Sergei  Pletnev, Ph.D.
Sergei Pletnev, Ph.D.
Staff Scientist (Contr)

Center for Cancer Research
National Cancer Institute

Building 202, Room E-179
Argonne, IL 60439
630-252-8230

Dr. Pletnev's research focuses on structural studies of fluorescent proteins (FPs), a class of natural and bio-engineered proteins that are used as non-invasive fluorescent biomarkers and biosensors. The primary application of FPs is in vivo measurements of certain physical and chemical parameters of cells or particular cellular compartments (e.g., pH, concentration of specific ions), monitoring of gene expression, protein labeling, and tissue visualization. Dr. Pletnev's studies are aimed at improving the physical and chemical characteristics of FPs that are important for their successful practical application: brightness, photo- and pH stability, ion sensitivity, phototoxicity, photoswitchability, time-resolved properties, etc.

Areas of Expertise
1) structural and molecular biology, 2) macromolecular crystallography, 3) protein engineering, 4) fluorescent proteins, 5) non-invasive biomarkers and biosensors

Dr. Pletnev's current research focuses on structural studies of fluorescent proteins (FPs), a class of natural and bio-engineered proteins that are used as non-invasive fluorescent biomarkers and biosensors. The primary application of FPs is in vivo measurements of certain physical and chemical parameters of cells or particular cellular compartments (e.g., pH, concentration of specific ions), monitoring of gene expression, protein labeling, and tissue visualization. Dr. Pletnev's structural studies are aimed at improving the physical and chemical characteristics of FPs that are important for their successful practical application: brightness, photo- and pH stability, ion sensitivity, phototoxicity, photoswitchability, time-resolved properties, etc. Dr. Pletnev conducts his research in collaboration with two leading institutions in the field of fluorescent proteins: the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences and Albert-Einstein College of Medicine.

Selected Publications
  1. Pletnev S, Subach FV, Verkhusha VV, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 70: 31-9, 2014. [ Journal Article ]
  2. Pletnev VZ, Pletneva NV, Lukyanov KA, Souslova EA, Fradkov AF, Chudakov DM, Chepurnykh T, Yampolsky IV, Wlodawer A, Dauter Z, Pletnev S.
    Acta Crystallogr. D Biol. Crystallogr. 69: 1850-60, 2013. [ Journal Article ]
  3. Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Lukyanov S, Martynov VI, Arhipova S, Artemyev I, Wlodawer A, Dauter Z, Pletnev S.
    Acta Crystallogr. D Biol. Crystallogr. 69: 1005-12, 2013. [ Journal Article ]
  4. Pletnev S, Subach FV, Dauter Z, Wlodawer A, Verkhusha VV.
    J. Mol. Biol. 417: 144-51, 2012. [ Journal Article ]
  5. Pletnev S, Pletneva NV, Souslova EA, Chudakov DM, Lukyanov S, Wlodawer A, Dauter Z, Pletnev V.
    Acta Crystallogr. D Biol. Crystallogr. 68: 1088-97, 2012. [ Journal Article ]

Dr. Pletnev received his Ph.D. in 1997 from the Shubnikov Institute for Crystallography, Russian Academy of Sciences, under the supervision of Professor Emil Harutyunyan. He received his postdoctoral training in the laboratory of Professor Michael Rossmann at Purdue University working, among the others, on the projects that revealed the structures of Sindbis and Dengue viruses. In 2001, Dr. Pletnev joined the Macromolecular Crystallography Laboratory.

Summary

Dr. Pletnev's research focuses on structural studies of fluorescent proteins (FPs), a class of natural and bio-engineered proteins that are used as non-invasive fluorescent biomarkers and biosensors. The primary application of FPs is in vivo measurements of certain physical and chemical parameters of cells or particular cellular compartments (e.g., pH, concentration of specific ions), monitoring of gene expression, protein labeling, and tissue visualization. Dr. Pletnev's studies are aimed at improving the physical and chemical characteristics of FPs that are important for their successful practical application: brightness, photo- and pH stability, ion sensitivity, phototoxicity, photoswitchability, time-resolved properties, etc.

Areas of Expertise
1) structural and molecular biology, 2) macromolecular crystallography, 3) protein engineering, 4) fluorescent proteins, 5) non-invasive biomarkers and biosensors

Research

Dr. Pletnev's current research focuses on structural studies of fluorescent proteins (FPs), a class of natural and bio-engineered proteins that are used as non-invasive fluorescent biomarkers and biosensors. The primary application of FPs is in vivo measurements of certain physical and chemical parameters of cells or particular cellular compartments (e.g., pH, concentration of specific ions), monitoring of gene expression, protein labeling, and tissue visualization. Dr. Pletnev's structural studies are aimed at improving the physical and chemical characteristics of FPs that are important for their successful practical application: brightness, photo- and pH stability, ion sensitivity, phototoxicity, photoswitchability, time-resolved properties, etc. Dr. Pletnev conducts his research in collaboration with two leading institutions in the field of fluorescent proteins: the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences and Albert-Einstein College of Medicine.

Publications

Selected Publications
  1. Pletnev S, Subach FV, Verkhusha VV, Dauter Z.
    Acta Crystallogr. D Biol. Crystallogr. 70: 31-9, 2014. [ Journal Article ]
  2. Pletnev VZ, Pletneva NV, Lukyanov KA, Souslova EA, Fradkov AF, Chudakov DM, Chepurnykh T, Yampolsky IV, Wlodawer A, Dauter Z, Pletnev S.
    Acta Crystallogr. D Biol. Crystallogr. 69: 1850-60, 2013. [ Journal Article ]
  3. Pletneva NV, Pletnev VZ, Souslova E, Chudakov DM, Lukyanov S, Martynov VI, Arhipova S, Artemyev I, Wlodawer A, Dauter Z, Pletnev S.
    Acta Crystallogr. D Biol. Crystallogr. 69: 1005-12, 2013. [ Journal Article ]
  4. Pletnev S, Subach FV, Dauter Z, Wlodawer A, Verkhusha VV.
    J. Mol. Biol. 417: 144-51, 2012. [ Journal Article ]
  5. Pletnev S, Pletneva NV, Souslova EA, Chudakov DM, Lukyanov S, Wlodawer A, Dauter Z, Pletnev V.
    Acta Crystallogr. D Biol. Crystallogr. 68: 1088-97, 2012. [ Journal Article ]

Biography

Dr. Pletnev received his Ph.D. in 1997 from the Shubnikov Institute for Crystallography, Russian Academy of Sciences, under the supervision of Professor Emil Harutyunyan. He received his postdoctoral training in the laboratory of Professor Michael Rossmann at Purdue University working, among the others, on the projects that revealed the structures of Sindbis and Dengue viruses. In 2001, Dr. Pletnev joined the Macromolecular Crystallography Laboratory.