Maria Kireeva, Ph.D.
Staff Scientist

Center for Cancer Research
National Cancer Institute

Building 539, Room 245
Frederick, MD 21702-1201
301-846-1799

Dr. Kireeva’s research is focused on mechanisms promoting faithful transcription of genomic DNA in bacteria, yeast and mammals. She is investigating fidelity of mRNA synthesis using biochemically defined and cell extract-based systems. She participates in a collaborative effort to reveal mechanisms and biological effects of misincorporations, insertions and deletions during transcription elongation and explores effects of DNA damage on RNA polymerase II progression. Dr. Kireeva is actively involved in development of new therapeutic applications of native and chemically-modified RNA-based nanoparticles. In an effort to find new safe anti-HCV therapy, she established that the ability of nuclear RNA polymerase II to metabolize anti-viral NTP analogues may contribute to the compound toxicity.

Areas of Expertise
in vitro transcription, DNA repair, protein purification, pre-steady-state kinetic analyses
Selected Publications
  1. Kireeva ML, Kashlev M, Burton ZF.
    Chem. Rev.. 113: 8325-30, 2013. [ Journal Article ]
  2. Kireeva ML, Opron K, Seibold SA, Domecq C, Cukier RI, Coulombe B, Kashlev M, Burton ZF.
    BMC Biophys. 5: 11, 2012. [ Journal Article ]
  3. Afonin KA, Kireeva M, Grabow WW, Kashlev M, Jaeger L, Shapiro BA.
    Nano Lett.. 12: 5192-5, 2012. [ Journal Article ]
  4. Kireeva M, Kashlev M, Burton ZF.
    Biochim. Biophys. Acta. 1799: 389-401, 2009. [ Journal Article ]
  5. Kireeva ML, Kashlev M.
    Proc. Natl. Acad. Sci. U.S.A.. 106: 8900-5, 2009. [ Journal Article ]

Summary

Dr. Kireeva’s research is focused on mechanisms promoting faithful transcription of genomic DNA in bacteria, yeast and mammals. She is investigating fidelity of mRNA synthesis using biochemically defined and cell extract-based systems. She participates in a collaborative effort to reveal mechanisms and biological effects of misincorporations, insertions and deletions during transcription elongation and explores effects of DNA damage on RNA polymerase II progression. Dr. Kireeva is actively involved in development of new therapeutic applications of native and chemically-modified RNA-based nanoparticles. In an effort to find new safe anti-HCV therapy, she established that the ability of nuclear RNA polymerase II to metabolize anti-viral NTP analogues may contribute to the compound toxicity.

Areas of Expertise
in vitro transcription, DNA repair, protein purification, pre-steady-state kinetic analyses

Publications

Selected Publications
  1. Kireeva ML, Kashlev M, Burton ZF.
    Chem. Rev.. 113: 8325-30, 2013. [ Journal Article ]
  2. Kireeva ML, Opron K, Seibold SA, Domecq C, Cukier RI, Coulombe B, Kashlev M, Burton ZF.
    BMC Biophys. 5: 11, 2012. [ Journal Article ]
  3. Afonin KA, Kireeva M, Grabow WW, Kashlev M, Jaeger L, Shapiro BA.
    Nano Lett.. 12: 5192-5, 2012. [ Journal Article ]
  4. Kireeva M, Kashlev M, Burton ZF.
    Biochim. Biophys. Acta. 1799: 389-401, 2009. [ Journal Article ]
  5. Kireeva ML, Kashlev M.
    Proc. Natl. Acad. Sci. U.S.A.. 106: 8900-5, 2009. [ Journal Article ]