Don Farthing, Ph.D.

Don Farthing, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
Center for Immuno-Oncology


Don Farthing heads the mass spectrometry and epitope discovery facility (MSEDF) that supports preclinical and clinical researchers from the Center for Immuno-Oncology. The facility has tandem mass spectrometers (UPLC-PDA-QTof, HS-GC-QqQ) that are utilized to support translational research (e.g., development of adoptive T cell therapies, graft- versus-host disease, deuterium imaging of rapidly proliferating cells). 

 US Patent and Trademark Office patent, US 11,684,306. Buxbaum, N.P. (lead inventor) et al, “Stable water isotope labeling and magnetic resonance imaging for visualization of rapidly dividing cells”, issued June 27, 2023.…

Areas of Expertise

Chromatography-mass Spectrometry
Analytical Chemistry
Pharmacokinetic/pharmacodynamic (PK/PD) Analysis
Clinical Pharmacology


Selected Publications

Glycolytic metabolism of pathogenic T cells enables early detection of GVHD by 13C-MRI

Assmann, JC, Farthing, DE, Saito, K, Maglakelidze, N, Oliver, B, Warrick, KA, Sourbier, C, Ricketts, CJ, Meyer, TJ, Pavletic, SZ, Linehan, WM, Krishna, MC, Gress, RE and Buxbaum, NP
Blood. 137/1: 126-137, 2021.
Full-Text Article
[ Journal Article ]

Comparing DNA enrichment of proliferating cells following administration of different stable isotopes of heavy water

Farthing, DE, Buxbaum, NP, Lucas, PJ, Maglakelidze, N, Oliver, B, Wang, J, Hu, K, Castro, E, Bare, CV and Gress, RE
Sci Rep. 7/1: 4043, 2017.
Full-Text Article
[ Journal Article ]

In vivo kinetics and nonradioactive imaging of rapidly proliferating cells in graft-versus-host disease

Buxbaum, NP, Farthing, DE, Maglakelidze, N, Lizak, M, Merkle, H, Carpenter, AC, Oliver, BU, Kapoor, V, Castro, E, Swan, GA, Dos Santos, LM, Bouladoux, NJ, Bare, CV, Flomerfelt, FA, Eckhaus, MA, Telford, WG, Belkaid, Y, Bosselut, RJ and Gress, RE
JCI Insight. 2/12: 2017.
Full-Text Article

Prospective Phase II Trial of Montelukast to Treat Bronchiolitis Obliterans Syndrome after Hematopoietic Cell Transplantation and Investigation into Bronchiolitis Obliterans Syndrome Pathogenesis

Williams KM, Pavletic SZ, Lee SJ, Martin PJ, Farthing DE, Hakim FT, Rose J, Manning-Geist BL, Gea-Banacloche JC, Comis LE, Cowen EW, Justus DG, Baird K, Cheng GS, Avila D, Steinberg SM, Mitchell SA, Gress RE
Transplant Cell Ther.. May;28(5): 264.e1-264.e9, 2022. [ Journal Article ]

In vivo deuterium magnetic resonance imaging of xenografted tumors following systemic administration of deuterated water

Brender JR, Assmann JC, Farthing DE, Saito K, Kishimoto S, Warrick KA, Maglakelidze N, Larus TL, Merkle H, Gress RE, Krishna MC, Buxbaum NP
Sci Rep. Sep 7;13(1): 14699, 2023. [ Journal Article ]