Xiang Chen, Ph.D.

Xiang Chen, Ph.D.
Staff Scientist

Team Member of:

My research has focused on the regulated destruction of proteins by the ubiquitin–proteasome pathway. As a structural biologist, I have been working on exploring the ubiquitin–proteasome system at the atomic level by using biophysical techniques such as NMR. I have solved the structures of ubiquitin receptors complex with various ubiquitin species or their preferred shuttle factors. I’m also directing my research interest towards expanding my skillset to study larger proteasome complexes that may not be tractable by NMR, by merging my protein chemistry and NMR skills with new skills. One of my jobs is to training and assisting my co-workers by sharing my skill set.
Areas of Expertise

1) NMR spectroscopy, 2) structural biology, 3) computer modeling of protein structures and complexes, 4) protein engineering and isolation

Contact Info

Xiang Chen, Ph.D.
Center for Cancer Research
National Cancer Institute
Bldg. 538, Room 166
Frederick, MD 21702-1201
Ph: 301-846-7396
Dr. Chen’s longstanding research interest is studying the mechanism of how the proteasome recognizes and processes its substrate by using various biophysical techniques, including NMR, mass spectroscopy, and computer modeling. The ubiquitin-proteasome pathway is important to human health as this process regulates protein quality control, transcription, apoptosis, DNA repair, immune response and cell cycle control. The malfunction of the ubiquitin-proteasome pathway is associated with neurological disorders, inflammatory processes and cancer. By using NMR, he has contributed to the discovery of Rpn13 and Rpn1 as substrate receptors in the proteasome. Ubiquitin chains of diverse linkage type can be used to signal for protein degradation by the proteasome and he has dissected the ubiquitin chain preferences of these ubiquitin receptors by using pull-down assays. He has solved the structure of each of these ubiquitin receptors in complex with various ubiquitin species or their preferred shuttle factors. Moreover, he has used NMR and mass spectroscopy to study how small molecules interact with hRpn13 to inhibit proteasome function.
NIH Scientific Focus Areas:
Structural Biology

Selected Key Publications

  1. Chen X.*, Randles L.*, Shi K., Tarasov, S.G., Aihara, H., Walters K.J. (Co-first author)
    Structure. 24: 1257-1270, 2016. [ Journal Article ]
  2. Shi Y.*, Chen X.*, Elsasser S.*, Stocks B.*, Tian G., Lee B.H., Shi Y.H., Zhang N.X., de Poot S.A.H., Tuebing F., Sun S.W., Vannoy J., Tarasov S.G., Engen J.R., Finley D., Walters K.J. (Co-first author)
    Science. 351: In press, 2016. [ Journal Article ]
  3. Chen X., Lee B.H., Finley D., Walters K.J.
    Mol Cell. 38: 404-415, 2010. [ Journal Article ]
  4. Chen X., Solomon W.C., Kang Y., Cerda-Maira F., Darwin K.H., Walters K.J.
    J Mol Biol. 392: 208-217, 2009. [ Journal Article ]
  5. Schreiner P.*, Chen X.*, Husnjak K.*, Randles L., Zhang N.X., Elsasser S., Finley D., Dikic I., Walters K.J., Groll M. (Co-first author)
    Nature. 453: 548-552, 2008. [ Journal Article ]
Dr. Chen received his Ph. D. from Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences and his graduate work was about the structure/function studies of scorpion toxin peptides. In 2005, he joined Dr. Kylie Walters’ group at University of Minnesota as a postdoctoral associate, and started to study the ubiquitin-proteasome system by using NMR. In 2013 he moved to Maryland and became a research fellow in Protein Processing Section, Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute. In 2016, he was promoted to be a Staff Scientist.