R. Andrew Byrd, Ph.D.
Dr. Byrd has been prominent in developing NMR-based structural biology for the study of proteins, nucleic acids, polysaccharides, and protein complexes. Current research focuses on deciphering allosteric mechanisms in the ubiquitination pathway regulating protein degradation/homeostatis and the roles of ADP ribosylation factors and GTPase activating proteins in regulating membrane trafficking.
As Chief of the Structural Biophysics Laboratory and Head of the Macromolecular NMR Section, Dr. Byrd contributes to the development and impact of structural biology within the CCR and oversees an active, multidisciplinary research effort utilizing biophysics and NMR spectroscopy to provide mechanistic understanding of complex biological processes
1) NMR, 2) structural biology, 3) biophysics, 4) ubiquitination
The Structural Biophysics Laboratory (SBL) focuses on solution structural biology and biophysics aimed at understanding and regulating the mechanism of action of proteins and nucleic acids. The major structural tool for the SBL is nuclear magnetic resonance (NMR) spectroscopy, and the Laboratory has a very strong interdisciplinary approach to understanding biochemical and biological mechanisms. Technologies incorporated in the research program include solution X-ray scattering, fluorescence and circular dichroism spectroscopy, mass spectrometry, calorimetry and novel binding methodologies including SPR and microscale thermophoresis. Research in the Byrd laboratory (Macromolecular NMR Section) focuses on protein-protein interactions in the ubiquitin-proteasome regulation pathway and the area of ADP-ribosylation factor (Arf) family of GTP-binding proteins and their regulators, the Arf GTPase-activating proteins (Arf GAPs). We seek to provide detailed structural and mechanistic insight, which we combine with collaborations in molecular and cell biology to inform and modulate biological function
Selected Key Publications
Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78.Mol. Cell. 34: 674-85, 2009. [ Journal Article ]
- EMBO J. 32: 2504-16, 2013. [ Journal Article ]
Targeted disruption of heparan sulfate interaction with hepatocyte and vascular endothelial growth factors blocks normal and oncogenic signaling.Cancer Cell. 22: 250-62, 2012. [ Journal Article ]
Improving the accuracy of NMR structures of large proteins using pseudocontact shifts as long-range restraints.J. Biomol. NMR. 28: 205-12, 2004. [ Journal Article ]
An NMR experiment for measuring methyl-methyl NOEs in 13C-labeled proteins with high resolution.J. Am. Chem. Soc. 120: 7617-25, 1998. [ Journal Article ]
Dr. Byrd received his Ph.D. from the University of South Carolina in 1977, specializing in high-resolution biomolecular NMR. He was a postdoctoral fellow and subsequently a research officer in the Molecular Biophysics Laboratory of the National Research Council of Canada, where he investigated biological membranes by solid-state NMR. Following a period as a senior investigator at the Center for Drugs and Biologics/FDA, he established the Macromolecular NMR Section of the ABL-Basic Research Program at the NCI-Frederick in 1992. He chaired the Experimental NMR Conference in 1992 and co-chaired the International Conference on Magnetic Resonance in Biological Systems in 1996. Dr. Byrd was appointed Chief of the Structural Biophysics Laboratory in the Center for Cancer Research, NCI at its establishment in 1999. He has served on multiple drug discovery panels for NCI and served as the Director of the Molecular Discovery Program from 2009-2011. In 2017, Dr. Byrd stepped down as Chief of SBL and continues as a Senior Investigator in the SBL.
|Jess Li Ph.D.||Biologist|
|Fa-An Chao Ph.D.||Postdoctoral Fellow (CRTA)|
|Ian J. Fucci Ph.D.||Postdoctoral Fellow (CRTA)|
|Domarin Khago Ph.D.||Postdoctoral Fellow (CRTA)|
|Kristen Snitchler B.Sc.||Postbaccalaureate Fellow (CRTA)|
|Olivier Soubias Ph.D.||Staff Scientist|
|Yue Zhang Ph.D.||Postdoctoral Fellow (Visiting)|
Scientist - Wanxin Pharmaceutical Techology, Suzhou, China
Medical School - University of Ohio
After spearheading the NUS project and development of NESTA-NMR, she is shifting into a new career path in data science
Dental School – Columbia University
Max Planck Institute, Goettingen, Germany
Proctor and Gamble Company, Cincinnati, Ohio
Novo Nordisk, Beijing, China
Research Associate-Protein Expression Purification and Crystallography Core, Department of Physiology and Biophysics, Case Western Reserve
Shan transitioned from research science into patent law. She is currently attending law school at George Washington University and works in patent law with Wilson Sonsini Goodrich & Rosati in Washington, D.C.
