Towards Better Understanding of KSHV Life Cycle: from Transcription and Posttranscriptional Regulations to Pathogenesis 2) HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein

Virologica Sinica Cover April 2019

1) Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus-8 (HHV-8), is etiologically linked to the development of Kaposi’s sarcoma, primary effusion lymphoma, and multicentric Castleman’s disease. In this review, Yan and Majerciak et al. summarize the current research status on the biology of latent and lytic viral infection, the regulation of viral life cycles and the related pathogenesis.

2) Human papillomavirus 18 (HPV18) E6 and E7 oncogenes are transcribed as a single bicistronic E6E7 pre-mRNA. The E6 ORF region in the bicistronic E6E7 pre-mRNA contains an intron. Splicing of this intron disrupts the E6 ORF integrity and produces a spliced E6*I RNA for efficient E7 translation. Brant et al. identified two alternative branch sites in the HPV18 E6 intron which selection correlates to the efficiency of E6*I splicing and the production of E6 and E7 oncoproteins during productive or oncogenic HPV infection.

Retroviral Replication Laboratory

1) Yan and Majerciak et al., Virologica Sinica Volume 34 Issue 2, pages 135–161,

2) Ayslan Castro Brant et al., Virologica Sinica, Volume 34, Issue 2, pages 211–221,

Published Date: 
April, 2019