Thomas A. Johnson headshot

Thomas A. Johnson, M.S.

  • Center for Cancer Research
  • National Cancer Institute


My research focuses on how chromatin and transcription factor dynamics control gene expression with an emphasis on steroid hormone receptor biology. My research includes the use of genome-wide chromatin accessibility, chromatin structure, gene expression and transcription factor binding techniques in combination with cutting-edge microscopy to study the mechanisms of gene regulation. My current studies are exploring how 3-dimensional nuclear architecture, enhancer organization, and nuclear receptor interactions control gene expression.

Areas of Expertise

Transcription Regulation
Chromatin Structure
Nuclear Receptor Biology
Genomic Analyses
Single Molecule Tracking


Selected Recent Publications

Genome-wide binding potential and regulatory activity of the glucocorticoid receptor’s monomeric and dimeric forms.

Thomas A. Johnson, Ville Paakinaho, Sohyoung Kim, Gordon L. Hager & Diego M. Presman.
Nature Communications. 12: 1987, 2021. [ Journal Article ]

Glucocorticoid receptor quaternary structure drives chromatin occupancy and transcriptional outcome.

Thomas A. Johnson, Ville Paakinaho, Diego M. Presman, Gordon L. Hager.
Genome Research. 29: 1223–1234, 2019. [ Journal Article ]

An intrinsically disordered region-mediated confinement state contributes to the dynamics and function of transcription factors.

David A. Garcia, Thomas A. Johnson, Diego M. Presman, Gregory Fettweis, Kaustubh Wagh, Lorenzo Rinaldi, Diana A. Stavreva, Ville Paakinaho, Rikke A.M. Jensen, Susanne Mandrup, Arpita Upadhyaya, Gordon L. Hager.
Molecular Cell. 81: 1-15, 2021. [ Journal Article ]

Conventional and pioneer modes of glucocorticoid receptor interaction with enhancer chromatin in vivo.

Thomas A. Johnson, Razvan V. Chereji, Diana A. Stavreva, Stephanie A. Morris, Gordon L. Hager, David J. Clark.
Nucleic Acids Research. 46 (1): 203-214 , 2018. [ Journal Article ]