Ravi B. Chalamalasetty, Ph.D.
Research in cancer biology and developmental biology have greatly benefitted from each other’s progress over the past few decades. A closer look reveals that many of the molecular and cellular processes essential for the normal embryonic development are mis-regulated in cancer progression. In our lab, we are specifically interested in understanding the molecular mechanisms that regulate tissue homeostasis. A delicate balance of stem cell self-renewal and differentiation is required for the maintenance of normal embryonic structures, adult organogenesis, and wound repair. However, the molecular, genetic and cellular cues essential for this biological process are not very clear. We are utilizing a variety of approaches from molecular biology, mouse genetics, embryonic development, embryonic stem cell differentiation, functional genomics and bioinformatics to address these questions.
1) embryonic development, 2) embryonic stem cells, 3) mouse genetics, 4) genomics,
5) Wnt signaling, 6) transcription factors
Center for Cancer Research
National Cancer Institute
Building 539, Room 205
Frederick, MD 21702-1201
My research centers on understanding molecular mechanisms that govern stem cell self-renewal and differentiation. Wnts are stem cell factors essential for normal embryonic development, differentiation, migration and specification of mesodermal progenitors. Wnts bind to receptors to relay signal transduction via b-catenin to activate down stream target genes. Over the past few years I have taken molecular, genetic and genomic approaches to identify and characterize Wnt target genes. My main research interests are: (1) To understand how Wnts regulate self-renewal of stem cells and at the same time essential for cell-fate determination and differentiation. (2) To understand the molecular cross-talk of Wnt signals with other signaling pathways such as FGF, Notch-Delta and BMP signaling during embryonic development and cell fate determination. (3) To delineate gene-regulatory network by identifying molecular mechanisms at the level of Wnt regulated transcription factors. (4) To understand the molecular mechanisms of developmentally regulated genes in cancer initiation and progression.
Selected Key Publications
Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription.Proc Natl Acad Sci U S A. Epub ahead of print, 2016. [ Journal Article ]
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.Development. 142(9): 1628-38, 2015. [ Journal Article ]
- Development. 141(22): 4285-97, 2014. [ Journal Article ]
The Wnt3a/β-catenin target gene Mesogenin1 controls the segmentation clock by activating a Notch signalling program.Nat Commun. 2: 390, 2011. [ Journal Article ]
Wnt3a/beta-catenin signaling controls posterior body development by coordinating mesoderm formation and segmentation.Development. 135(1): 85-94, 2008. [ Journal Article ]
Dr. Ravi Chalamalasetty obtained his Ph.D. in cell biology in the laboratory of Prof. Dr. Erich A. Nigg at Max Planck Institute for Biochemistry in Munich, Germany in 2006. Dr. Chalamalasetty studied the mechanisms of mammalian cytokinesis and was mentored by Dr. Erich A. Nigg and Dr. Herman Sillje. He received the Max Planck Fellowship for Doctoral Students from 2002-2006. For postdoctoral studies, he joined the Cancer and Developmental Biology Laboratory at the National Cancer Institute at Frederick in Dr. Terry Yamaguchi’s lab as a visiting fellow. Since 2015, he has served as a Staff Scientist at the National Cancer Institute at Frederick.