Sergey G. Tarasov, Ph.D.
Sergey G. Tarasov, Ph.D.
Staff Scientist
Head, Biophysics Resource

Dr. Sergey G. Tarasov is overseeing the Biophysics Resource in the Structural Biophysics Laboratory, which provides CCR investigators with access to modern technologies and expertize in the area of biophysical chemistry. In this shared-use facility scientists get necessary training and perform their own experiments under supervision of expert staff, which provides technical or collaborative help with novel experimental design, data analysis and interpretation.

Areas of Expertise
biomolecular structure–property relationship, biomolecular interactions, biological thermodynamics, molecular spectroscopy, light scattering, liquid chromatography and mass spectrometry

Contact Info

Sergey G. Tarasov, Ph.D.
Center for Cancer Research
National Cancer Institute
Building 538, Room 172
Frederick, MD 21702-1201
301-846-1223
tarasovs@mail.nih.gov

The Biophysics Resource (BR) provides access to biophysical instrumentation and any required assistance for researchers from the Center for Cancer Research in need of characterization of systems subject to structural investigations. The BR offers and maintains cutting-edge biophysics technologies in the following areas: - Circular dichroism spectroscopy for studies of optical activity and conformation of biomacromolecules. - Steady-state fluorescence spectroscopy for studies of structure and environment of biomacromolecules and mechanism of their interactions. - Isothermal titration calorimetry for thermodynamic characterization of biomacromolecular interactions. - Liquid chromatography with mass spectrometry detection for macromolecular mass characterization. - Static and dynamic light scattering for determination of macromolecular mass, particle size and degree of aggregation - Microscale thermophoresis for macromolecular binding studies The BR operates as a shared-use facility. Users generally learn to operate the instruments and conduct their own experiments. The BR personnel train researchers wishing to use the facility and they are available to consult on experimental design/analysis or as collaborators in more detailed studies. Also, some equipment is too delicate to permit casual use, e.g. the high-sensitivity calorimeters, and users often collaborate with BR staff in the use of such instrumentation.

Selected Publications
  1. Tarasov SG, Gaponenko V, Howard OM, Chen Y, Oppenheim JJ, Dyba MA, Subramaniam S, Lee Y, Michejda C, Tarasova NI.
    Proc. Natl. Acad. Sci. U.S.A.. 108: 9798-803, 2011. [ Journal Article ]
  2. Tarasov SG, Casas-Finet JR, Cholody WM, Michejda CJ.
    Photochem Photobiol. 70: 568-78, 1999. [ Journal Article ]
  3. Tarasov SG, Casas-Finet JR, Cholody WM, Kosakowska-Cholody T, Gryczynski ZK, Michejda CJ.
    Photochem Photobiol. 78: 313-22, 2003. [ Journal Article ]
  4. Acchione M, Lipschultz CA, Desantis ME, Shanmuganathan A, Li M, Wlodawer A, Tarasov S, Smith-Gill SJ.
    Mol. Immunol.. 47: 457-64, 2009. [ Journal Article ]
  5. Khavrutskii L, Yeh J, Timofeeva O, Tarasov SG, Pritt S, Stefanisko K, Tarasova N.
    J Vis Exp. 2013. [ Journal Article ]

Sergey G Tarasov received M.S. degree in chemistry from Lomonosov Moscow State University (Russia) in 1975. He got his Ph.D.degree in chemistry of macromolecules, working on thermodynamics of multicomponent macromolecular systems in the laboratory of Prof. Yu.K.Godovski at the Karpov Institute of Physical Chemistry (Moscow, Russia). In 1997 he joined the Molecular Aspects of Drug Design section of ABL Research Program (head - Dr. C.J. Michejda) to study physico-chemical aspects of drug-DNA interactions. Since 2000 he is a Head of Biophysical Resource in the Structural Biophysics Laboratory (chief - Dr. R.A. Byrd)

Name Position
Marzena Dyba Research Assistant (Contr)

Research

The Biophysics Resource (BR) provides access to biophysical instrumentation and any required assistance for researchers from the Center for Cancer Research in need of characterization of systems subject to structural investigations. The BR offers and maintains cutting-edge biophysics technologies in the following areas: - Circular dichroism spectroscopy for studies of optical activity and conformation of biomacromolecules. - Steady-state fluorescence spectroscopy for studies of structure and environment of biomacromolecules and mechanism of their interactions. - Isothermal titration calorimetry for thermodynamic characterization of biomacromolecular interactions. - Liquid chromatography with mass spectrometry detection for macromolecular mass characterization. - Static and dynamic light scattering for determination of macromolecular mass, particle size and degree of aggregation - Microscale thermophoresis for macromolecular binding studies The BR operates as a shared-use facility. Users generally learn to operate the instruments and conduct their own experiments. The BR personnel train researchers wishing to use the facility and they are available to consult on experimental design/analysis or as collaborators in more detailed studies. Also, some equipment is too delicate to permit casual use, e.g. the high-sensitivity calorimeters, and users often collaborate with BR staff in the use of such instrumentation.

Publications

Selected Publications
  1. Tarasov SG, Gaponenko V, Howard OM, Chen Y, Oppenheim JJ, Dyba MA, Subramaniam S, Lee Y, Michejda C, Tarasova NI.
    Proc. Natl. Acad. Sci. U.S.A.. 108: 9798-803, 2011. [ Journal Article ]
  2. Tarasov SG, Casas-Finet JR, Cholody WM, Michejda CJ.
    Photochem Photobiol. 70: 568-78, 1999. [ Journal Article ]
  3. Tarasov SG, Casas-Finet JR, Cholody WM, Kosakowska-Cholody T, Gryczynski ZK, Michejda CJ.
    Photochem Photobiol. 78: 313-22, 2003. [ Journal Article ]
  4. Acchione M, Lipschultz CA, Desantis ME, Shanmuganathan A, Li M, Wlodawer A, Tarasov S, Smith-Gill SJ.
    Mol. Immunol.. 47: 457-64, 2009. [ Journal Article ]
  5. Khavrutskii L, Yeh J, Timofeeva O, Tarasov SG, Pritt S, Stefanisko K, Tarasova N.
    J Vis Exp. 2013. [ Journal Article ]

Biography

Sergey G Tarasov received M.S. degree in chemistry from Lomonosov Moscow State University (Russia) in 1975. He got his Ph.D.degree in chemistry of macromolecules, working on thermodynamics of multicomponent macromolecular systems in the laboratory of Prof. Yu.K.Godovski at the Karpov Institute of Physical Chemistry (Moscow, Russia). In 1997 he joined the Molecular Aspects of Drug Design section of ABL Research Program (head - Dr. C.J. Michejda) to study physico-chemical aspects of drug-DNA interactions. Since 2000 he is a Head of Biophysical Resource in the Structural Biophysics Laboratory (chief - Dr. R.A. Byrd)

Team

Name Position
Marzena Dyba Research Assistant (Contr)