Structural models of Recombinant Immunotoxins.
A. SS1P consists of the disulfide-stabilized heavy chain Fv (VH) (magenta) and light chain Fv (VL) (Cyan) of the antibody SS1P. The VH is linked to a 38-kDa fragment of PE38 that is divided into domain II (gray), domain III (yellow), and part of domain Ib from native PE38. B. SS1-LO10R. 24-kDa fragment of PE24 with six point mutations in domain III designed to eliminate binding to B-cell receptor. Point mutations are marked with red balls. C. LMB-T20. PE24 with six point mutations in domain III designed to diminish T-cell epitopes. D. LMB-T14. PE24 with 10 point mutations in domain III designed to diminish B and T cell epitopes. All models are hypothetical arrangements based on the structures of native PE and immunoglobulin G; they do not represent actual structure determinations.
Ronit Mazor1, Masanori Onda1, Dong Park1,3, Selamawit Addissie1, Laiman Xiang1,*, Jingli Zhang2, Raffit Hassan2 and Ira Pastan1
1 Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
2 Thoracic and GI Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
3 New Business Development Department, Medytox Inc., Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea
Oncotarget, Vol. 7, No. 21