Valarie A. Barr, Ph.D.
Facility Head
Head, Microscopy Core Facility

Center for Cancer Research
National Cancer Institute

Building 37, Room 2035
Bethesda, MD 20892
301-496-9052

Coming soon.

Areas of Expertise
Coming soon.

The Microscopy Core facility provides state-of-the-art equipment, advice and training to researchers with particular expertise in live cell imaging. Most of the research done in the Core facility focuses on the movement of proteins involved in signal transduction pathways. In collaboration with Dr. Samelson, we study the movements of proteins initially found in signaling complexes that form at activated T cell receptors. These studies use a model system where chimeric proteins tagged with variants of GFP are visualized in Jurkat T cells. These cells are activated either by contact with antibody-coated coverslips or by contact with superantigen pulsed Raji B cells. Using this system we have studied the formation of signaling complexes, the movement of proteins involved in reorganization of the actin cytoskeleton and the trafficking of SLP-76, an important adaptor protein involved in T cell activation.

Selected Publications
  1. Balagopalan L, Barr VA, Kortum RL, Park AK, Samelson LE.
    J. Immunol.. 190: 3849-53, 2013. [ Journal Article ]
  2. Coussens NP, Hayashi R, Brown PH, Balagopalan L, Balbo A, Akpan I, Houtman JC, Barr VA, Schuck P, Appella E, Samelson LE.
    Mol. Cell. Biol.. 33: 4140-51, 2013. [ Journal Article ]
  3. Sherman E, Barr VA, Samelson LE.
    Methods. 59: 261-9, 2013. [ Journal Article ]
  4. Sherman E, Barr V, Samelson LE.
    Immunol. Rev.. 251: 21-35, 2013. [ Journal Article ]
  5. Zvezdova E, Lee J, El-Khoury D, Barr V, Akpan I, Samelson L, Love PE.
    Immunol. Cell Biol.. 2014. [ Journal Article ]

Dr. Barr received her Ph.D. degree from the Department of Cell Biology at the Johns Hopkins University School of Medicine in 1994. She then carried out postdoctoral research in the laboratory of Dr. Simeon Taylor in the Diabetes Branch, National Institute of Diabetes, Digestive and Kidney Diseases. She joined the Laboratory of Cellular and Molecular Biology as a Staff Scientist in 2001.

Name Position
Itoro O. Akpan M.S. Research Biologist

Summary

Coming soon.

Areas of Expertise
Coming soon.

Research

The Microscopy Core facility provides state-of-the-art equipment, advice and training to researchers with particular expertise in live cell imaging. Most of the research done in the Core facility focuses on the movement of proteins involved in signal transduction pathways. In collaboration with Dr. Samelson, we study the movements of proteins initially found in signaling complexes that form at activated T cell receptors. These studies use a model system where chimeric proteins tagged with variants of GFP are visualized in Jurkat T cells. These cells are activated either by contact with antibody-coated coverslips or by contact with superantigen pulsed Raji B cells. Using this system we have studied the formation of signaling complexes, the movement of proteins involved in reorganization of the actin cytoskeleton and the trafficking of SLP-76, an important adaptor protein involved in T cell activation.

Publications

Selected Publications
  1. Balagopalan L, Barr VA, Kortum RL, Park AK, Samelson LE.
    J. Immunol.. 190: 3849-53, 2013. [ Journal Article ]
  2. Coussens NP, Hayashi R, Brown PH, Balagopalan L, Balbo A, Akpan I, Houtman JC, Barr VA, Schuck P, Appella E, Samelson LE.
    Mol. Cell. Biol.. 33: 4140-51, 2013. [ Journal Article ]
  3. Sherman E, Barr VA, Samelson LE.
    Methods. 59: 261-9, 2013. [ Journal Article ]
  4. Sherman E, Barr V, Samelson LE.
    Immunol. Rev.. 251: 21-35, 2013. [ Journal Article ]
  5. Zvezdova E, Lee J, El-Khoury D, Barr V, Akpan I, Samelson L, Love PE.
    Immunol. Cell Biol.. 2014. [ Journal Article ]

Biography

Dr. Barr received her Ph.D. degree from the Department of Cell Biology at the Johns Hopkins University School of Medicine in 1994. She then carried out postdoctoral research in the laboratory of Dr. Simeon Taylor in the Diabetes Branch, National Institute of Diabetes, Digestive and Kidney Diseases. She joined the Laboratory of Cellular and Molecular Biology as a Staff Scientist in 2001.

Team

Name Position
Itoro O. Akpan M.S. Research Biologist