Lynn Thomason, Ph.D.
Staff Scientist (Contr)

Center for Cancer Research
National Cancer Institute

Building 539, Room 243
Frederick, MD 21702-1201
301-846-7206

Dr. Thomason has extensive knowledge of microbial genetics. She uses recombineering to generate genetically modified genomes for functional genomic studies, as well as investigating the molecular mechanism of recombineering. Dr Thomason is a group leader for the Basic Sciences Program (Leidos Biomedical, Inc.), mentors technicians, postdocs and summer students in MCG, attends and presents in MCG and GRCBL group meetings and other scientific meetings, assists other scientists, and publishes experimental results.

Areas of Expertise
microbial genetics, recombineering: in vivo genetic engineering, viral-host interactions, Escherichia coli, bacteriophage λ
Selected Publications
  1. Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
    Mol. Microbiol.. 88: 906-20, 2013. [ Journal Article ]
  2. Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
    Nucleic Acids Res.. 41: e204, 2013. [ Journal Article ]
  3. Thomason LC, Sawitzke JA, Li X, Costantino N, Court DL.
    Curr Protoc Mol Biol. 106: 1.16.1-1.16.39, 2014. [ Journal Article ]
  4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL.
    Nat Protoc. 4: 206-23, 2009. [ Journal Article ]
  5. Sawitzke JA, Costantino N, Li XT, Thomason LC, Bubunenko M, Court C, Court DL.
    J. Mol. Biol.. 407: 45-59, 2011. [ Journal Article ]

Lynn Thomason obtained an undergraduate degree in general studies in the physical sciences (1983) and a provisional teaching certificate (1984), both from Washington State University. She earned both a M.S. (1987) and a Ph.D. (1993) from the Department of Chemistry at the University of Oregon, where she was a graduate student in the laboratory of Dr. Franklin W. Stahl in the Institute of Molecular Biology. Her thesis work demonstrated that, under some conditions, bacteriophage lambda circular monomer chromosomes are packaged in vivo. After additional time in the Stahl laboratory, in 1998 Lynn became a postdoctoral fellow in the laboratory of Dr. Richard Calendar at University of California at Berkeley where, in collaboration with Dr. David Ow of the Agricultural Research Service of the U.S. Department of Agriculture, she demonstrated activity of the bacteriophage PhiC31 site-specific recombination system in the fission yeast Schizosaccharomyces pombe. In 2001 Dr. Thomason came to the National Cancer Institute at Frederick as a cancer research award (CRTA) trainee in Dr. Donald Court’s lab, where she learned recombineering and helped further develop the technology. After completion of the CRTA program Lynn remained in Dr. Court's lab and is presently employed as a scientist II by Leidos Biomedical, Inc.

Summary

Dr. Thomason has extensive knowledge of microbial genetics. She uses recombineering to generate genetically modified genomes for functional genomic studies, as well as investigating the molecular mechanism of recombineering. Dr Thomason is a group leader for the Basic Sciences Program (Leidos Biomedical, Inc.), mentors technicians, postdocs and summer students in MCG, attends and presents in MCG and GRCBL group meetings and other scientific meetings, assists other scientists, and publishes experimental results.

Areas of Expertise
microbial genetics, recombineering: in vivo genetic engineering, viral-host interactions, Escherichia coli, bacteriophage λ

Publications

Selected Publications
  1. Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
    Mol. Microbiol.. 88: 906-20, 2013. [ Journal Article ]
  2. Li XT, Thomason LC, Sawitzke JA, Costantino N, Court DL.
    Nucleic Acids Res.. 41: e204, 2013. [ Journal Article ]
  3. Thomason LC, Sawitzke JA, Li X, Costantino N, Court DL.
    Curr Protoc Mol Biol. 106: 1.16.1-1.16.39, 2014. [ Journal Article ]
  4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL.
    Nat Protoc. 4: 206-23, 2009. [ Journal Article ]
  5. Sawitzke JA, Costantino N, Li XT, Thomason LC, Bubunenko M, Court C, Court DL.
    J. Mol. Biol.. 407: 45-59, 2011. [ Journal Article ]

Biography

Lynn Thomason obtained an undergraduate degree in general studies in the physical sciences (1983) and a provisional teaching certificate (1984), both from Washington State University. She earned both a M.S. (1987) and a Ph.D. (1993) from the Department of Chemistry at the University of Oregon, where she was a graduate student in the laboratory of Dr. Franklin W. Stahl in the Institute of Molecular Biology. Her thesis work demonstrated that, under some conditions, bacteriophage lambda circular monomer chromosomes are packaged in vivo. After additional time in the Stahl laboratory, in 1998 Lynn became a postdoctoral fellow in the laboratory of Dr. Richard Calendar at University of California at Berkeley where, in collaboration with Dr. David Ow of the Agricultural Research Service of the U.S. Department of Agriculture, she demonstrated activity of the bacteriophage PhiC31 site-specific recombination system in the fission yeast Schizosaccharomyces pombe. In 2001 Dr. Thomason came to the National Cancer Institute at Frederick as a cancer research award (CRTA) trainee in Dr. Donald Court’s lab, where she learned recombineering and helped further develop the technology. After completion of the CRTA program Lynn remained in Dr. Court's lab and is presently employed as a scientist II by Leidos Biomedical, Inc.