Tatiana S. Karpova, Ph.D.
Facility Head
Head, NCI Core Fluorescence Imaging Facility

Dr. Karpova manages the NCI Core Fluorescence Imaging Facility. This facility specializes in live imaging, image deconvolution and microscopy quantification methods such as FRET and FRAP. Dr. Karpova conducts research focusing on the regulation of gene expression by chromatin remodelers by observing protein interaction in vivo in live yeast cells. Her research interests include: -the intracellular dynamics of chromatin remodelers, transcriptional activators and co-activators; and -the role of nuclear localization on gene expression as observed in live yeast cells by microscopy.

Areas of Expertise
Fluorescence Imaging

Contact Info

Tatiana S. Karpova, Ph.D.
Center for Cancer Research
National Cancer Institute
Building 41, Room C615
Bethesda, MD 20892
301-435-4611
karpovat@mail.nih.gov

Dr. Karpova manages the NCI Core Fluorescence Imaging Facility. This facility specializes in live imaging, image deconvolution and microscopy quantification methods such as FRET and FRAP. Dr. Karpova conducts research focusing on the regulation of gene expression by chromatin remodelers by observing protein interaction in vivo in live yeast cells. Her research interests include: -the intracellular dynamics of chromatin remodelers, transcriptional activators and co-activators; and -the role of nuclear localization on gene expression as observed in live yeast cells by microscopy.

Selected Publications
  1. Poorey K, Viswanathan R, Carver MN, Karpova TS, Cirimotich SM, McNally JG, Bekiranov S, Auble DT.
    Science. 342: 369-72, 2013. [ Journal Article ]
  2. Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, Heliot L, McNally JG.
    Science. 319: 466-9, 2008. [ Journal Article ]
  3. Karpova TS, Chen TY, Sprague BL, McNally JG.
    EMBO Rep.. 5: 1064-70, 2004. [ Journal Article ]
  4. Karpova TS, Baumann CT, He L, Wu X, Grammer A, Lipsky P, Hager GL, McNally JG.
    J Microsc. 209: 56-70, 2003. [ Journal Article ]
  5. Waddle JA, Karpova TS, Waterston RH, Cooper JA.
    J. Cell Biol.. 132: 861-70, 1996. [ Journal Article ]

Tatiana Karpova obtained her Ph.D. in genetics from St. Petersburg State University (Leningrad, Russia) in 1984. From 1984-1991 she worked as an instructor and then as a senior lecturer in the Department of Genetics at St. Petersburg State University and conducted research in the field of genetic control of translation and translation termination factors in yeast. In 1991 Dr. Karpova was awarded the FEBS fellowship for research at Vrije University (Amsterdam, Holland). From 1992-1999 Dr. Karpova was a postdoctoral fellow in the Department of Cell Biology and Physiology, Washington University School of Medicine (St. Louis, MO). Her research involved genetics and microscopy of actin cytoskeleton in yeast. Dr. Karpova was one of the first scientists able to observe the actin cytoskeleton using green fluorescent protein (GFP) fusion protein markers in live yeast. In 1993-1995 she was awarded an American Heart Association Fellowship. From 1999 to date, Dr. Karpova has managed the NCI Core Fluorescence Imaging Facility.

Name Position
David Ball Ph.D. Research Fellow
Gunjan Mehta Ph.D. Postdoctoral Fellow (CRTA)

Research

Dr. Karpova manages the NCI Core Fluorescence Imaging Facility. This facility specializes in live imaging, image deconvolution and microscopy quantification methods such as FRET and FRAP. Dr. Karpova conducts research focusing on the regulation of gene expression by chromatin remodelers by observing protein interaction in vivo in live yeast cells. Her research interests include: -the intracellular dynamics of chromatin remodelers, transcriptional activators and co-activators; and -the role of nuclear localization on gene expression as observed in live yeast cells by microscopy.

Publications

Selected Publications
  1. Poorey K, Viswanathan R, Carver MN, Karpova TS, Cirimotich SM, McNally JG, Bekiranov S, Auble DT.
    Science. 342: 369-72, 2013. [ Journal Article ]
  2. Karpova TS, Kim MJ, Spriet C, Nalley K, Stasevich TJ, Kherrouche Z, Heliot L, McNally JG.
    Science. 319: 466-9, 2008. [ Journal Article ]
  3. Karpova TS, Chen TY, Sprague BL, McNally JG.
    EMBO Rep.. 5: 1064-70, 2004. [ Journal Article ]
  4. Karpova TS, Baumann CT, He L, Wu X, Grammer A, Lipsky P, Hager GL, McNally JG.
    J Microsc. 209: 56-70, 2003. [ Journal Article ]
  5. Waddle JA, Karpova TS, Waterston RH, Cooper JA.
    J. Cell Biol.. 132: 861-70, 1996. [ Journal Article ]

Biography

Tatiana Karpova obtained her Ph.D. in genetics from St. Petersburg State University (Leningrad, Russia) in 1984. From 1984-1991 she worked as an instructor and then as a senior lecturer in the Department of Genetics at St. Petersburg State University and conducted research in the field of genetic control of translation and translation termination factors in yeast. In 1991 Dr. Karpova was awarded the FEBS fellowship for research at Vrije University (Amsterdam, Holland). From 1992-1999 Dr. Karpova was a postdoctoral fellow in the Department of Cell Biology and Physiology, Washington University School of Medicine (St. Louis, MO). Her research involved genetics and microscopy of actin cytoskeleton in yeast. Dr. Karpova was one of the first scientists able to observe the actin cytoskeleton using green fluorescent protein (GFP) fusion protein markers in live yeast. In 1993-1995 she was awarded an American Heart Association Fellowship. From 1999 to date, Dr. Karpova has managed the NCI Core Fluorescence Imaging Facility.

Team

Name Position
David Ball Ph.D. Research Fellow
Gunjan Mehta Ph.D. Postdoctoral Fellow (CRTA)