Profile photo of Diana A. Stavreva, Ph.D.
Diana A. Stavreva, Ph.D.
Staff Scientist

Center for Cancer Research
National Cancer Institute

Building 41, Room B513
Bethesda, MD 20892
301-402-4801

I began my scientific career in the field of environmental mutagenesis. My work on DNA damage and repair at the single cell level motivated me to further explore single cell biology. I have been working on the development and implementation of a high-throughput, automated, cell-based screening protocol for the detection of biologically active endocrine-disrupting chemicals (EDCs) in environmental samples. Considering that exposure to EDCs has many deleterious effects, including abnormal breast development as well as an increased incidence of prostate and thyroid cancers, the efficient screening and elimination of EDCs from the environment could substantially reduce the risk of these ailments.

Areas of Expertise
1) transcription regulation, 2) chromatin dynamics, 3) nuclear receptor biology, 4) endocrine disrupting chemicals, 5) imaging, 6) genomics

Dynamic Transcription Regulation, Chromatin Dynamics, and Endocrine-Disrupting Chemicals (EDCs)

I began my scientific career in the field of environmental mutagenesis. My work on DNA damage and repair at the single cell level motivated me to further explore single cell biology. As a postdoctoral fellow in Dr. James McNally's lab at NCI I investigated the molecular mechanisms and functions of the glucocorticoid receptor (GR) rapid exchange with regulatory elements in living cells. After joining Dr. Gordon Hager's laboratory I became interested in dynamic transcription regulation in response to the naturally occurring transient activating signals. Using single cell analyses in a powerful combination with genome-wide approaches I have been investigating the effects of the ultradian (pulsatile) glucocorticoid release patterns on GR/chromatin interactions, chromatin accessibility, long-range interactions, and transcription regulation. These studies have significant implications for corticosteroid function in vivo and for steroid therapies in the clinic. More recently, reconnecting with my interest in the environmental sciences, I have been working on the development and implementation of a high-throughput, automated, cell-based screening protocol for the detection of biologically active endocrine-disrupting chemicals (EDCs) in environmental samples. Considering that exposure to EDCs has many deleterious effects, including abnormal breast development as well as an increased incidence of prostate and thyroid cancers, the efficient screening and elimination of EDCs from the environment could substantially reduce the risk of these ailments.

Scientific Focus Areas:
Cell Biology, Chromosome Biology, Genetics and Genomics, Molecular Biology and Biochemistry
Selected Publications
  1. Stavreva DA, George AA, Klausmeyer P, Varticovski L, Sack D, Voss TC, Schiltz RL, Blazer VS, Iwanowicz LR, Hager GL.
    Sci Rep. 2: 937, 2012. [ Journal Article ]
  2. Qiu,Y, Stavreva, DA, Luo,Y, Indrawan, A, Chang, M, and Hager, GL.
    J. Biol. Chem. 286: 7641-7, 2011. [ Journal Article ]
  3. Stavreva DA, Wiench M, John S, Conway-Campbell BL, McKenna MA, Pooley JR, Johnson TA, Voss TC, Lightman SL, Hager GL.
    Nat. Cell Biol.. 11: 1093-102, 2009. [ Journal Article ]
  4. Stavreva, DA, Kawasaki, M, Dundr, M, Koberna, K, Mueller, WG, Tsujimura-Takahashi, T, Komatsu, W, Hayano, T, Isobe, T, Raska, I, Misteli, T, Takahashi, N, and McNally JG.
    Mol. Cell. Biol. 26: 5131-45, 2006. [ Journal Article ]
  5. Stavreva, DA, Muller, WG, Hager, GL, Smith, CL, and McNally, JG.
    Mol. Cell. Biol. 24: 2682-97, 2004. [ Journal Article ]

Education:

Ph.D., Laboratory of Genetic Toxicology, Bulgarian Academy of Sciences, Sofia, Bulgaria, and Laboratory of Mutational Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 1998

M.S., Sofia University, Bulgaria, 1991

Research Experience:

Staff Scientist, Hormone Action and Oncogenesis Section, Laboratory of Receptor Biology and Gene Expression, CCR (2011 - present)

Research Fellow, Hormone Action and Oncogenesis Section, Laboratory of Receptor Biology and Gene Expression, CCR (2006 - 2011)

Visiting Fellow, Fluorescent Imaging Group, Laboratory of Receptor Biology and Gene Expression, CCR (2001 - 2006)

Researcher, Laboratory of Mutational Genetics, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic (1998 - 2001)

Summary

I began my scientific career in the field of environmental mutagenesis. My work on DNA damage and repair at the single cell level motivated me to further explore single cell biology. I have been working on the development and implementation of a high-throughput, automated, cell-based screening protocol for the detection of biologically active endocrine-disrupting chemicals (EDCs) in environmental samples. Considering that exposure to EDCs has many deleterious effects, including abnormal breast development as well as an increased incidence of prostate and thyroid cancers, the efficient screening and elimination of EDCs from the environment could substantially reduce the risk of these ailments.

