Sriram  Subramaniam, Ph.D.
Sriram Subramaniam, Ph.D.
Senior Investigator
Head, Biophysics Section

Center for Cancer Research
National Cancer Institute

Building 50, Room 4306
Bethesda, MD 20892-8008
301-594-2062

We develop and apply novel methods in 3D electron microscopy and image processing to determine structures of a variety of macromolecular assemblies of fundamental biomedical interest. Main areas of current interest include: structural analysis of membrane transporters and dynamic protein complexes involved in signaling and metabolism, structure and structural variations of enveloped virus glycoproteins and their applicability to vaccine design, 3D subcellular imaging with correlative light and electron microscopy to explore host-pathogen interactions, and the development of methods for high-resolution cryo-EM.

For more information, visit these websites:

Areas of Expertise
1) cryo-electron microscopy 2) protein structure, 3) HIV entry, 4) correlative light and electron microscopy, 5) electron tomography, 6) automated image processing

Three-Dimensional Electron Microscopy at Molecular Resolution

For a more detailed description of our research program, please visit our High Resolution Electron Microscopy website at http://electron.nci.nih.gov.

The long-term mission of our research program is to obtain an integrated, quantitative understanding of cells and viruses at molecular resolution. We take an interdisciplinary approach to this problem by combining novel technologies for three-dimensional (3D) imaging with computational and cell biological tools. Our research efforts are presently focused on three areas: (1) determination of the structure and mechanisms underlying neutralization and cellular entry of HIV, (2) the development of automated, high-throughput workflows for structure determination of small, dynamic molecular complexes, and (3) the development of novel technologies for 3D imaging of cells and tissues, with particular emphasis on methods for understanding and diagnosing structural signatures of signal transduction and disease progression.

Scientific Focus Areas:
Cell Biology, Chemical Biology, Computational Biology, Structural Biology, Virology
Selected Recent Publications
  1. Frank GA, Narayan K, Bess JW, Del Prete GQ, Wu X, Moran A, Hartnell LM, Earl LA, Lifson JD, and Subramaniam S.
    Nat Commun. 8: 5854, 2015. [ Journal Article ]
  2. Meyerson JR, Kumar J, Chittori S, Rao P, Pierson J, Bartesaghi A, Mayer ML, and Subramaniam S.
    Nature. 514: 328-34, 2014. [ Journal Article ]
  3. Bartesaghi A, Matthies D, Banerjee S, Merk A, and Subramaniam S.
    Proc. Natl. Acad. Sci. U.S.A. 111: 11709-14, 2014. [ Journal Article ]
  4. Do T, Murphy G, Earl LA, Del Prete GQ, Grandinetti G, Li G, Estes J, Rao P, Trubey C, Thomas J, Spector J, Bliss D, Nath A, Lifson JD, and Subramaniam S.
    J.Virol. 88: 10327-39, 2014. [ Journal Article ]
  5. Bartesaghi A, Merk A, Borgnia MJ, Milne JL, and Subramaniam S.
    Nat. Struct. Mol. Biol. 20: 1352-7, 2013. [ Journal Article ]

Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research.

Name Position
Soojay Banerjee Ph.D. Research Biochemist (Contr)
Alberto Bartesaghi, Ph.D. Staff Scientist
Daniel Cooper Web Manager (Contr)
Lesley Earl Ph.D. Postdoctoral Fellow (CRTA)
Veronica Falconieri Illustrator (Contr.)
Andrei Florescu Data Manager (Contr)
Gabriel Frank Ph.D. Postdoctoral Fellow (Visiting)
Lisa Hartnell Research Biologist
Bobby Hollingsworth Special Volunteer
Martin Kessel Ph.D. Special Volunteer
Doreen Matthies Ph.D. Postdoctoral Fellow (Visiting)
Joel Meyerson Ph.D. Postdoctoral Fellow (CRTA)
Kira Podolsky Postbaccalaureate Fellow
Prashant Rao Postbaccalaureate Fellow
Erin Tran Ph.D. Research Fellow
Madhvi Venkatesh Predoctoral Fellow