Assistant Professor, National Center for Biological Sciences, Bangalore, India
Transitioned to self-employment
Assistant Research Scientist, Institute for Bioscience and Biotechnology Research
Assistant Professor, CSIR-Central Drug Institute, Lucknow, India
Graduate student at Vanderbilt University (2009-2016)
Postdoctoral Fellow Yale University (2016-)
Research Assistant Professor, Department of Chemistry, University of Alabama-Huntsville
Associate Professor, Department of Chemistry, University of Alabama-Huntsville
Bruker Biospin, Applications Scientist
Biomolecular NMR Facility Manager, University of Colorado (2004-2007)
Associate Director NMR Facility/ Assistant Research Scientist, University of Iowa – Carver College of Medicine (2007-present)
Associate Professor, Department of Biochemistry and Molecular Genetics
Associate Professor, School of Medicine, Department of Biochemistry and Molecular Genetics, University of Colorado Denver
Ph.D. at Brown University (2010)
U.S. Agency for International Development (2011-present)
Professor Emeritus, Bar-Ilan University, Ramat-Gan, Israel
Professor, Molecular Biophysics Unit, Division of Biological Sciences, Indian Institute of Science, Bangalore, India
Director NMR Facility, Department of Chemistry and Biochemistry, University of California Santa Barbara
Associate Professor, Department of Biochemistry, School of Medicine, Wake Forest University (2000-2013)
Scientific Grant Writer, Nutrition Research Institute, University of North Carolina – Chapel Hill (2013-present)
Research Professor, University of Maryland School of Medicine (1996-2002)
Bruker Biospin, Senior Applications Scientist (2003-present)
Group represented with two posters (Domarin Khago and Andy Byrd) at the joint Euromar/ISMAR conference in Berlin, Germany, August 2019
Adiabatic relaxation dispersion methodology and applications described in recent papers led by Fa-An Chao: J. Magn. Res. 277:8-14 (2017); Emerging Topics in Life Sciences. 2:93-105 (2018); JACS 141(30):11881-11891 (2019)
Frank Chao gives promoted lecture on geometric approximation and HARD (Heteronuclear Adiabatic Relaxation Disperson) at Computational Aspects of Biomolecular NMR – Gordon Research Conference, Les Diablerets, Switzerland, June 2019.
Group represented with two posters (Chao: geoHARD and Zhang: ASAP1 PH domain and Nanodiscs) at 60th Experimental NMR Conference, Asilomar, CA, April, 2019
Dr. Byrd awarded August-Wilhelm Schneer Gastprofessor (second term) by the Technical Universität München, spends August-November 2018 at TUM, host Prof. Franz Hagn. Invited lectures at the Murnau Conference on Structural Biology, Murnau, Germany, Department of Biochemistry, Cambridge Univ., Cambridge, UK, and Dept. of Molecular Biology and Biochemistry, Medical Univ. of Graz, Austria.
Group represented with poster (Chakrabarti et al.) at XXVIII ICMRBS, Dublin, Ireland, August 2018.
Dr. Byrd gives invited lecture on “NMR Dynamics and Allostery in the Ubquitin Pathway” at the IBBR-NMR Day for local Washington area NMR groups, May 2018
Group represented with poster (Jess Li and Domarin Khago) at the Keystone Conference on Ubiquitin Signaling, January 2018.
Olivier Soubias joins the group as a Staff Scientist. Olivier brings expertise in membranes, lipids, and solid-state NMR that will contribute to all the projects, especially the nano disc work.
Group represented at XXVIIth ICMRBS, Kyoto, Japan: two posters and lecture by R.A. Byrd.
Fa-An Chao’s geoHARD paper published in J. Am.Chem. Soc. 2016: “Geometric approximation: a new computational approach to characterize protein dynamics from NMR adiabatic relaxation dispersion experiments”. JACS 138(23):7337-7345 (2016).
Group represented at 57th ENC, Pittsburgh: three posters and lecture by R.A. Byrd.
Dr. Byrd interviewed regarding research and NMR technology: Inhibiting Cancer Cells Using NMR (Bruker Corp.)
Collaborative work on ASAP1 PH domain with groups of Di Xia and Paul Randazzo group published: Structure 23(11):1977-88 (2015).
Dr. Byrd awarded August-Wilhelm Schneer Gastprofessor by Technical Universität München, spends January – March 2016 at TUM, host Profs. Michael Sattler and Franz Hagn.
New approach for analyzing dynamics in Intrinsically Disordered Proteins (IDPs) published by Michelle Gill in collaboration with Art Palmer, Columbia University: Phys. Chem. Chem. Phys. 18(8):5839-49 (2016).
Dr. Byrd serving as Chair, wwPDB Advisory Committee (2015-2017).
Group publishes new Non-Uniform Sampling software NESTA-NMR for reconstruction of NUS NMR data, work of Shangjin Sun and Michelle Gill: J. Biomol. NMR 62(1):105-17 (2015).