Sergey G. Tarasov, Ph.D.
Dr. Sergey G. Tarasov is overseeing the Biophysics Resource in the Structural Biophysics Laboratory, which provides CCR investigators with access to modern technologies and expertize in the area of biophysical chemistry. In this shared-use facility scientists get necessary training and perform their own experiments under supervision of expert staff, which provides technical or collaborative help with novel experimental design, data analysis and interpretation.
1) biomolecular structure–property relationship, 2) biomolecular interactions, 3) biological thermodynamics, 4) molecular spectroscopy, 5) light scattering, 6) liquid chromatography and mass spectrometry
The Biophysics Resource (BR) provides access to biophysical instrumentation and any required assistance for researchers from the Center for Cancer Research in need of characterization of systems subject to structural investigations. The BR offers and maintains cutting-edge biophysics technologies in the following areas: - Circular dichroism spectroscopy for studies of optical activity and conformation of biomacromolecules. - Steady-state fluorescence spectroscopy for studies of structure and environment of biomacromolecules and mechanism of their interactions. - Isothermal titration calorimetry for thermodynamic characterization of biomacromolecular interactions. - Liquid chromatography with mass spectrometry detection for macromolecular mass characterization. - Static and dynamic light scattering for determination of macromolecular mass, particle size and degree of aggregation - Microscale thermophoresis for macromolecular binding studies The BR operates as a shared-use facility. Users generally learn to operate the instruments and conduct their own experiments. The BR personnel train researchers wishing to use the facility and they are available to consult on experimental design/analysis or as collaborators in more detailed studies. Also, some equipment is too delicate to permit casual use, e.g. the high-sensitivity calorimeters, and users often collaborate with BR staff in the use of such instrumentation.
Design and Generation of Self-Assembling Peptide Virus-like Particles with Intrinsic GPCR Inhibitory Activity.In: Methods Mol Biol .. 2208: 135-148, 2021. [ Book Chapter ]
Structural plasticity of a transmembrane peptide allows self-assembly into biologically active nanoparticles.Proc. Natl. Acad. Sci. U.S.A. 108: 9798-803, 2011. [ Journal Article ]
Bisimidazoacridones: 2. Steady-state and time-resolved fluorescence studies of their diverse interactions with DNA.Photochem Photobiol. 78: 313-22, 2003. [ Journal Article ]
- Science. 351(6275): 831; pii: aad9421. doi: 10.1126/science.aad9421., 2016. [ Journal Article ]
- Nature. 541: 242-246, 2017. [ Journal Article ]
Sergey G Tarasov received M.S. degree in chemistry from Lomonosov Moscow State University (Russia) in 1975. He got his Ph.D.degree in chemistry of macromolecules, working on thermodynamics of multicomponent macromolecular systems in the laboratory of Prof. Yu.K.Godovski at the Karpov Institute of Physical Chemistry (Moscow, Russia). In 1997 he joined the Molecular Aspects of Drug Design section of ABL Research Program (head - Dr. C.J. Michejda) to study physico-chemical aspects of drug-DNA interactions. Since 2000 he is a Head of Biophysics Resource in the Structural Biophysics Laboratory (acting chief - Dr. K.J. Walters)
|Marzena A. Dyba||Research Associate III (Leidos)|