Joshua A. Welsh, Ph.D.

Joshua A. Welsh, Ph.D.

  • Center for Cancer Research
  • National Cancer Institute
Laboratory of Pathology


In my current position  I am helping to develop a scalable clinical pipeline that will enable the use of extracellular vesicles (EVs) as clinical biomarkers. This research includes exploring novel ways to label, detect, and purify EVs, and utilises a variety of techniques such as high-sensitivity nanoparticle sorting, single-fluorophore detection flow cytometry, multiplex analysis, sequencing, proteomics, and multi-dimensional analysis techniques.

Areas of Expertise

1) high-sensitivity flow cytometry 2) flow cytometer standardisation 3) multidimensional data analysis 4) translational medicine


Selected Recent Publications

Prospective use of high-refractive index materials for single molecule detection in flow cytometry

Welsh JA, Kepley J, Rosner A, Horak P, Berzofsky JA and Jones JC.
Sensors. 18(8): E2461, 2018. [ Journal Article ]

Systematic methodological evaluation of a multiplex bead-based flow cytometry assay for detection of extracellular vesicle surface signatures

Wiklander OPB, Bostancioglu RB, Welsh JA, Zickler AM, Murke F, Corso G, Felldin U, Hagey DW, Evertsson B, Liang X-M, Gustafsson MO, Mohammad DK, Wiek C, Hanenberg H, Bremer M, Gupta D, Björnstedt M, Giebel B, Nordin JZ, Jones JC, EL Andaloussi S and Görgens A.
Frontiers in Immunology. 9: Article 1326, 2018. [ Journal Article ]

Leukocyte extracellular vesicle concentration is inversely associated with liver fibrosis severity in NAFLD

Welsh JA, Scorletti E, Clough GF, Englyst NA and Byrne CD.
Journal of Leukocyte Biology. 104(3): 631-639, 2018. [ Journal Article ]

Extracellular Vesicle Flow Cytometry Analysis and Standardization

Welsh JA, Holloway JA, Wilkinson JS and Englyst NA.
Frontiers in Cell and Developmental Biology. 5: Article 78, 2017. [ Journal Article ]