Lixin Fan, Ph.D.

Lixin Fan, Ph.D.
Senior Scientist, Leidos Biomedical Research, Inc.
CCR SAXS Core Facility

Team Member of:

Dr. Lixin Fan contributes her expertise in small-angle scattering to the user community of the CCR SAXS Core Facility scientifically and technically. Dr. Fan plays a major role in designing and conducting SAXS/WAXS experiments, data collection, processing, analysis/modeling and interpretation for multi-disciplinary, multi-laboratory collaborations. Dr. Fan also maintains and operates the in-house SAXS instrument, runs necessary equipment at the Advanced Photon Source of Argonne National Laboratory and trains users to operate both the Argonne beamline and the in-house instrument.

Areas of Expertise

1) structural characterization of biomacromolecules and their complexes and soft nanomaterials, 2) small-angle X-ray and neutron scattering, 3) fluctuation X-ray microscopy, 4) coherent X-ray scattering, 5) grazing-incidence small-angle scattering transmission/fluorescence scanning X-ray microscopy, 6) X-ray phase contrast imaging

Contact Info

Lixin Fan, Ph.D.
Center for Cancer Research
National Cancer Institute
Bldg. 538/Room 119
Frederick, MD 21701
Ph: 301-846-1362

The CCR SAXS Core Facility supports the research projects of CCR principal investigators (PIs), NIH intramural PIs, and extramural academic research groups/laboratories. Support includes providing access to an in-house, state-of-the-art SAXS instrument (BioSAXS-2000) and routine access to the Synchrotron SAXS/WAXS instrument at the 12 ID-B beamline of the Advanced Photon Source, Argonne National Laboratory, through a Partner User Program. The SAXS Core also provides expertise in experimental design, data collection, processing, analysis and interpretation. The main focus of the research is to determine the structure of biomacromolecules and their complexes in solution. The research field includes, but is not limited to, structural studies of nucleic acids, proteins, protein assemblies, virus particles, lipid membranes, protein/DNA and protein/RNA complexes.


NIH Scientific Focus Areas:
Structural Biology

Selected Key Publications

  1. Zhang Q, Fan L, Hou F, Dong A, Wang YX, and Tong Y.
    Sci Rep. 5: 15660, 2015. [ Journal Article ]
  2. The absolute calibration of a small-angle scattering instrument with a laboratory X-ray source.
    Fan L, Degen M, Bendle S, Grupido N, and Ilavsky J.
    Journal of Physics. 247: 012005, 2010. [ Journal Article ]
  3. Fan F, Paterson D, McNulty I, Treacy MMJ, and Gibson JM.
    J Microsc. 225: 41, 2007. [ Journal Article ]
  4. Structural studies of the 30S subunit of ribosomes Thermus thermophilus by small-angle neutron and X-ray scattering.
    Fan L, Svergun DI, Volkov VV, Aksenov VL, Algalarov CCh, Selivanova OM, Shcherbakova IV, Koch M, Gilles R, Wiedenmann A, May R, and Serdyuk IN.
    J Appl Crystallogr. 33: 515, 2000. [ Journal Article ]
  5. Determination of deuterium incorporation into RNA and protein components of the E.coli ribosome at biosynthetic deuteration by small-angle neutron scattering.
    Fan L, Zgurskaya EI, and Serdyuk IN.
    J Appl Crystallogr. 30: 59, 1997. [ Journal Article ]

Dr. Lixin Fan obtained her Ph.D. in physics and mathematics from the Joint Institute for Nuclear Research (Dubna, Moscow region, Russia) and the Institute of Protein Research, Russian Academy of Sciences (Puschino, Moscow region, Russia) where she used small-angle neutron and X-ray scattering (SANS and SAXS) to study the structure of the 30S ribosomal subunit from Thermus thermophilus. From 2000-2002, as a Postdoctoral Fellow at the Intense Pulsed Neutron Source Division of Argonne National Laboratory (ANL), she studied the block copolymer self-assembly and phase transition using SAXS and SANS techniques. From 2002-2007, she worked at the Advanced Photon Source Division of ANL where she served the user community and developed a novel technique known as “fluctuation X-ray microscopy" based on coherent X-ray scattering and X-ray scanning probe imaging techniques and applied this technique for studying medium-range order in disordered materials. Before she joined the CCR SAXS Core Facility, Dr. Fan worked as an applications scientist at Rigaku Innovative Technologies, where she trained researchers worldwide in using the SAXS technique, developed in-house SAXS/GISAXS instrument and applications, characterized a wide range of nanomaterials using SAXS/WAXS, USAXS and GISAXS techniques. As a SAXS expert and a member of the U.S. delegation, she contributed to ISO standard ISO/FDIS 17867 «Particle Size Analysis – Small-Angle X-Ray Scattering». Dr. Fan joined the CCR SAXS Core Facility in 2014, where she serves the NCI and NIH intramural and extramural user community and conducts research focusing on the structural characterization of biomacromolecules and their complexes.