific inhibitors of HIV RT-associated ribonuclease H

Rausch J. Le Grice S, Goette M, eds.
143-172, 2013. [ Book Chapter ]