Areas of Expertise
1) transcription regulation, 2) chromatin dynamics, 3) nuclear receptor biology, 4) endocrine disrupting chemicals, 5) imaging, 6) genomics

Research

Dynamic Transcription Regulation, Chromatin Dynamics, and Endocrine-Disrupting Chemicals (EDCs)

I began my scientific career in the field of environmental mutagenesis. My work on DNA damage and repair at the single cell level motivated me to further explore single cell biology. As a postdoctoral fellow in Dr. James McNally's lab at NCI I investigated the molecular mechanisms and functions of the glucocorticoid receptor (GR) rapid exchange with regulatory elements in living cells. After joining Dr. Gordon Hager's laboratory I became interested in dynamic transcription regulation in response to the naturally occurring transient activating signals. Using single cell analyses in a powerful combination with genome-wide approaches I have been investigating the effects of the ultradian (pulsatile) glucocorticoid release patterns on GR/chromatin interactions, chromatin accessibility, long-range interactions, and transcription regulation. These studies have significant implications for corticosteroid function in vivo and for steroid therapies in the clinic. More recently, reconnecting with my interest in the environmental sciences, I have been working on the development and implementation of a high-throughput, automated, cell-based screening protocol for the detection of biologically active endocrine-disrupting chemicals (EDCs) in environmental samples. Considering that exposure to EDCs has many deleterious effects, including abnormal breast development as well as an increased incidence of prostate and thyroid cancers, the efficient screening and elimination of EDCs from the environment could substantially reduce the risk of these ailments.

Scientific Focus Areas:
Cell Biology, Chromosome Biology, Genetics and Genomics, Molecular Biology and Biochemistry

Publications

Selected Publications
  1. Stavreva DA, George AA, Klausmeyer P, Varticovski L, Sack D, Voss TC, Schiltz RL, Blazer VS, Iwanowicz LR, Hager GL.
    Sci Rep. 2: 937, 2012. [ Journal Article ]
  2. Qiu,Y, Stavreva, DA, Luo,Y, Indrawan, A, Chang, M, and Hager, GL.
    J. Biol. Chem. 286: 7641-7, 2011. [ Journal Article ]
  3. Stavreva DA, Wiench M, John S, Conway-Campbell BL, McKenna MA, Pooley JR, Johnson TA, Voss TC, Lightman SL, Hager GL.
    Nat. Cell Biol.. 11: 1093-102, 2009. [ Journal Article ]
  4. Stavreva, DA, Kawasaki, M, Dundr, M, Koberna, K, Mueller, WG, Tsujimura-Takahashi, T, Komatsu, W, Hayano, T, Isobe, T, Raska, I, Misteli, T, Takahashi, N, and McNally JG.
    Mol. Cell. Biol. 26: 5131-45, 2006. [ Journal Article ]
  5. Stavreva, DA, Muller, WG, Hager, GL, Smith, CL, and McNally, JG.
    Mol. Cell. Biol. 24: 2682-97, 2004. [ Journal Article ]

Biography

Education:

Ph.D., Laboratory of Genetic Toxicology, Bulgarian Academy of Sciences, Sofia, Bulgaria, and Laboratory of Mutational Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 1998

M.S., Sofia University, Bulgaria, 1991

Research Experience:

Staff Scientist, Hormone Action and Oncogenesis Section, Laboratory of Receptor Biology and Gene Expression, CCR (2011 - present)

Research Fellow, Hormone Action and Oncogenesis Section, Laboratory of Receptor Biology and Gene Expression, CCR (2006 - 2011)

Visiting Fellow, Fluorescent Imaging Group, Laboratory of Receptor Biology and Gene Expression, CCR (2001 - 2006)

Researcher, Laboratory of Mutational Genetics, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic (1998 - 2001)