Breadcrumb

Jeffrey N. Strathern, Ph.D.

Jeffrey N. Strathern, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
RNA Biology Laboratory

RESEARCH SUMMARY

Two general areas of research were pursued in our section: mechanisms of genetic recombination and the fidelity of transcription. We focused on the area of genetic recombination in general and double-strand-break repair in particular. DNA palindromes are thought to be a common mechanism of gene amplification found in tumor cells. However, palindromes are particularly difficult to investigate because they are difficult to clone and difficult to sequence. We created yeast strains that tolerate DNA palindromes and developed a system in which they can be generated and we also developed a method to determine their sequence.

Areas of Expertise

1) genetic recombination 2) gene regulation

Publications

Selected Recent Publications

Intrinsic translocation barrier as an initial step in pausing by RNA polymerase II

Imashimizu M, Kireeva ML, Lubkowska L, Gotte D, Parks AR, Strathern JN, Kashlev M.
J. Mol. Biol. 425: 697-712, 2013. [ Journal Article ]

Isolation and characterization of RNA polymerase rpoB mutations that alter transcription slippage during elongation in Escherichia coli

Zhou YN, Lubkowska L, Hui M, Court C, Chen S, Court DL, Strathern J, Jin DJ, Kashlev M.
J. Biol. Chem. 288: 2700-10, 2013. [ Journal Article ]

The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae

Strathern J, Malagon F, Irvin J, Gotte D, Shafer B, Kireeva M, Lubkowska L, Jin DJ, Kashlev M.
J. Biol. Chem. 288: 2689-99, 2013. [ Journal Article ]

Isolation and characterization of transcription fidelity mutants

Strathern JN, Jin DJ, Court DL, Kashlev M.
Biochim Biophys Acta B. 1819: 694-9, 2012. [ Journal Article ]

Mechanism of translesion transcription by RNA polymerase II and its role in cellular resistance to DNA damage

Walmacq C, Cheung AC, Kireeva ML, Lubkowska L, Ye C, Gotte D, Strathern JN, Carell T, Cramer P, Kashlev M.
Mol. Cell. 46: 18-29, 2012. [ Journal Article ]