Summary

We develop and apply novel methods in 3D electron microscopy and image processing to determine structures of a variety of macromolecular assemblies of fundamental biomedical interest. Main areas of current interest include: structural analysis of membrane transporters and dynamic protein complexes involved in signaling and metabolism, structure and structural variations of enveloped virus glycoproteins and their applicability to vaccine design, 3D subcellular imaging with correlative light and electron microscopy to explore host-pathogen interactions, and the development of methods for high-resolution cryo-EM.

For more information, visit these websites:

Areas of Expertise
1) cryo-electron microscopy 2) protein structure, 3) HIV entry, 4) correlative light and electron microscopy, 5) electron tomography, 6) automated image processing

Research

Three-Dimensional Electron Microscopy at Molecular Resolution

For a more detailed description of our research program, please visit our High Resolution Electron Microscopy website at http://electron.nci.nih.gov.

The long-term mission of our research program is to obtain an integrated, quantitative understanding of cells and viruses at molecular resolution. We take an interdisciplinary approach to this problem by combining novel technologies for three-dimensional (3D) imaging with computational and cell biological tools. Our research efforts are presently focused on three areas: (1) determination of the structure and mechanisms underlying neutralization and cellular entry of HIV, (2) the development of automated, high-throughput workflows for structure determination of small, dynamic molecular complexes, and (3) the development of novel technologies for 3D imaging of cells and tissues, with particular emphasis on methods for understanding and diagnosing structural signatures of signal transduction and disease progression.

Scientific Focus Areas:
Cell Biology, Chemical Biology, Computational Biology, Structural Biology, Virology

Publications

Selected Recent Publications
  1. Frank GA, Narayan K, Bess JW, Del Prete GQ, Wu X, Moran A, Hartnell LM, Earl LA, Lifson JD, and Subramaniam S.
    Nat Commun. 8: 5854, 2015. [ Journal Article ]
  2. Meyerson JR, Kumar J, Chittori S, Rao P, Pierson J, Bartesaghi A, Mayer ML, and Subramaniam S.
    Nature. 514: 328-34, 2014. [ Journal Article ]
  3. Bartesaghi A, Matthies D, Banerjee S, Merk A, and Subramaniam S.
    Proc. Natl. Acad. Sci. U.S.A. 111: 11709-14, 2014. [ Journal Article ]
  4. Do T, Murphy G, Earl LA, Del Prete GQ, Grandinetti G, Li G, Estes J, Rao P, Trubey C, Thomas J, Spector J, Bliss D, Nath A, Lifson JD, and Subramaniam S.
    J.Virol. 88: 10327-39, 2014. [ Journal Article ]
  5. Bartesaghi A, Merk A, Borgnia MJ, Milne JL, and Subramaniam S.
    Nat. Struct. Mol. Biol. 20: 1352-7, 2013. [ Journal Article ]

Biography

Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research.

Team

Name Position
Soojay Banerjee Ph.D. Research Biochemist (Contr)
Alberto Bartesaghi, Ph.D. Staff Scientist
Daniel Cooper Web Manager (Contr)
Lesley Earl Ph.D. Postdoctoral Fellow (CRTA)
Veronica Falconieri Illustrator (Contr.)
Andrei Florescu Data Manager (Contr)
Gabriel Frank Ph.D. Postdoctoral Fellow (Visiting)
Lisa Hartnell Research Biologist
Bobby Hollingsworth Special Volunteer
Martin Kessel Ph.D. Special Volunteer
Doreen Matthies Ph.D. Postdoctoral Fellow (Visiting)
Joel Meyerson Ph.D. Postdoctoral Fellow (CRTA)
Kira Podolsky Postbaccalaureate Fellow
Prashant Rao Postbaccalaureate Fellow
Erin Tran Ph.D. Research Fellow
Madhvi Venkatesh Predoctoral Fellow