Joshua A. Welsh, Ph.D.
- Center for Cancer Research
- National Cancer Institute
- Building 10, Room B1B51
- Bethesda, 20892
- 240-760-6673
- joshua.welsh@nih.gov
RESEARCH SUMMARY
In my current position I am helping to develop a scalable clinical pipeline that will enable the use of extracellular vesicles (EVs) as clinical biomarkers. This research includes exploring novel ways to label, detect, and purify EVs, and utilises a variety of techniques such as high-sensitivity nanoparticle sorting, single-fluorophore detection flow cytometry, multiplex analysis, sequencing, proteomics, and multi-dimensional analysis techniques.
Areas of Expertise
1) high-sensitivity flow cytometry 2) flow cytometer standardisation 3) multidimensional data analysis 4) translational medicine
Research
In my current position I am helping to develop a scalable clinical pipeline that will enable the use of extracellular vesicles (EVs) as clinical biomarkers. This research includes exploring novel ways to label, detect, and purify EVs, and utilises a variety of techniques such as high-sensitivity nanoparticle sorting, single-fluorophore detection flow cytometry, multiplex analysis, sequencing, proteomics, and multi-dimensional analysis techniques.
Publications
Prospective use of high-refractive index materials for single molecule detection in flow cytometry
Systematic methodological evaluation of a multiplex bead-based flow cytometry assay for detection of extracellular vesicle surface signatures
Leukocyte extracellular vesicle concentration is inversely associated with liver fibrosis severity in NAFLD
Extracellular Vesicle Flow Cytometry Analysis and Standardization
Biography
Joshua A. Welsh, Ph.D.
I obtained my Ph.D. from the University of Southampton, where I researched the development of high-sensitivity flow cytometers with industry. Along with equipment development I created novel particle scattering models to allow standardization of small particle detection using flow cytometry. I would go on to apply these techniques to a number of translational studies including: acute ischemic stroke, cardiac bypass surgery, nonalcoholic fatty liver disease and allergy development.
Awards:
- Federal Technology Transfer Award, NIH, 2018
- Summer Research Mentor Award, NIH, 2018
- 2nd Prize for Best Oral Presentations, UKEV Forum, 2017
- Student Travel Award, International Society for Advancement of Cytometry, 2016
- International Conference Attendance Fund, University of Southampton, 2016
- Researcher Links/Newton Award, British Council, 2016
- Student Travel Award, International Society for Advancement of Cytometry, 2015
- International Conference Attendance Fund, University of Southampton, 2015
- Researcher Links Award, British Council, 2015
- Young Extraordinary Brits Award: Innovation, Jack Wills, 2014
- Best Overall 3rd Year Experimental Project, University of Leicester, 2013
- Awarded 1st Year University Scholarship, University of Leicester, 2010
Resources
- Up to date versions of my software - https://nanopass.ccr.cancer.gov
- ISAC-ISEV-ISTH FCM Working Group