Gianluca  Pegoraro, Ph.D.

Gianluca Pegoraro, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute


Dr. Pegoraro heads the High-Throughout Imaging Facility (HiTIF), which provides CCR investigators with the expertise and the technology needed to set-up, optimize and implement high-content imaging (HCI) assays. HCI assays are used to analyze large numbers of experimental conditions, such as in functional genomics or chemical compounds screens. In addition, the HiTIF applies HCI in combination with single-cell image and data analysis to quantify exceedingly rare but biologically important events in heterogeneous populations of cells.

Areas of Expertise

High-content Imaging
Functional Genomics


Selected Key Publications

Identification of epigenetic modulators as determinants of nuclear size and shape

Schibler, AC; Jevtic, P; Pegoraro, G; Levy, Daniel L; Misteli, T
Elife. 12: 2023.
Full-Text Article
[ Journal Article ]

HiFENS: high-throughput FISH detection of endogenous pre-mRNA splicing isoforms

Shilo, A; Pegoraro, G; Misteli, T
Nucleic Acids Res.. 2022.
Full-Text Article
[ Journal Article ]

High-throughput imaging of mRNA at the single-cell level in human primary immune cells

Gadkari, M; Sun, J; Carcamo, Adrian; Alessi, H; Hu, Zi; Fraser, IDC; Pegoraro, G; Franco, LM
RNA. 28/9: 1263-1278, 2022.
Full-Text Article
[ Journal Article ]

Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and stochastic splice site selection

Wan, Yihan; Anastasakis, Dimitrios G; Rodriguez, Joseph; Palangat, Murali; Gudla, Prabhakar; Zaki, George; Tandon, Mayank; Pegoraro, Gianluca; Chow, Carson C; Hafner, Markus; Larson, Daniel R
Cell. 184(11): 2878-2895.e20, 2021.
Full-Text Article
[ Journal Article ]

A Deep Learning Pipeline for Nucleus Segmentation

Zaki, George; Gudla, Prabhakar R; Lee, Kyunghun; Kim, Justin; Ozbun, Laurent; Shachar, Sigal; Gadkari, Manasi; Sun, Jing; Fraser, Iain D C; Franco, Luis M; Misteli, Tom; Pegoraro, Gianluca
Cytometry Part A. 97(12): 1248-1264, 2020.
Full-Text Article
[ Journal Article ]


Staff Scientist
Adib Keikhosravi , Ph.D.
Picture of Dr. Sagar
Staff Scientist
Md Abdul Kader Sagar, Ph.D.