illumina Cancer Exome
Narrowing the Field with Cancer Exome Sequencing
Many cancer causing variants are found within exonic, or protein coding regions, which comprise 1 to 2% of the genome. Cancer exome sequencing enables researchers to take a closer look at these regions.
Current exome sequencing approaches have severe limitations, with PCR and Sanger sequencing becoming cost-prohibitive when working with large regions and numbers of cancer samples. In addition, PCR demonstrates variable coverage between amplicons. Illumina has developed a number of solutions to overcome these challenges.
Sample Preparation for Cancer Exome Sequencing
Illumina's Nextera Rapid Capture Exome Kits are simple, cost-effective, all-in-one exome and sample preparation kits for use with HiSeq, MiSeq, or NextSeqsequencers. In addition, the Nextera Rapid Capture Expanded Exome version of these kits can detect regulatory regions as well as coding variants.
These kits are compatible with small quantities of DNA, allowing researchers to study scarce tumor samples that weren't previously possible to sequence. The streamlined exome sequencing workflows enable cancer researchers to identify coding variants in tumor samples up to 70% faster than with any other method.
