Fedor Kouzine, Ph.D.

Fedor Kouzine, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
Laboratory of Pathology


Dr. Kouzine focuses on the understanding of how mechanical forces generated by genetic processes modify DNA and chromatin structure to regulate genes in health and disease. His work showed that in living cells torque generated during transcription modifies DNA structure dynamically at particular supercoil-sensitive spots in the genome. These unusual DNA structures then bind by specialized factors to feedback regulate gene expression. This work has important implications for the understanding and treatment of human diseases such as cancer. 

Areas of Expertise

1) epigenetics and nuclear architecture, 2) DNA topology and topoisomerases,
3) gene expression


Selected Key Publications

Permanganate/S1 Nuclease Footprinting Reveals Non-B DNA Structures with Regulatory Potential across a Mammalian Genome

Kouzine F, Wojtowicz D, Baranello L, Yamane A, Nelson S, Resch W, Kieffer-Kwon KR, Benham CJ, Casellas R, Przytycka TM, and Levens D.
Cell Systems. 4(3): 344-356, 2017. [ Journal Article ]

Global regulation of promoter melting in naive lymphocytes

Kouzine F, Wojtowicz D, Yamane A, Resch W, Kieffer-Kwon KR, Bandle R, Nelson S, Nakahashi H, Awasthi P, Feigenbaum L, Menoni H, Hoeijmakers J, Vermeulen W, Ge H, Przytycka TM, Levens D, and Casellas R.
Cell. 153(5): 988-99, 2013. [ Journal Article ]

Transcription-dependent dynamic supercoiling is a short-range genomic force

Kouzine F, Gupta A, Baranello L, Wojtowicz D, Ben-Aissa K, Liu J, Przytycka TM, and Levens D.
Nat Struct Mol Biol. 20(3): 396-403, 2013. [ Journal Article ]