Wendy D. DuBois

Facility Head
Head, Animal Model and Genotyping Core Facility

The LCBG Animal Model and Genotyping Core Facility provides outstanding, reliable research support to a number of NCI intramural investigators. The Facility's mission is to create and maintain mouse models for various cancers including: - B cell lymphomas, - plasma cell tumors, - myeloma, - skin carcinogenesis and melanoma, - breast cancer, and - prostate cancer. The Core also performs a variety of genomic analyses in support of investigators.

Areas of Expertise
genotyping, experiment design, colony management

Contact Info

Wendy D. DuBois
Center for Cancer Research
National Cancer Institute
Building 28, Room T105
Bethesda, MD 20892-4255
Ph: 240-760-6090
240-760-6047
duboiswe@mail.nih.gov

The LCBG Animal Model and Genotyping Core Facility provides outstanding, reliable research support to a number of NCI intramural investigators. The Facility's mission is to create and maintain mouse models for various cancers including: - B cell lymphomas, - plasma cell tumors, - myeloma, - skin carcinogenesis and melanoma, - breast cancer, and - prostate cancer. The Core also performs a variety of genomic analyses in support of investigators. We continually search for ways to make the techniques we use every day more efficient by keeping up with new technologies, software, and equipment. Facility staff members are responsible for ensuring the genetic integrity and overseeing all of the breeding strategies required to establish and maintain many different lines of mice. Our staff conduct all of the genetic monitoring and genotyping required to maintain these lines, including: - DNA extraction - genotyping by PCR - gel electrophoresis

The Core actively participates in planning, design, execution, and analysis of tumor induction experiments and sets up and executes preclinical testing of various drugs for anti-tumor activity. Facility staff members write many of the original Animal Study Proposals and associated amendments, and ensure that these documents are kept up-to-date and in compliance with the NCI Animal Care and Use Committee (ACUC). We actively participate in preparing for all inspections by the ACUC and the Association for Assessment and Accreditation of Laboratory Animal Care (AALAC). The Core is also responsible for coordinating the shipment of mouse and tumor lines to investigators around the world, thereby providing useful research tools to the extramural cancer research community.

The Facility's Genomics Section provides assistance both to LCBG investigators and to other CCR investigators as appropriate, for a wide variety of genomic applications including, but not limited to: - database mining, - marker development, - single nucleotide polymorphism (SNP) identification and analysis, and - sequencing and sequence homology searches. Programs currently supported by the Core include MacVector, DS Gene, Vector NTI, LaserGene, Sequencher, Partek, and Ingenuity Pathway Analysis. The strong combination of animal and genomic expertise within the Core provides integrated assistance to CCR investigators in their mechanistic studies of cancer initiation, progression, and metastasis.

Scientific Focus Areas:
Cancer Biology, Genetics and Genomics

Selected Publications

  1. Wu JJ, Liu J, Chen EB, Wang JJ, Cao L, Narayan N, Fergusson MM, Rovira II, Allen M, Springer DA, Lago CU, Zhang S, DuBois W, Ward T, deCabo R, Gavrilova O, Mock B, Finkel T.
    Cell Rep. 4: 913-20, 2013. [ Journal Article ]
  2. He Y, Zhu W, Shin MH, Gary J, Liu C, Dubois W, Hoover SB, Jiang S, Marrogi E, Mock B, Simpson RM, Huang J.
    Stem Cell Reports. June 6: 1630-1644, 2017. [ Journal Article ]
  3. Simmons JK, Michalowski AM, Gamache BJ, DuBois W, Patel J, Zhang K, Gary J, Zhang S, Gaikwad S, Connors D, Watson N, Leon E, Chen JQ, Kuehl WM, Lee MP, Zingone A, Landgren O, Ordentlich P, Huang J, Mock BA.
    Mol Cancer Ther . May 18: 2017. [ Journal Article ]
  4. Cataisson C, Michalowski AM, Shibuya K, Ryscavage A, Klosterman M, Wright L, Dubois W, Liu F, Zhuang A, Rodrigues KB, Hoover S, Dwyer J, Simpson MR, Merlino G, Yuspa SH.
    Sci Signal. Jun 21:9(433): 2016. [ Journal Article ]
  5. Qian J, Wang Q, Dose M, Pruett N, Kieffer-Kwon KR, Resch W, Liang G, Tang Z, Mathé E, Benner C, Dubois W, Nelson S, Vian L, Oliveira TY, Jankovic M, Hakim O, Gazumyan A, Pavri R, Awasthi P, Song B, Liu G, Chen L, Zhu S, Feigenbaum L, Staudt L, Murre C, Ruan Y, Robbiani DF, Pan-Hammarström Q, Nussenzweig MC, Casellas R.
    Cell. Dec18;159(7): 1524-37, 2014. [ Journal Article ]
Name Position
Mario A. Anzano Biological Laboratory Technician
William Beals Postbaccalaureate Fellow (IRTA/CRTA)
Marta Custer Animal Technician (Contr)
Hao T. Du Guest Researcher
Maudeline Etienne Animal Technician (Contr)
Anthony Vieira Biological Laboratory Technician
Susan Walters Animal Technician (Contr)
Tina Wellington Biological Laboratory Technician