Yvona  Ward, Ph.D.
Yvona Ward, Ph.D.
Staff Scientist
Head, Microscopy Core Facility

Center for Cancer Research
National Cancer Institute

Building 37, Room 1066
Bethesda, MD 20892
301-594-2645

As head of the LGCP Microscopy Core Facility, Dr. Ward provides training, technical support and collaborative opportunities in epifluorescent microscopy, confocal microscopy, and live cell imaging. Dr. Ward also carries out independent research that focuses on the physiological role of CD97 in tumor progression and metastasis. Most recently, Dr. Ward has demonstrated that in its capacity as a receptor, CD97 heterodimerizes with LPA receptor to potentiate signaling via the Gα12/13 family of heterotrimeric G-proteins leading to Rho activation, ERK phosphorylation, increased lymphovascular invasion and metastasis.

Areas of Expertise
confocal microscopy, thyroid cancer progression and metastasis, prostate organoid culture, adhesion G-protein coupled receptor (GPCR) signaling, cancer mouse models

The Laboratory of Genitourinary Cancer Pathogenesis Microscopy Core Facility provides training, guidance, and state-of-the-art service to scientists in the LGCP and other laboratories at the NCI who require in vivo imaging or confocal microscopy techniques as part of their research efforts.

The core facility currently houses a Zeiss LSM 510 META confocal microscope capable of generating images with up to four different colors, an Olympus VivaView Fluorescence Incubator Microscope for long term live cell imaging, and a Zeiss AxioObserver.Z1 epifluorescent scope equipped with a digital camera and AxioVision software. Please refer to the Microscopy Core Facility website for a detailed description of equipment capability, sample images, publications, and scheduling time on the equipment. Image acquisition and analysis is provided free of charge.

Scientists who are fully trained in confocal microscopy work independently while inexperienced users are encouraged to arrange for training or to establish a collaboration with Yvona Ward.

In addition to managing the LGCP core facility, Dr. Ward conducts research in collaboration with Dr. Kathy Kelly on the role of CD97, an adhesion class GPCR, in tumor progression and metastasis.

Selected Publications
  1. Ward Y, Gupta S, Jensen P, Wartmann M, Davis RJ, Kelly K.
    Nature. 367: 651-4, 1994. [ Journal Article ]
  2. Ward Y, Wang W, Woodhouse E, Linnoila I, Liotta L, Kelly K.
    Mol. Cell. Biol.. 21: 5958-69, 2001. [ Journal Article ]
  3. Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler-Stevenson WG, Kelly K.
    Blood. 105: 2836-44, 2005. [ Journal Article ]
  4. Ward Y, Lake R, Yin JJ, Heger CD, Raffeld M, Goldsmith PK, Merino M, Kelly K.
    Cancer Res.. 71: 7301-11, 2011. [ Journal Article ]
  5. Ward Y, Lake R, Martin PL, Killian K, Salerno P, Wang T, Meltzer P, Merino M, Cheng S, Santoro M, Garcia-Rostan G, Kelly K.
    Oncogene. 32: 2726-38, 2013. [ Journal Article ]

Dr. Yvona Ward received her Ph.D. in 1992 from the University of Waterloo, Ontario, Canada. She then joined Dr. Kathy Kelly in the Laboratory of Pathology at the NCI where she conducted studies on PAC-1, a MAP Kinase phosphatase and investigated the role of the Ral pathway in tumorigenesis and metastasis. In 1999, Dr. Ward became the head of the Microinjection and Confocal Microscopy Core Facility in the Cell and Cancer Biology Branch of the NCI. Dr. Ward is currently head of the Laboratory of Genitourinary Cancer Pathogenesis Microscopy Core Facility.

Name Position
Ross Lake B.A. Research Biologist

Summary

As head of the LGCP Microscopy Core Facility, Dr. Ward provides training, technical support and collaborative opportunities in epifluorescent microscopy, confocal microscopy, and live cell imaging. Dr. Ward also carries out independent research that focuses on the physiological role of CD97 in tumor progression and metastasis. Most recently, Dr. Ward has demonstrated that in its capacity as a receptor, CD97 heterodimerizes with LPA receptor to potentiate signaling via the Gα12/13 family of heterotrimeric G-proteins leading to Rho activation, ERK phosphorylation, increased lymphovascular invasion and metastasis.

Areas of Expertise
confocal microscopy, thyroid cancer progression and metastasis, prostate organoid culture, adhesion G-protein coupled receptor (GPCR) signaling, cancer mouse models

Research

The Laboratory of Genitourinary Cancer Pathogenesis Microscopy Core Facility provides training, guidance, and state-of-the-art service to scientists in the LGCP and other laboratories at the NCI who require in vivo imaging or confocal microscopy techniques as part of their research efforts.

The core facility currently houses a Zeiss LSM 510 META confocal microscope capable of generating images with up to four different colors, an Olympus VivaView Fluorescence Incubator Microscope for long term live cell imaging, and a Zeiss AxioObserver.Z1 epifluorescent scope equipped with a digital camera and AxioVision software. Please refer to the Microscopy Core Facility website for a detailed description of equipment capability, sample images, publications, and scheduling time on the equipment. Image acquisition and analysis is provided free of charge.

Scientists who are fully trained in confocal microscopy work independently while inexperienced users are encouraged to arrange for training or to establish a collaboration with Yvona Ward.

In addition to managing the LGCP core facility, Dr. Ward conducts research in collaboration with Dr. Kathy Kelly on the role of CD97, an adhesion class GPCR, in tumor progression and metastasis.

Publications

Selected Publications
  1. Ward Y, Gupta S, Jensen P, Wartmann M, Davis RJ, Kelly K.
    Nature. 367: 651-4, 1994. [ Journal Article ]
  2. Ward Y, Wang W, Woodhouse E, Linnoila I, Liotta L, Kelly K.
    Mol. Cell. Biol.. 21: 5958-69, 2001. [ Journal Article ]
  3. Wang T, Ward Y, Tian L, Lake R, Guedez L, Stetler-Stevenson WG, Kelly K.
    Blood. 105: 2836-44, 2005. [ Journal Article ]
  4. Ward Y, Lake R, Yin JJ, Heger CD, Raffeld M, Goldsmith PK, Merino M, Kelly K.
    Cancer Res.. 71: 7301-11, 2011. [ Journal Article ]
  5. Ward Y, Lake R, Martin PL, Killian K, Salerno P, Wang T, Meltzer P, Merino M, Cheng S, Santoro M, Garcia-Rostan G, Kelly K.
    Oncogene. 32: 2726-38, 2013. [ Journal Article ]

Biography

Dr. Yvona Ward received her Ph.D. in 1992 from the University of Waterloo, Ontario, Canada. She then joined Dr. Kathy Kelly in the Laboratory of Pathology at the NCI where she conducted studies on PAC-1, a MAP Kinase phosphatase and investigated the role of the Ral pathway in tumorigenesis and metastasis. In 1999, Dr. Ward became the head of the Microinjection and Confocal Microscopy Core Facility in the Cell and Cancer Biology Branch of the NCI. Dr. Ward is currently head of the Laboratory of Genitourinary Cancer Pathogenesis Microscopy Core Facility.

Team

Name Position
Ross Lake B.A. Research Biologist