Breadcrumb

Daniël P. Melters, Ph.D.

Daniël P. Melters, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute

RESEARCH SUMMARY

How are chromatin domains formed and how are they maintained? How can a chromatin domains exist in both a diffuse state and as a discrete locus while maintaining it's well-defined function? How do histone variants, in combination with nucleosome-binding proteins, orchestrate the functional aspects of the chromatin fiber? The centromere, defined by centromere-specific proteins such as the histone variant CENP-A, is an ideal model system, as its genomic organization is dramatically different between species. Budding yeast has point centromeres, humans have regional centromeres, whereas nematodes are holocentric. Nevertheless, the role of centromeric chromatin remains the same. Using biochemical and biphysical tools, Dr. Melters dissects how chromatin accessibility is modulated by histone variants and chromatin-binding factors.

Areas of Expertise

1) chromatin biology, 2) centromere, 3) genome evolution, 4) atomic force microscopy (AFM), 5) high-speed AFM

Publications

Selected Publications

Intrinsic elasticity of nucleosomes is encoded by histone variants and calibrated by their binding partners

Daniël P Melters , Mary Pitman , Tatini Rakshit , Emilios K Dimitriadis , Minh Bui , Garegin A Papoian , Yamini Dalall
Proc Natl Acad Sci U S A .. 116(46): 24066-24074, 2019. [ Journal Article ]

Chromatin Dynamics in Vivo: A Game of Musical Chairs

Daniël P Melters , Jonathan Nye , Haiqing Zhao , Yamini Dalal
Genes (Basel). 6(3): 751-76, 2015. [ Journal Article ]

Comparative analysis of tandem repeats from hundreds of species reveals unique insights into centromere evolution

Melters DP, Bradnam KR, Young HA, Telis N, May MR, Ruby JG, Sebra R, Peluso P, Eid J, Rank D, Garcia JF, DeRisi JL, Smith T, Tobias C, Ross-Ibarra J, Korf I, Chan SW.
Genome Biol. 14: R10, 2013. [ Journal Article ]

Holocentric chromosomes: convergent evolution, meiotic adaptations, and genomic analysis

Melters DP, Paliulis LV, Korf IF, and Chan SW.
Chromosome Res. 20: 579-93, 2012. [ Journal Article ]

Epithelial sodium channel regulated by differential composition of a signaling complex.

Soundararajan R, Melters D, Shih IC, Wang J, Pearce D.
Proc Natl Acad Sci USA. 106: 7804-9, 2009. [ Journal Article ]