Zbigniew  Dauter, Ph.D.
Zbigniew Dauter, Ph.D.
Senior Investigator
Head, Synchrotron Radiation Research Section

Center for Cancer Research
National Cancer Institute

Bldg. 202, Rm. Q142
Argonne, IL 60439
630-252-3960

Dr. Dauter and his Synchrotron Radiation Research Section of MCL are located at the Argonne National Laboratory, the site of the largest U.S. synchrotron facility. He uses X-ray diffraction technique to solve crystal structures of various proteins and nucleic acids of biological and medical relevance. He is specializing in analyzing crystal structures at extremely high resolution and accuracy and in developing methods of effective diffraction data collection and in using weak anomalous dispersion effects to solve structures of macromolecules.

Areas of Expertise
1) structural biology 2) macromolecular crystallography 3) diffraction data collection 4) use of anomalous diffraction for phasing

The Synchrotron Radiation Research Section is located at the Argonne National Laboratory near Chicago, Illinois. The aim of the research within this Section is to utilize the unique properties of the X-ray radiation generated at synchrotron storage rings-in particular, at the Southeast Regional Collaborative Access Team (SERCAT) beamlines of the Advanced Photon Source (APS).

The two most important characteristics of synchrotron radiation are the high intensity of the X-ray beam and the tunability of the wavelength; both of these properties are utilized in the research investigations within the Section. The SERCAT beamlines (based on the undulator and the bending magnet) are easily tunable within the wavelength range from beyond the bromine absorption edge to long wavelengths, and successful MAD experiments have been performed on such anomalous scatterers as bromine, selenium, mercury, platinum, zinc, holmium, and iron. On the other hand, long wavelengths permit us to utilize the very weak anomalous signal of lighter elements, such as sulfur and phosphorus, naturally existing in proteins and nucleic acids. Several atomic-resolution diffraction data sets extending to ultra-high resolution (beyond 0.8 angstrom) have been collected and used for precise model refinements.

In addition to conducting research investigations, the Section personnel provide technological and scientific support for NIH researchers collecting diffraction data at APS. The NIH Intramural Synchrotron Consortium of macromolecular crystallographers is a part of SERCAT and uses about 20% of the total available time at SERCAT beamlines. Collecting diffraction data at the synchrotron beamline involves a very high level of technology--but it is a scientific process, not a technicality. Therefore, a proper selection of all the necessary parameters can be done only if based on scientific considerations.

Scientific Focus Areas:
Biomedical Engineering and Biophysics, Structural Biology
Selected Key Publications
  1. Brzozowski AM, Pike AC, Dauter Z, Hubbard RE, Bonn T, Engström O, Ohman L, Greene GL, Gustafsson JA, Carlquist M.
    Nature. 389: 753-8, 1997. [ Journal Article ]
  2. Wang J, Dauter M, Alkire R, Joachimiak A, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 63: 1254-68, 2007. [ Journal Article ]
  3. Jedrzejczak R, Wang J, Dauter M, Szczesny RJ, Stepien PP, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 67: 988-96, 2011. [ Journal Article ]
  4. Brzezinski K, Brzuszkiewicz A, Dauter M, Kubicki M, Jaskolski M, Dauter Z.
    Nucleic Acids Res. 39: 6238-48, 2011. [ Journal Article ]
  5. Rajashankar K, Dauter Z.
    Methods Mol. Biol. 1140: 211-37, 2014. [ Journal Article ]

Dr. Dauter earned his Ph.D. in Crystallography from the Technical University of Gdansk in 1975 under the direction of Professor Z. Kosturkiewicz. From 1975 to 1997, he conducted research in structural crystallography at the University of York, European Molecular Biology Laboratory in Hamburg, and Technical University of Gdansk. He established the Synchrotron Radiation Research Section at Brookhaven National Laboratory (BNL) in late 1997, and joined the Macromolecular Crystallography Laboratory, NCI, as a Section Chief in 2000. His laboratory was relocated from BNL to Argonne National Laboratory at the end of 2004.

