Alfred Singer, M.D.
Chief
Head, Lymphocyte Development Section

Center for Cancer Research
National Cancer Institute

Building 10, Room 4B36
Bethesda, MD 20892
301-496-5461

Dr. Singer and his team seek to elucidate the molecular mechanism by which thymic selection generates an MHC-restricted T cell receptor repertoire that is coordinated with CD4 and CD8 coreceptor expression and appropriate T cell function.

Areas of Expertise
1) lymphocyte development, 2) immunology

Lymphocyte Development

The differentiation of immature CD4+8+ (double positive, DP) thymocytes into mature T cells is the key event in T cell development as it gives rise to functional T cells and determines the mature T cell repertoire. Our research seeks to elucidate the molecular mechanism by which thymic selection generates an MHC-restricted T cell receptor repertoire that is coordinated with CD4 and CD8 coreceptor expression and appropriate T cell function.

Scientific Focus Areas:
Developmental Biology, Immunology
Selected Recent Publications
  1. Tai X, Singer A.
    Cell Cycle. 13: 501-2, 2014. [ Journal Article ]
  2. Mu J, Tai X, Iyer SS, Weissman JD, Singer A, Singer DS.
    J. Immunol. 192: 2892-903, 2014. [ Journal Article ]
  3. Tai X, Erman B, Alag A, Mu J, Kimura M, Katz G, Guinter T, McCaughtry T, Etzensperger R, Feigenbaum L, Singer DS, Singer A.
    Immunity. 38: 1116-28, 2013. [ Journal Article ]
  4. Kimura MY, Pobezinsky LA, Guinter TI, Thomas J, Adams A, Park JH, Tai X, Singer A.
    Nat. Immunol. 14: 143-51, 2013. [ Journal Article ]
  5. Van Laethem F, Tikhonova AN, Pobezinsky LA, Tai X, Kimura MY, Le Saout C, Guinter TI, Adams A, Sharrow SO, Bernhardt G, Feigenbaum L, Singer A.
    Cell. 154: 1326-41, 2013. [ Journal Article ]

Dr. Singer received his M.D. from Columbia University and his clinical training at the Columbia-Presbyterian Medical Center. He was a fellow in immunology at the Rockefeller University before coming to the NCI. He is chief of the Experimental Immunology Branch and his research interests are in lymphocyte recognition and development, especially the molecular and cellular recognition signals that result in self/nonself discrimination during early development.

Name Position
Amala S. Alag Ph.D. Research Biologist
Abhisek Bhattacharya Ph.D. Postdoctoral Fellow (CRTA)
Ruth Etzensperger Ph.D. Postdoctoral Fellow (CRTA)
Terry I. Guinter Research Biologist
Jeffrey A. Hammer Research Biologist
Tejas B. Kadakia Ph.D. Postdoctoral Fellow (Visiting)
Motoko Kimura Ph.D. Research Fellow
Michael J. Kruhlak Ph.D. Facility Head
Leonid A. Pobezinsky Ph.D. Research Fellow
Ingrid Saba Ph.D. Postdoctoral Fellow (CRTA)
Miho Shinzawa Ph.D. Postdoctoral Fellow (Visiting)
Xuguang Tai Ph.D. Staff Scientist
Julien Thomas Predoctoral Fellow
Francois Van Laethem Ph.D. Staff Scientist

Summary

Dr. Singer and his team seek to elucidate the molecular mechanism by which thymic selection generates an MHC-restricted T cell receptor repertoire that is coordinated with CD4 and CD8 coreceptor expression and appropriate T cell function.

Areas of Expertise
1) lymphocyte development, 2) immunology

Research

Lymphocyte Development

The differentiation of immature CD4+8+ (double positive, DP) thymocytes into mature T cells is the key event in T cell development as it gives rise to functional T cells and determines the mature T cell repertoire. Our research seeks to elucidate the molecular mechanism by which thymic selection generates an MHC-restricted T cell receptor repertoire that is coordinated with CD4 and CD8 coreceptor expression and appropriate T cell function.

Scientific Focus Areas:
Developmental Biology, Immunology

Publications

Selected Recent Publications
  1. Tai X, Singer A.
    Cell Cycle. 13: 501-2, 2014. [ Journal Article ]
  2. Mu J, Tai X, Iyer SS, Weissman JD, Singer A, Singer DS.
    J. Immunol. 192: 2892-903, 2014. [ Journal Article ]
  3. Tai X, Erman B, Alag A, Mu J, Kimura M, Katz G, Guinter T, McCaughtry T, Etzensperger R, Feigenbaum L, Singer DS, Singer A.
    Immunity. 38: 1116-28, 2013. [ Journal Article ]
  4. Kimura MY, Pobezinsky LA, Guinter TI, Thomas J, Adams A, Park JH, Tai X, Singer A.
    Nat. Immunol. 14: 143-51, 2013. [ Journal Article ]
  5. Van Laethem F, Tikhonova AN, Pobezinsky LA, Tai X, Kimura MY, Le Saout C, Guinter TI, Adams A, Sharrow SO, Bernhardt G, Feigenbaum L, Singer A.
    Cell. 154: 1326-41, 2013. [ Journal Article ]

Biography

Dr. Singer received his M.D. from Columbia University and his clinical training at the Columbia-Presbyterian Medical Center. He was a fellow in immunology at the Rockefeller University before coming to the NCI. He is chief of the Experimental Immunology Branch and his research interests are in lymphocyte recognition and development, especially the molecular and cellular recognition signals that result in self/nonself discrimination during early development.

Team

Name Position
Amala S. Alag Ph.D. Research Biologist
Abhisek Bhattacharya Ph.D. Postdoctoral Fellow (CRTA)
Ruth Etzensperger Ph.D. Postdoctoral Fellow (CRTA)
Terry I. Guinter Research Biologist
Jeffrey A. Hammer Research Biologist
Tejas B. Kadakia Ph.D. Postdoctoral Fellow (Visiting)
Motoko Kimura Ph.D. Research Fellow
Michael J. Kruhlak Ph.D. Facility Head
Leonid A. Pobezinsky Ph.D. Research Fellow
Ingrid Saba Ph.D. Postdoctoral Fellow (CRTA)
Miho Shinzawa Ph.D. Postdoctoral Fellow (Visiting)
Xuguang Tai Ph.D. Staff Scientist
Julien Thomas Predoctoral Fellow
Francois Van Laethem Ph.D. Staff Scientist