Name Position
Miroslawa Dauter Senior Research Assistant (Contr)
Zhipu Luo Ph.D. Postdoctoral Fellow (Visiting)
Sergei Pletnev Ph.D. Staff Scientist (Contr)
Milosz Ruszkowski Postdoctoral Fellow (Visiting)

Summary

Dr. Dauter and his Synchrotron Radiation Research Section of MCL are located at the Argonne National Laboratory, the site of the largest U.S. synchrotron facility. He uses X-ray diffraction technique to solve crystal structures of various proteins and nucleic acids of biological and medical relevance. He is specializing in analyzing crystal structures at extremely high resolution and accuracy and in developing methods of effective diffraction data collection and in using weak anomalous dispersion effects to solve structures of macromolecules.

Areas of Expertise
1) structural biology 2) macromolecular crystallography 3) diffraction data collection 4) use of anomalous diffraction for phasing

Research

The Synchrotron Radiation Research Section is located at the Argonne National Laboratory near Chicago, Illinois. The aim of the research within this Section is to utilize the unique properties of the X-ray radiation generated at synchrotron storage rings-in particular, at the Southeast Regional Collaborative Access Team (SERCAT) beamlines of the Advanced Photon Source (APS).

The two most important characteristics of synchrotron radiation are the high intensity of the X-ray beam and the tunability of the wavelength; both of these properties are utilized in the research investigations within the Section. The SERCAT beamlines (based on the undulator and the bending magnet) are easily tunable within the wavelength range from beyond the bromine absorption edge to long wavelengths, and successful MAD experiments have been performed on such anomalous scatterers as bromine, selenium, mercury, platinum, zinc, holmium, and iron. On the other hand, long wavelengths permit us to utilize the very weak anomalous signal of lighter elements, such as sulfur and phosphorus, naturally existing in proteins and nucleic acids. Several atomic-resolution diffraction data sets extending to ultra-high resolution (beyond 0.8 angstrom) have been collected and used for precise model refinements.

In addition to conducting research investigations, the Section personnel provide technological and scientific support for NIH researchers collecting diffraction data at APS. The NIH Intramural Synchrotron Consortium of macromolecular crystallographers is a part of SERCAT and uses about 20% of the total available time at SERCAT beamlines. Collecting diffraction data at the synchrotron beamline involves a very high level of technology--but it is a scientific process, not a technicality. Therefore, a proper selection of all the necessary parameters can be done only if based on scientific considerations.

Scientific Focus Areas:
Biomedical Engineering and Biophysics, Structural Biology

Publications

Selected Key Publications
  1. Brzozowski AM, Pike AC, Dauter Z, Hubbard RE, Bonn T, Engström O, Ohman L, Greene GL, Gustafsson JA, Carlquist M.
    Nature. 389: 753-8, 1997. [ Journal Article ]
  2. Wang J, Dauter M, Alkire R, Joachimiak A, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 63: 1254-68, 2007. [ Journal Article ]
  3. Jedrzejczak R, Wang J, Dauter M, Szczesny RJ, Stepien PP, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 67: 988-96, 2011. [ Journal Article ]
  4. Brzezinski K, Brzuszkiewicz A, Dauter M, Kubicki M, Jaskolski M, Dauter Z.
    Nucleic Acids Res. 39: 6238-48, 2011. [ Journal Article ]
  5. Rajashankar K, Dauter Z.
    Methods Mol. Biol. 1140: 211-37, 2014. [ Journal Article ]

Biography

Dr. Dauter earned his Ph.D. in Crystallography from the Technical University of Gdansk in 1975 under the direction of Professor Z. Kosturkiewicz. From 1975 to 1997, he conducted research in structural crystallography at the University of York, European Molecular Biology Laboratory in Hamburg, and Technical University of Gdansk. He established the Synchrotron Radiation Research Section at Brookhaven National Laboratory (BNL) in late 1997, and joined the Macromolecular Crystallography Laboratory, NCI, as a Section Chief in 2000. His laboratory was relocated from BNL to Argonne National Laboratory at the end of 2004.

Team

Name Position
Miroslawa Dauter Senior Research Assistant (Contr)
Zhipu Luo Ph.D. Postdoctoral Fellow (Visiting)
Sergei Pletnev Ph.D. Staff Scientist (Contr)
Milosz Ruszkowski Postdoctoral Fellow (Visiting)